This bacterial growth medium was developed in 1971 for '' Lactococcus'' species isolated from milk products. It was originally called M16 medium, but in 1975 Terzaghi and Sandine added disodium- β-glycerophosphate to the medium as a buffer, and named the new growth medium M17 medium. It was later found that the addition of disodium-β-glycerophosphate inhibits the growth of many ''Lactobacillus'' species.

Typical composition

Per 950 mL: *5.0 g Pancreatic digest of casein *5.0 g Soy Peptone *5.0 g Beef extract *2.5 g

Yeast extract Yeast extracts consist of the cell contents of yeast without the cell walls; they are used as food additives or flavorings, or as nutrients for bacterial culture Growing media, media. They are often used to create savoury flavors and umami tast ...

*0.5 g

Ascorbic acid Ascorbic acid is an organic compound with formula , originally called hexuronic acid. It is a white solid, but impure samples can appear yellowish. It dissolves freely in water to give mildly acidic solutions. It is a mild reducing agent. Asco ...

*0.25 g

Magnesium sulfate Magnesium sulfate or magnesium sulphate is a chemical compound, a salt with the formula , consisting of magnesium cations (20.19% by mass) and sulfate anions . It is a white crystalline solid, soluble in water but not in ethanol. Magnesi ...

*19.0 g Disodium- β-glycerophosphate *11.0 g

Agar Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from " ogonori" and " tengusa". As found in nature, agar is a mixture of two components, t ...

Preparation: *1. Heat with frequent agitation and boil for 1 minute to completely dissolve. *2. Autoclave at 121 °C for 15 minutes. Cool to 50 °C. *3. Add 50 ml filter sterilized 10% lactose solution and mix well (the lactose can be exchanged to other carbohydrates e.g. glucose, resulting in GM17 medium)

References

{{Growth media Microbiological media