The Knife-Edge Scanning Microscope (KESM) was invented and

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ed in the late 1990s by Bruce McCormick at

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. The microscope is intended to produce

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data sets in order to reconstruct 3D cellular structures. The machine is capable of handling tissue volumes of 1 to 100mm³, recording large volumes of tissue in a small amount of time (~7mm²s⁻¹). The resolution and scanning speed of KESM is a novel method for imaging tissue at resolutions sufficient to reconstruct maps of cellular distribution and

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. The technique preserves the alignment of serial sections accurately enough to reconstruct

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al processes and microvasculature.Knife-edge scanning microscope (KESM): http://research.cs.tamu.edu/bnl/kesm.html

References

Microscopes