The Living Interplanetary Flight ExperimentAsian Scientist
"Phobos-Grunt Mission Carrying Yinghuo-1 Space Probe Suffers Technical Glitch"
Srinivas Laxman, 9 November 2011 (LIFE or Phobos LIFE) was an interplanetary mission developed by the Planetary Society. It consisted of sending selected microorganisms on a three-year interplanetary round-trip in a small capsule aboard the Russian

Fobos-Grunt Fobos-Grunt or Phobos-Grunt () was an attempted Russian sample return mission to Phobos (moon), Phobos, one of the moons of Mars. Fobos-Grunt also carried the Chinese Mars orbiter Yinghuo-1 and the tiny Living Interplanetary Flight Experiment f ...

spacecraft in 2011, which was a failed sample-return mission to the Martian moon Phobos. The Fobos-Grunt mission failed to leave Earth orbitUniverse Today
"Russian Space Program Prepares for Phobos-Grunt Re-Entry"
David Warmflash, 13 December 2011 and was destroyed. The goal was to test whether selected

organism An organism is any life, living thing that functions as an individual. Such a definition raises more problems than it solves, not least because the concept of an individual is also difficult. Many criteria, few of them widely accepted, have be ...

s can survive an undetermined number of years in deep space by flying them through interplanetary space. The experiment would have tested one aspect of

panspermia Panspermia () is the hypothesis that life exists throughout the universe, distributed by space dust, meteoroids, asteroids, comets, and planetoids, as well as by spacecraft carrying unintended contamination by microorganisms,Forward planetary c ...

, the hypothesis that

life Life, also known as biota, refers to matter that has biological processes, such as Cell signaling, signaling and self-sustaining processes. It is defined descriptively by the capacity for homeostasis, Structure#Biological, organisation, met ...

could survive space travel, if protected inside rocks blasted by impact off one planet to land on another.

Precursor

Prior to the ''Phobos LIFE'' experiment, a precursor ''LIFE'' prototype was successfully flown in 2011 aboard the final flight of

Space Shuttle Endeavour Space Shuttle ''Endeavour'' (Orbiter Vehicle Designation: OV-105) is a retired Space Shuttle orbiter, orbiter from NASA's Space Shuttle program and the fifth and final operational Space Shuttle, Shuttle built. It embarked on its first mission, ...

STS-134 STS-134 (ISS assembly sequence, ISS assembly flight ULF6) was the penultimate mission of NASA's Space Shuttle program and the 25th and last spaceflight of . This flight delivered the Alpha Magnetic Spectrometer and an ExPRESS Logistics Carrier ...

. Known as the Shuttle-LIFE (also LIFE) experiment.SPACE.com
"Salvaging Science from Stricken Mars Moon Probe: A Scientist's View"
David Warmflash, 11 November 2011

The experiment

The project includes representatives of all

three domains of life The three-domain system is a taxonomic classification system that groups all cellular life into three domains, namely Archaea, Bacteria and Eukarya, introduced by Carl Woese, Otto Kandler and Mark Wheelis in 1990. The key difference from earli ...

bacteria Bacteria (; : bacterium) are ubiquitous, mostly free-living organisms often consisting of one Cell (biology), biological cell. They constitute a large domain (biology), domain of Prokaryote, prokaryotic microorganisms. Typically a few micr ...

eukaryota The eukaryotes ( ) constitute the Domain (biology), domain of Eukaryota or Eukarya, organisms whose Cell (biology), cells have a membrane-bound cell nucleus, nucleus. All animals, plants, Fungus, fungi, seaweeds, and many unicellular organisms ...

and

archaea Archaea ( ) is a Domain (biology), domain of organisms. Traditionally, Archaea only included its Prokaryote, prokaryotic members, but this has since been found to be paraphyletic, as eukaryotes are known to have evolved from archaea. Even thou ...

