HIS-selective medium is a type
cell culture medium that lacks the
amino acid histidine. It can be used with bacteria reliant on the expression of a gene encoding proteins involved in histidine expression in order to survive.
[Joung, J. K., E. I. Ramm and C. O. Pabo (2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions." Proc Natl Acad Sci U S A 97(13): 7382-7.] Only bacteria expressing such genes (such as
hisB in ''
Escherichia coli'' and
HIS3 in ''
Saccharomyces cerevisiae'') will survive on these media.
References
Cell culture media
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