''Geobacillus thermoglucosidasius'' is a

thermophilic A thermophile is a type of extremophile that thrives at relatively high temperatures, between . Many thermophiles are archaea, though some of them are bacteria and fungi. Thermophilic eubacteria are suggested to have been among the earliest bact ...

gram-positive In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is ...

bacterium Bacteria (; : bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the ...

, and a member of the ''

Bacillota The Bacillota (synonym Firmicutes) are a phylum of bacteria, most of which have Gram-positive cell wall structure. They have round cells, called cocci (singular coccus), or rod-like forms (bacillus). A few Bacillota, such as '' Megasphaera'', ...

'' phylum. It was first isolated from soil in Japan in 1983. The species name ''thermoglucosidasius'' comes from the words ''therme'' denoting heat, and ''glucosidasius'' denoting starch-hydrolyzing glucosidase activity.

Biology and biochemistry

''G. thermoglucosidasius'' is

(bacterium that retains

Crystal violet Crystal violet or gentian violet, also known as methyl violet 10B or hexamethyl pararosaniline chloride, is a triphenylmethane, triarylmethane dye used as a histological stain and in Gram staining, Gram's method of classifying bacteria. Crystal ...

dye during gram-staining) and

facultatively anaerobic A facultative anaerobic organism is an organism that makes ATP by aerobic respiration if oxygen is present, but is capable of switching to fermentation if oxygen is absent. Some examples of facultatively anaerobic bacteria are ''Staphylococcus' ...

(produces ATP by

aerobic respiration Cellular respiration is the process of oxidizing biological fuels using an inorganic electron acceptor, such as oxygen, to drive production of adenosine triphosphate (ATP), which stores chemical energy in a biologically accessible form. Cellu ...

oxygen Oxygen is a chemical element; it has chemical symbol, symbol O and atomic number 8. It is a member of the chalcogen group (periodic table), group in the periodic table, a highly reactivity (chemistry), reactive nonmetal (chemistry), non ...

is present, but capable of switching to

fermentation Fermentation is a type of anaerobic metabolism which harnesses the redox potential of the reactants to make adenosine triphosphate (ATP) and organic end products. Organic molecules, such as glucose or other sugars, are catabolized and reduce ...

anaerobic respiration Anaerobic respiration is respiration using electron acceptors other than molecular oxygen (O2). Although oxygen is not the final electron acceptor, the process still uses a respiratory electron transport chain. In aerobic organisms undergoing ...

if oxygen is absent). ''G. thermoglucosidasius'' is classified as a thermophile as optimal growth occurs at 60 °C (140 °F), although strains have demonstrated ability to grow at temperatures between 37 °C (98.6 °F) and 68 °C (154.4 °F). Their rod-shaped cells are less than 3.0

micrometers The micrometre (Commonwealth English as used by the International Bureau of Weights and Measures; SI symbol: μm) or micrometer (American English), also commonly known by the non-SI term micron, is a unit of length in the International System ...

(μm) long and less than 0.9 μm in diameter. Under a microscope, the cells are observed to occur either singly or in short chains, while possessing peritrichous fagella for motility or appearing non-motile. Vegetative ''G. thermoglucosidasius'' sporulates, producing one endospore per cell located terminally or subterminally in slightly swollen or non-swollen sporangia. It can live on a wide variety of substrates. ''G. thermoglucosidasius'' uses mixed-acid fermentation in anaerobic conditions, producing lactate,

succinate Succinic acid () is a dicarboxylic acid with the chemical formula (CH2)2(CO2H)2. In living organisms, succinic acid takes the form of an anion, succinate, which has multiple biological roles as a metabolic intermediate being converted into Fuma ...

formate Formate (IUPAC name: methanoate) is the conjugate base of formic acid. Formate is an anion () or its derivatives such as ester of formic acid. The salts and esters are generally colorless. Fundamentals When dissolved in water, formic acid co ...

ethanol Ethanol (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound with the chemical formula . It is an Alcohol (chemistry), alcohol, with its formula also written as , or EtOH, where Et is the ps ...

acetate An acetate is a salt formed by the combination of acetic acid with a base (e.g. alkaline, earthy, metallic, nonmetallic, or radical base). "Acetate" also describes the conjugate base or ion (specifically, the negatively charged ion called ...

and

carbon dioxide Carbon dioxide is a chemical compound with the chemical formula . It is made up of molecules that each have one carbon atom covalent bond, covalently double bonded to two oxygen atoms. It is found in a gas state at room temperature and at norma ...

. Growth can be driven by

aerobic Aerobic means "requiring air," in which "air" usually means oxygen. Aerobic may also refer to * Aerobic exercise, prolonged exercise of moderate intensity * Aerobics, a form of aerobic exercise * Aerobic respiration, the aerobic process of cellu ...

, using a large variety of redox pairs.

Taxonomy

Prior to 1997, ''G. thermoglucosidasius'' was categorized into the

genus Genus (; : genera ) is a taxonomic rank above species and below family (taxonomy), family as used in the biological classification of extant taxon, living and fossil organisms as well as Virus classification#ICTV classification, viruses. In bino ...

Bacillus ''Bacillus'', from Latin "bacillus", meaning "little staff, wand", is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum ''Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-sh ...

'' in Group 5, a phenotypically and phylogenetically coherent group of

bacilli Bacilli is a Taxonomy (biology), taxonomic Class (biology), class of bacteria that includes two orders, Bacillales and Lactobacillales, which contain several well-known pathogens such as ''Bacillus anthracis'' (the cause of anthrax). ''Bacilli'' ...

displaying very high similarity among their

16S rRNA 16S ribosomal RNA (or 16Svedberg, S rRNA) is the RNA component of the 30S subunit of a prokaryotic ribosome (SSU rRNA). It binds to the Shine-Dalgarno sequence and provides most of the SSU structure. The genes coding for it are referred to as ...

sequences. However, on the basis of physiological characteristics, fatty acid analysis, DNA hybridization studies and 16S rRNA gene sequence analysis, Nazina ''et al.'' proposed the creation of the genus ''Geobacillus'' to contain ''B. thermoglucosidasius'', ''B. stearothermophilus'' (type species), ''B. thermoleovorans'', ''B. thermocatenulatus'', ''B. kaustophilus'', and ''B. thermodenitricans''. The type strain of ''G. thermoglucosidasius'' was subsequently chosen as strain DSM....

Genome

To date, three completed public genome sequences are accessible. ...

Metabolism

Most thermoglucosidasius strains have hydrolytic activity to starch, gelatin, and pullulan, as well as producing acid from adonitol, cellobiose, inositol, and D-xylitol. Colonies are offwhite and mucoid.

Use in Biotechnology

''G. thermoglucosidasius'' is the source of the enzyme BtgZI, a type IIS

Restriction enzyme A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class o ...

used in

Golden Gate Cloning Golden Gate Cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Restriction enzyme#Type II, Type IIS restriction enz ...

.https://www.neb.com/en-us/products/r0703-btgzi

References

External links

Type strain of ''Geobacillus thermoglucosidasius'' at Bac''Dive'' - the Bacterial Diversity Metadatabase
{{Taxonbar, from=Q5533950 Thermophiles Bacillaceae