''Cryptocalicium'' is a single-species fungal

genus Genus (; : genera ) is a taxonomic rank above species and below family (taxonomy), family as used in the biological classification of extant taxon, living and fossil organisms as well as Virus classification#ICTV classification, viruses. In bino ...

of the order

Eurotiomycetes Eurotiomycetes is a large class of ascomycetes with cleistothecial ascocarps within the subphylum Pezizomycotina, currently containing around 3810 species according to the Catalogue of Life. It is the third largest lichenized class, with more th ...

that was

circumscribed In geometry, a circumscribed circle for a set of points is a circle passing through each of them. Such a circle is said to ''circumscribe'' the points or a polygon formed from them; such a polygon is said to be ''inscribed'' in the circle. * Circum ...

in 2021. It contains the species ''Cryptocalicium blascoi''. ''Cryptocalicium'' is the only genus in the monotypic

family Family (from ) is a Social group, group of people related either by consanguinity (by recognized birth) or Affinity (law), affinity (by marriage or other relationship). It forms the basis for social order. Ideally, families offer predictabili ...

Cryptocaliciaceae, the

order Order, ORDER or Orders may refer to: * A socio-political or established or existing order, e.g. World order, Ancien Regime, Pax Britannica * Categorization, the process in which ideas and objects are recognized, differentiated, and understood ...

Cryptocaliciales and the subclass Cryptocaliciomycetidae. ''Cryptocalicium'' is a representative of the "mazaediate" or "calicioid" fungi, a

polyphyletic A polyphyletic group is an assemblage that includes organisms with mixed evolutionary origin but does not include their most recent common ancestor. The term is often applied to groups that share similar features known as Homoplasy, homoplasies ...

group of independently evolved

ascomycetes Ascomycota is a phylum of the kingdom Fungi that, together with the Basidiomycota, forms the subkingdom Dikarya. Its members are commonly known as the sac fungi or ascomycetes. It is the largest phylum of Fungi, with over 64,000 species. The de ...

that share a morphological characteristic: the presence of a distinct structure in which loose masses of

ascospores In fungi, an ascospore is the sexual spore formed inside an ascus—the sac-like cell that defines the division Ascomycota, the largest and most diverse division of fungi. After two parental nuclei fuse, the ascus undergoes meiosis (halving of ...

accumulate to be passively disseminated, called the

mazaedium This glossary provides an overview of scientific terminology, terms used in the description of lichens, composite organisms arising from algae or cyanobacteria living symbiosis, symbiotically among Hypha, filaments of multiple fungus species. Er ...

Morphology

Members of ''Cryptocalicium'' have very small stalked apothecioid ascomata that produce a

. The

hymenium The hymenium is the tissue layer on the hymenophore of a fungal fruiting body where the cells develop into basidia or asci, which produce spores. In some species all of the cells of the hymenium develop into basidia or asci, while in oth ...

of the

ascomata An ascocarp, or ascoma (: ascomata), is the fruiting body (sporocarp (fungi), sporocarp) of an ascomycete phylum fungus. It consists of very tightly interwoven hyphae and millions of embedded ascus, asci, each of which typically contains four to ...

presents septate, sterile (i.e. not

fertile Fertility in colloquial terms refers the ability to have offspring. In demographic contexts, fertility refers to the actual production of offspring, rather than the physical capability to reproduce, which is termed fecundity. The fertility rate is ...

) protruding elements. Their asci are clavate in shape with a long stalk, they are bitunicate with evanescent walls, and turn

amyloid Amyloids are aggregates of proteins characterised by a fibrillar morphology of typically 7–13 nm in diameter, a β-sheet secondary structure (known as cross-β) and ability to be stained by particular dyes, such as Congo red. In the human ...

after a chemical treatment with KOH and

IKI IKI may refer to: * Internationales Kulturinstitut, in Vienna * Iodine potassium-iodide, a chemical compound * Russian Space Research Institute, originally known as IKI RAN * Iki Airport, IATA code Iki or iki may refer to: * Iki Island, a Japane ...

. The

are globose to subglobose, simple, pale brown in color, with thick walls, and are passively released thanks to the mazaedium. The stalk has a

cortex Cortex or cortical may refer to: Biology * Cortex (anatomy), the outermost layer of an organ ** Cerebral cortex, the outer layer of the vertebrate cerebrum, part of which is the ''forebrain'' *** Motor cortex, the regions of the cerebral cortex i ...

, with the outermost layer composed of 1 or 2 rows of cylindrical aseptate

hyphae A hypha (; ) is a long, branching, filamentous structure of a fungus, oomycete, or actinobacterium. In most fungi, hyphae are the main mode of vegetative growth, and are collectively called a mycelium. Structure A hypha consists of one o ...

