MCF-7 is a

breast cancer Breast cancer is cancer that develops from breast tissue. Signs of breast cancer may include a lump in the breast, a change in breast shape, dimpling of the skin, milk rejection, fluid coming from the nipple, a newly inverted nipple, or ...

cell line isolated in 1970 from a 69-year-old

White White is the lightness, lightest color and is achromatic (having no hue). It is the color of objects such as snow, chalk, and milk, and is the opposite of black. White objects fully diffuse reflection, reflect and scattering, scatter all the ...

woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. The Michigan Cancer Foundation is now known as the Barbara Ann Karmanos Cancer Institute. Prior to MCF-7, it was not possible for cancer researchers to obtain a mammary cell line that was capable of living longer than a few months. The patient, Frances Mallon died in 1970. Her cells were the source of much of current knowledge about breast cancer. At the time of sampling, she was a nun in the convent of Immaculate Heart of Mary in

Monroe, Michigan Monroe is the largest city and county seat of Monroe County in the U.S. state of Michigan. Monroe had a population of 20,462 in the 2020 census. The city is bordered on the south by Monroe Charter Township, but the two are administered autonom ...

under the name of Sister Catherine Frances. MCF-7 and two other breast cancer cell lines, named T-47D and MDA-MB-231, account for more than two-thirds of all abstracts reporting studies on mentioned breast cancer cell lines, as concluded from a

Medline MEDLINE (Medical Literature Analysis and Retrieval System Online, or MEDLARS Online) is a bibliographic database of life sciences and biomedical information. It includes bibliographic information for articles from academic journals covering medic ...

-based survey.

Characteristics of MCF-7 cells

MCF-7 cells have the following characteristics: *Primary tumor (invasive

breast The breast is one of two prominences located on the upper ventral region of a primate's torso. Both females and males develop breasts from the same embryological tissues. In females, it serves as the mammary gland, which produces and s ...

ductal carcinoma) *Originate from pleural effusion * Estrogen receptors present *Proliferative response to estrogens *Presence of

progesterone receptor The progesterone receptor (PR), also known as NR3C3 or nuclear receptor subfamily 3, group C, member 3, is a protein found inside cells. It is activated by the steroid hormone progesterone. In humans, PR is encoded by a single ''PGR'' gene res ...

s *Cannot have '' ERBB2'' gene amplification (with Her2/neu protein overexpression) *Tumorigenic in mice but only with

estrogen Estrogen or oestrogen is a category of sex hormone responsible for the development and regulation of the female reproductive system and secondary sex characteristics. There are three major endogenous estrogens that have estrogenic hormonal a ...

supplementation if engrafted into the subcutaneous fat or mammary fat pad *Tumorigenic in mice without estrogen supplementation if engrafted intraductally *Luminal epithelial phenotype This cell line retained several characteristics of differentiated mammary epithelium, including the ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. Tumor necrosis factor alpha ( TNF alpha) inhibits the growth of MCF-7 breast cancer cells. Treatment with anti-estrogens can modulate the secretion of insulin-like growth factor binding proteins. Omega-3 and 6 fatty acids such as EPA, DHA and AA has been reported to inhibit MCF-7 cell line growth and proliferation. PIK3CA helical mutations were identified in MCF-7, but with low AKT activation.

References

External links

The University of Texas MD Anderson Breast Cancer Cell Line Data BaseCellosaurus entry for MCF-7
{{DEFAULTSORT:Mcf-7 Human cell lines Breast cancer