Eijkman test, or differential coliform test,
or confirmed Escherichia coli count,
is a test used for the identification of
coliform bacteria
Coliform bacteria are defined as either motile or non-motile Gram-negative non- spore forming Bacilli that possess β-galactosidase to produce acids and gases under their optimal growth temperature of 35-37°C. They can be aerobes or faculta ...
from
warm-blooded animals
Warm-blooded is an informal term referring to animal species which can maintain a body temperature higher than their environment. In particular, homeothermic species maintain a stable body temperature by regulating metabolic processes. The onl ...
based on the bacteria's ability to produce gas when grown in glucose media at 46°C (114.8°F).
The test to determine whether coliform bacteria come from warm-blooded animals. By means of this test it can be readily established if
water
Water (chemical formula ) is an inorganic, transparent, tasteless, odorless, and nearly colorless chemical substance, which is the main constituent of Earth's hydrosphere and the fluids of all known living organisms (in which it acts as ...
has been polluted by human and animal
defecation
Defecation (or defaecation) follows digestion, and is a necessary process by which organisms eliminate a solid, semisolid, or liquid waste material known as feces from the digestive tract via the anus. The act has a variety of names ranging ...
containing
coli bacilli
''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Esch ...
.
The test was introduced by
Christiaan Eijkman
Christiaan Eijkman ( , , ; 11 August 1858 – 5 November 1930) was a Dutch physician and professor of physiology whose demonstration that beriberi is caused by poor diet led to the discovery of antineuritic vitamins ( thiamine). Together w ...
(1858–1930) in his paper in 1904.
References
{{Med-diagnostic-stub
Medical tests