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Heller's test is a
chemical test In chemistry, a chemical test is a qualitative or quantitative procedure designed to identify, quantify, or characterise a chemical compound or chemical group. Purposes Chemical testing might have a variety of purposes, such as to: * Determine ...
that shows that
strong acid Acid strength is the tendency of an acid, symbolised by the chemical formula HA, to dissociate into a proton, H+, and an anion, A-. The dissociation of a strong acid in solution is effectively complete, except in its most concentrated solutio ...
s cause the denaturation of precipitated proteins. Concentrated
nitric acid Nitric acid is the inorganic compound with the formula . It is a highly corrosive mineral acid. The compound is colorless, but older samples tend to be yellow cast due to decomposition into oxides of nitrogen. Most commercially available ni ...
is added to a protein solution from the side of the test tube to form two layers. A white ring appears between the two layers if the test is positive. Heller's test is commonly used to test for the presence of proteins in
urine Urine is a liquid by-product of metabolism in humans and in many other animals. Urine flows from the kidneys through the ureters to the urinary bladder. Urination results in urine being excreted from the body through the urethra. Cellular ...
. This test was discovered by the Austrian Chemist, Johann Florian Heller (1813-1871).


References

Chemical tests {{analytical-chemistry-stub