In public transport, Route 16 may refer to:
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Route 16 (MTA Maryland)
LocalLink 29 is a bus route operated by the Maryland Transit Administration in Baltimore. The line currently runs from the Mondawmin Metro Subway Station in Northwest Baltimore to Brooklyn Homes in South Baltimore through the communities of Ro ...
, a bus route in Baltimore, Maryland and its suburbs
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London Buses route 16
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Melbourne tram route 16
Melbourne tram route 16 is a tram route on the Melbourne tramway network serving the city of Melbourne in Victoria, Australia. Operated by Yarra Trams, the route is coloured light yellow and extends from Melbourne University to Kew over 20. ...
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16