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A gate in
"General Flow Cytometry Glossary and Cell Cycle Analysis Terminology"
The Janis V. Giorgi Flow Cytometry Laboratory, UCLA
{{DEFAULTSORT:Gate (Cytometry) Flow cytometry Technology systems
A gate in cytometry
Cytometry is the measurement of number and characteristics of cells. Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or a ...
is a set of value limits (boundaries) that serve to isolate a specific group of cytometric events from a large set. Gates can be defined by discrimination analysis, or can simply be drawn around a given set of data points on a printout and then converted to a computer-useful form. Gates can be implemented with a physical blinder. Gates may be used either to selectively gather data or to segregate data for analysis.
Division
Gates are divided mathematically into inclusive gates and exclusive gates. Inclusive gates select data that falls within the limits set, while exclusive gates select data that falls outside the limits.Live gate
A live gate is a term used for a process that prevents the acquisition by the computer of non-selected data from theflow cytometer
Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles.
In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow ...
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External links
"General Flow Cytometry Glossary and Cell Cycle Analysis Terminology"
The Janis V. Giorgi Flow Cytometry Laboratory, UCLA
{{DEFAULTSORT:Gate (Cytometry) Flow cytometry Technology systems