. The capsule was transporting 10 types of organisms in 30 self-contained samples, i.e., each in triplicate. In addition, one or more natural native soil samples were flown in their own self-contained capsule. The Phobos-Soil sample return mission was the only attempted biological science mission that would have returned to Earth from deep space, far beyond the protection of Earth's magnetic field; sending biological samples through deep space is therefore a much better test of interplanetary survivability than sending the samples on a typical Earth-orbiting flight. The project was being done in collaboration with the Russian Space Research Institute, the Institute for Biomedical Problems of the

Russian Academy of Sciences The Russian Academy of Sciences (RAS; ''Rossíyskaya akadémiya naúk'') consists of the national academy of Russia; a network of scientific research institutes from across the Russian Federation; and additional scientific and social units such ...

, the

Moscow State University Moscow State University (MSU), officially M. V. Lomonosov Moscow State University,. is a public university, public research university in Moscow, Russia. The university includes 15 research institutes, 43 faculties, more than 300 departments, a ...

, the

American Type Culture Collection ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development. Established in 1925 to serve as a nati ...

(ATCC), and the Institute for Aerospace Medicine in Germany.

Specimens

Three fundamental guidelines governed the selection of the organisms: First, the organisms selected represent the

–

eukaryote The eukaryotes ( ) constitute the Domain (biology), domain of Eukaryota or Eukarya, organisms whose Cell (biology), cells have a membrane-bound cell nucleus, nucleus. All animals, plants, Fungus, fungi, seaweeds, and many unicellular organisms ...

and

. Second, the organisms are very well studied (e.g., having their

genome A genome is all the genetic information of an organism. It consists of nucleotide sequences of DNA (or RNA in RNA viruses). The nuclear genome includes protein-coding genes and non-coding genes, other functional regions of the genome such as ...

sequenced and studied in many other experiments) to make it possible to accurately assess the effects of the long exposure to space. If they had already been studied in space conditions so much the better, since it would enable researchers to pinpoint precisely how organisms were affected by the years-long exposure to the interplanetary environment. Finally, a strong preference was given to organisms that appear to stand the best chance of surviving the journey. These are

extremophile An extremophile () is an organism that is able to live (or in some cases thrive) in extreme environments, i.e., environments with conditions approaching or stretching the limits of what known life can adapt to, such as extreme temperature, press ...

s, organisms that thrive in conditions that would kill the vast majority of Earthly creatures. The 10 'passenger' organisms selected are listed below:

Bacteria Bacteria (; : bacterium) are ubiquitous, mostly free-living organisms often consisting of one Cell (biology), biological cell. They constitute a large domain (biology), domain of Prokaryote, prokaryotic microorganisms. Typically a few micr ...

*'' Bacillus safensis'' **Discovered in JPL's 'clean' room: Spacecraft Assembly Facility.The Planetary Report, Volume XXIX, number 2, March/April 2009, "We make it happen! Who will survive? Ten hardy organisms selected for the LIFE project, by Amir Alexander https://www.planetary.org/planetary-report/tpr-2009-2 Might already be on Mars with Spirit and

Opportunity Opportunity may refer to: Places * Opportunity, Montana, an unincorporated community, United States * Opportunity, Nebraska, an unincorporated community, United States * Opportunity, Washington, a former census-designated place, United States * ...

. *'' Deinococcus radiodurans'' **Is extremely resistant to radiation, can survive a dose of 5,000 Gy. *''

Bacillus subtilis ''Bacillus subtilis'' (), known also as the hay bacillus or grass bacillus, is a gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants, humans and marine sponges. As a member of the genus ''Bacill ...

'' ** strain 168: common strain used in biology. ** strain MW01: UV-resistant strain evolved from 168. Part of EXPOSE-E. ** This species is well known from other astrobiological experiments. It has flown to the Moon with Apollo and had multiyear exposure in

low Earth orbit A low Earth orbit (LEO) is an geocentric orbit, orbit around Earth with a orbital period, period of 128 minutes or less (making at least 11.25 orbits per day) and an orbital eccentricity, eccentricity less than 0.25. Most of the artificial object ...

Archaea Archaea ( ) is a Domain (biology), domain of organisms. Traditionally, Archaea only included its Prokaryote, prokaryotic members, but this has since been found to be paraphyletic, as eukaryotes are known to have evolved from archaea. Even thou ...

*'' Haloarcula marismortui'' **If Mars had an ocean, it would have been very salty. ''H. marismortui'' is

halophilic A halophile (from the Greek word for 'salt-loving') is an extremophile that thrives in high salt concentrations. In chemical terms, halophile refers to a Lewis acidic species that has some ability to extract halides from other chemical species. ...

