LAPC4 cells are a
cell line
An immortalised cell line is a population of cells from a multicellular organism that would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells ...
of human
prostate cancer
Prostate cancer is the neoplasm, uncontrolled growth of cells in the prostate, a gland in the male reproductive system below the bladder. Abnormal growth of the prostate tissue is usually detected through Screening (medicine), screening tests, ...
commonly used in the field of
oncology
Oncology is a branch of medicine that deals with the study, treatment, diagnosis, and prevention of cancer. A medical professional who practices oncology is an ''oncologist''. The name's Etymology, etymological origin is the Greek word ὄγ ...
. The tissue was harvested from the lymph node metastasis of a male patient with hormone refractory prostate cancer which was then xenografted into SCID mice and later harvested and plated on tissue culture dishes, where it can be propagated as an immortalized prostate cancer cell line.
Characteristics
LAPC4 are a lowly adherent, epithelial cell line with high
Androgen receptor
The androgen receptor (AR), also known as NR3C4 (nuclear receptor subfamily 3, group C, member 4), is a type of nuclear receptor that is activated by binding any of the androgenic hormones, including testosterone and dihydrotestosterone, in th ...
and
Prostate specific antigen
The prostate is an accessory gland of the male reproductive system and a muscle-driven mechanical switch between urination and ejaculation. It is found in all male mammals. It differs between species anatomically, chemically, and physiological ...
expression.
Unlike the other commonly utilized, Androgen receptor positive prostate cancer cell lines
LNCaP
LNCaP cells are a cell line of human cells commonly used in the field of oncology. LNCaP cells are androgen-sensitive human prostate adenocarcinoma cells derived from the left supraclavicular lymph node metastasis from a 50-year-old caucasian ma ...
and
VCaP
VCaP cells are a cell line of human prostate cancer commonly used in the field of oncology. The tissue was harvested at autopsy from a metastatic lesion to a lumbar vertebrae of a 59 year old Caucasian male with hormone refractory prostate cancer i ...
, LAPC4 have high expression of
Keratin 5
Keratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the ''KRT5'' gene. It protein dimer, dimerizes with keratin 14 and forms the intermediate filaments (IF) that make up the cytoskeleton of Stratum basale, basal ...
, a basal marker, as well as the luminal markers
Keratin 8
Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the ''KRT8'' gene. It is often paired with keratin 18.
Utility as an immunohistochemical stain
Antibodies to ...
and
Keratin 18
Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutatio ...
.
[ LAPC4 also expresses mutated P53 (R175H). ]
References
External links
Cellosaurus entry for LAPC4
Human cell lines
Prostate cancer
{{oncology-stub