The FluoProbes series of fluorescent dyes were developed by Interchim to improve performances of standard fluorophores. They are designed for labeling

biomolecule A biomolecule or biological molecule is loosely defined as a molecule produced by a living organism and essential to one or more typically biological processes. Biomolecules include large macromolecules such as proteins, carbohydrates, lipids ...

s, cells, tissues or beads in advanced fluorescent detection techniques. * FluoProbes dyes are typically used to label proteins or nucleic acids (ultrafast -3 minutes- labeling of

antibodies An antibody (Ab) or immunoglobulin (Ig) is a large, Y-shaped protein belonging to the immunoglobulin superfamily which is used by the immune system to identify and neutralize antigens such as bacteria and viruses, including those that caus ...

employs Lightning technology). Labeled products can be used for multiparameter detections, life time resolved fluorescence (TRF), polarisation anisotropy fluorescence, FRET, Quenching, FRAP. They are typically used in biotechnology and research applications as fluorescence microscopy,

cell biology Cell biology (also cellular biology or cytology) is a branch of biology that studies the structure, function, and behavior of cells. All living organisms are made of cells. A cell is the basic unit of life that is responsible for the living an ...

molecular biology Molecular biology is a branch of biology that seeks to understand the molecule, molecular basis of biological activity in and between Cell (biology), cells, including biomolecule, biomolecular synthesis, modification, mechanisms, and interactio ...

, as well as infrared imaging. Derivatives with

Amine In chemistry, amines (, ) are organic compounds that contain carbon-nitrogen bonds. Amines are formed when one or more hydrogen atoms in ammonia are replaced by alkyl or aryl groups. The nitrogen atom in an amine possesses a lone pair of elec ...

and Carboxyl suit peptide and nucleic acid synthesis, while reactive ones (with succinimidyl, maleimide and hydrazide) suit conjugation by conventional chemistry, while FluoProbes labeled antibodies and cellular probes (i.e. Phalloidin) suit direct use in immunoassays or cell assays. * FluoProbes dyes that have comparable excitation and emission spectra to standard fluorophores such as fluoresceins, rhodamines, cyanines Cy2/3/5/5.5/7, are claimed to solve limiting issues observed in some applications such as too high background, insufficient polarity,

photobleaching In optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused by cleaving of covalent bonds or non-specific reactions between ...

, insufficient brightness, or pH-sensitivity. I.e., FluoProbes488 reduces the background in Flow Cytometry and in slide microscopy allowing sharper and brighter images. FluoProbes 488, 547H and 647H are found more photostable that is taken to good account in applications using long illuminations periods (i.e. scanning such as in

confocal microscopy Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast (vision), contrast of a micrograph by me ...

),FluoProbes547H comparison in Confocal Microscopy
/ref> or for longer shelf-life of reagents (i.e. manufacturing diagnostics). * The

excitation Excitation, excite, exciting, or excitement may refer to: * Excitation (magnetic), provided with an electrical generator or alternator * ''Exite'', a series of racing video games published by Nintendo starting with ''Excitebike'' * Excite (web port ...

and

emission Emission may refer to: Chemical products * Emission of air pollutants, notably: ** Flue gas, gas exiting to the atmosphere via a flue ** Exhaust gas, flue gas generated by fuel combustion ** Emission of greenhouse gases, which absorb and emit rad ...

spectra of the FluoProbes series covers much of the

visible spectrum The visible spectrum is the spectral band, band of the electromagnetic spectrum that is visual perception, visible to the human eye. Electromagnetic radiation in this range of wavelengths is called ''visible light'' (or simply light). The optica ...

up to the

infrared Infrared (IR; sometimes called infrared light) is electromagnetic radiation (EMR) with wavelengths longer than that of visible light but shorter than microwaves. The infrared spectral band begins with the waves that are just longer than those ...

region, matching the commonly used light sources and filters. Similar lines of fluorescent dyes provide an alternative to the FluoProbes Dyes.

References

{{DEFAULTSORT:Fluoprobes Dyes Fluorescent dyes