The HBV RNA encapsidation signal epsilon
HBV_epsilon is an element essential for
HBV virus replication.
It is an
RNA structure situated near the
5' end of the
HBV pregenomic RNA.
The structure consists of a lower stem, a bulge region, an upper stem and a tri-loop.
The structure was determined and refined through enzymatic probing and
NMR spectroscopy
Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique to observe local magnetic fields around atomic nuclei. The sample is placed in a magnetic fiel ...
.
The closure of the tri-loop was not predicted by
RNA structure prediction
Nucleic acid structure prediction is a computational method to determine ''secondary'' and ''tertiary'' nucleic acid structure from its sequence. Secondary structure can be predicted from one or several nucleic acid sequences. Tertiary structur ...
programs but observed in the NMR structure.
The regions shown to be critical for encapsidation of the RNA in the viral lifecycle are the bulge, upper stem and tri-loop which interact with the terminal protein domain of the HBV viral polymerase.
See also
*
Heron HBV RNA encapsidation signal epsilon
The Avian HBV RNA encapsidation signal epsilon (AHBV epsilon) is an RNA structure that is shown to facilitate encapsidation of the pregenomic RNA required for viral replication. There are two main classes of encapsidation signals in avian he ...
*
Duck HBV RNA encapsidation signal epsilon
*
Hepatitis B virus PRE alpha
*
Hepatitis B virus PRE beta
*
Hepatitis B virus PRE 1151–1410
References
External links
*
HBVRegDB Hepatitis B Virus HBV Regulatory Sequence Database (HBVRegDB)
Cis-regulatory RNA elements
Hepatitis B virus
{{molecular-cell-biology-stub