The HBV RNA encapsidation signal epsilon
HBV_epsilon is an element essential for
HBV virus replication.
It is an
RNA structure
Nucleic acid structure refers to the structure of nucleic acids such as DNA and RNA. Chemically speaking, DNA and RNA are very similar. Nucleic acid structure is often divided into four different levels: primary, secondary, tertiary, and quaterna ...
situated near the
5' end of the
HBV pregenomic RNA.
The structure consists of a lower stem, a bulge region, an upper stem and a tri-loop.
The structure was determined and refined through enzymatic probing and
NMR spectroscopy
Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique based on re-orientation of atomic nuclei with non-zero nuclear spins in an external magnetic f ...
.
The closure of the tri-loop was not predicted by
RNA structure prediction programs but observed in the NMR structure.
The regions shown to be critical for encapsidation of the RNA in the viral lifecycle are the bulge, upper stem and tri-loop which interact with the terminal protein domain of the HBV viral polymerase.
See also
*
Heron HBV RNA encapsidation signal epsilon
*
Duck HBV RNA encapsidation signal epsilon
*
Hepatitis B virus PRE alpha
*
Hepatitis B virus PRE beta
*
Hepatitis B virus PRE 1151–1410
References
External links
*
HBVRegDB Hepatitis B Virus HBV Regulatory Sequence Database (HBVRegDB)
Cis-regulatory RNA elements
Hepatitis B virus
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