M65 or M-65 may refer to:

Military

* Iveco LMV, an Italian-designed four-wheel drive tactical vehicle in service with several countries * M65 atomic cannon, a towed artillery piece * M65 Jacket, a multi-temperate jacket designed for the US Armed forces * M65 recoilless nuclear rifle, a nuclear warhead for recoilless rifles * Soltam M-65, an Israeli mortar * Tikka M65, a Finnish rifle

Transportation

* M65 motorway, a motorway in England * M-65 (Michigan highway), a Michigan state highway * Miles M.65 Gemini, a 1945 British twin-engined four-seat touring aircraft * M65 (Durban), a road in Durban, South Africa

Other uses

* Envelope, size with dimensions 110mm × 220mm * Messier 65, a spiral galaxy in the constellation Leo * M 65, an age group for Masters athletics (athletes aged 35+) {{Letter-NumberCombDisambig