. *'' Methanothermobacter wolfeii'' **

Mars Express ''Mars Express'' is a space exploration mission by the European Space Agency, European Space Agency (ESA) exploring the planet Mars and its moons since 2003, and the first planetary mission attempted by ESA. ''Mars Express'' consisted of two ...

has discovered methane in the Martian atmosphere. ''M. wolfeii'' is a methane-producing organism. *'' Pyrococcus furiosus'' **''P. furiosus'' thrives at about 100 °C, it was supposed to act as a maximum temperature indicator.

Eukaryote The eukaryotes ( ) constitute the Domain (biology), domain of Eukaryota or Eukarya, organisms whose Cell (biology), cells have a membrane-bound cell nucleus, nucleus. All animals, plants, Fungus, fungi, seaweeds, and many unicellular organisms ...

Fungus A fungus (: fungi , , , or ; or funguses) is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and mold (fungus), molds, as well as the more familiar mushrooms. These organisms are classified as one ...

– ''

Saccharomyces cerevisiae ''Saccharomyces cerevisiae'' () (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungal microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have be ...

'' (yeast) *

Plant Plants are the eukaryotes that form the Kingdom (biology), kingdom Plantae; they are predominantly Photosynthesis, photosynthetic. This means that they obtain their energy from sunlight, using chloroplasts derived from endosymbiosis with c ...

ae – Seeds from ''

Arabidopsis thaliana ''Arabidopsis thaliana'', the thale cress, mouse-ear cress or arabidopsis, is a small plant from the mustard family (Brassicaceae), native to Eurasia and Africa. Commonly found along the shoulders of roads and in disturbed land, it is generally ...

'' ('mouse-ear cress') **Flew to the Moon with Apollo. *

Animal Animals are multicellular, eukaryotic organisms in the Biology, biological Kingdom (biology), kingdom Animalia (). With few exceptions, animals heterotroph, consume organic material, Cellular respiration#Aerobic respiration, breathe oxygen, ...

ia –

Tardigrade Tardigrades (), known colloquially as water bears or moss piglets, are a phylum of eight-legged segmented micro-animals. They were first described by the German zoologist Johann August Ephraim Goeze in 1773, who called them . In 1776, th ...

s ('water bears') **Have survived vacuum and radiation in low Earth orbit.

Capsule design

The mass of the Bio-Module on board the Fobos-Grunt spacecraft was 100 grams or less. The design is a short

cylinder A cylinder () has traditionally been a three-dimensional solid, one of the most basic of curvilinear geometric shapes. In elementary geometry, it is considered a prism with a circle as its base. A cylinder may also be defined as an infinite ...

. The bio-module provided 30 small tubes (3 millimeters in diameter) for individual microbe samples. It also accommodated a native sample of bacteria – derived from a

permafrost Permafrost () is soil or underwater sediment which continuously remains below for two years or more; the oldest permafrost has been continuously frozen for around 700,000 years. Whilst the shallowest permafrost has a vertical extent of below ...

region on

Earth Earth is the third planet from the Sun and the only astronomical object known to Planetary habitability, harbor life. This is enabled by Earth being an ocean world, the only one in the Solar System sustaining liquid surface water. Almost all ...

– within a cavity 26 mm in diameter.

Mission failure

The module passed stress tests including a shake test with vibrations at frequencies to 1,100 Hz and an impact test of 4,000 g, designed to simulate the potential impact of the capsule on Earth. The LIFE experiment was launched on November 8, 2011 on board the

. However, the spacecraft failed to depart Earth orbit due to a programming error, and fell back to Earth in the Pacific Ocean.RIA Novosti
"Phobos-Grunt mission ‘impossible,’ says chief designer"
13 December 2011 The module was not recovered. The team is seeking out future exploratory opportunities.

References

External links

* The Planetary Society
Shuttle LIFE
* The Planetary Society
Phobos LIFE
* The Planetary Society
LIFE
{{astrobiology Fobos-Grunt Space science experiments Cancelled astrobiology space missions The Planetary Society Life in outer space