Indirect immunoperoxidase assay (IPA) is a laboratory technique used to detect and

titrate Titration (also known as titrimetry and volumetric analysis) is a common laboratory method of Quantitative research, quantitative Analytical chemistry, chemical analysis to determine the concentration of an identified analyte (a substance to be ...

viruses that do not cause measurable

cytopathic effect Cytopathic effect (abbreviated CPE) refers to structural changes in host cells that are caused by viral invasion. The infecting virus causes lysis of the host cell or when the cell dies without lysis due to an inability to replicate. If a virus c ...

s and cannot be measured by classical plaque assays. These viruses include human coronavirus 229E and OC43.

Methodology

Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96-well plate. After viral growth, viral detection by IPA yields the infectious virus titer, expressed as tissue culture infectious dose (TCID50). This represents the dilution of a virus-containing sample at which half of a series of laboratory wells contain replicating viruses. This technique is a reliable method for the titration of human coronaviruses (HCoV) in biological samples (cells, tissues, or fluids). It is also reliable in the detection of antibodies to

human cytomegalovirus Human cytomegalovirus (HCMV), also called human herpesvirus 5 (HHV-5), is a species of virus in the genus ''Cytomegalovirus'', which in turn is a member of the viral family known as ''Herpesviridae'' or herpesviruses. It is also commonly call ...

.G Gerna, C J McCloud and R W Chambers: Immunoperoxidase technique for detection of antibodies to Human cytomegalovirus. J. Clin. Microbiol. 1976, 3(3):364.

References

External links

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Immunohistochemistry Protocols, Buffers and Troubleshooting
{{Immunologic techniques and tests, state expand Biochemistry methods Diagnostic virology Immunologic tests Laboratory techniques

Methodology

See also

References

External links