, dark reddish brown in color and tightly adhered. The stalk medulla (below the cortex) is composed of cylindrical

hyaline A hyaline substance is one with a glassy appearance. The word is derived from , and . Histopathology Hyaline cartilage is named after its glassy appearance on fresh gross pathology. On light microscopy of H&E stained slides, the extracellula ...

hyphae. The pigment granules on the external surface of ascomata appear

dark violet Violet is the color of light at the short wavelength end of the visible spectrum. It is one of the seven colors that Isaac Newton labeled when dividing the spectrum of visible light in 1672. Violet light has a wavelength between approximately ...

in water, but after partly dissolving in KOH they turn turquoise green. In particular, the species ''C. blascoi'' is defined as a

lichen A lichen ( , ) is a hybrid colony (biology), colony of algae or cyanobacteria living symbiotically among hypha, filaments of multiple fungus species, along with yeasts and bacteria embedded in the cortex or "skin", in a mutualism (biology), m ...

measuring 0.15–0.36 mm in height without mazaedium, with a

peridium The peridium is the protective layer that encloses a mass of spores in fungi. This outer covering is a distinctive feature of gasteroid fungi. Description Depending on the species, the peridium may vary from being paper-thin to thick and rubb ...

spherical or urniform in shape measuring 0.05–0.10 × 0.10–0.15 mm that appears dark violet when wet but black and shiny when dehydrated, with an ocre disk around the peridium, and a stalk measuring 0.10–0.26 × 0.02–0.04 mm that is smooth, black and shiny, sometimes with a broader base.

Ecology and distribution

''C. blascoi'' is a

saprobic Saprotrophic nutrition or lysotrophic nutrition is a process of chemoheterotrophic extracellular digestion involved in the processing of decayed (dead or waste) organic matter. It occurs in saprotrophs, and is most often associated with fungi ...

, either solitary or gregarious, found on the inner side of loose but still attached

bark Bark may refer to: Common meanings * Bark (botany), an outer layer of a woody plant such as a tree or stick * Bark (sound), a vocalization of some animals (which is commonly the dog) Arts and entertainment * ''Bark'' (Jefferson Airplane album), ...

strips. It has been observed in several

Cupressaceae Cupressaceae or the cypress family is a family of conifers. The family includes 27–30 genera (17 monotypic), which include the junipers and redwoods, with about 130–140 species in total. They are monoecious, subdioecious or (rarely) dioecio ...

species of trees, such as ''

Juniperus oxycedrus ''Juniperus oxycedrus'', vernacularly called Cade, cade juniper, prickly juniper, prickly cedar, or sharp cedar, is a species of juniper, native across the Mediterranean region, growing on a variety of rocky sites from sea level. The specific ep ...

'', '' J. thurifera'' and ''

Cupressus sempervirens ''Cupressus sempervirens'', the Mediterranean cypress (also known as Italian cypress, Tuscan cypress, Persian cypress, or pencil pine), is a species of cypress native to the eastern Mediterranean region and Iran. While some studies show it ha ...

''.
It is found in multiple Spanish provinces:

Ávila Ávila ( , , ) is a Spanish city located in the autonomous community of Castile and León. It is the capital and most populated municipality of the Province of Ávila. It lies on the right bank of the Adaja river. Located more than 1,130 m a ...

Burgos Burgos () is a city in Spain located in the autonomous community of Castile and León. It is the capital and most populous municipality of the province of Burgos. Burgos is situated in the north of the Iberian Peninsula, on the confluence of th ...

Madrid Madrid ( ; ) is the capital and List of largest cities in Spain, most populous municipality of Spain. It has almost 3.5 million inhabitants and a Madrid metropolitan area, metropolitan area population of approximately 7 million. It i ...

Soria Soria () is a municipality and a Spanish city, located on the Douro river in the east of the autonomous community of Castile and León and capital of the province of Soria. Its population is 38,881 ( INE, 2017), 43.7% of the provincial populatio ...

Toledo Toledo most commonly refers to: * Toledo, Spain, a city in Spain * Province of Toledo, Spain * Toledo, Ohio, a city in the United States Toledo may also refer to: Places Belize * Toledo District * Toledo Settlement Bolivia * Toledo, Or ...

and

Zaragoza Zaragoza (), traditionally known in English as Saragossa ( ), is the capital city of the province of Zaragoza and of the autonomous communities of Spain, autonomous community of Aragon, Spain. It lies by the Ebro river and its tributaries, the ...

Taxonomy

''Cryptocalicium blascoi'', along with its genus, family, order and subclass, were described in 2021 by Maria Prieto, Javier Etayo and Ibai Olariaga. The

holotype A holotype (Latin: ''holotypus'') is a single physical example (or illustration) of an organism used when the species (or lower-ranked taxon) was formally described. It is either the single such physical example (or illustration) or one of s ...

was found in La Cabilda Park, in

Hoyo de Manzanares Hoyo de Manzanares is a municipality of the Community of Madrid, Spain, and is located on the northwestern side of the Community of Madrid and to the south of the Sierra de Guadarrama The Sierra de Guadarrama (Guadarrama Mountains) is a moun ...

(

Spain Spain, or the Kingdom of Spain, is a country in Southern Europe, Southern and Western Europe with territories in North Africa. Featuring the Punta de Tarifa, southernmost point of continental Europe, it is the largest country in Southern Eur ...

). The

generic Generic or generics may refer to: In business * Generic term, a common name used for a range or class of similar things not protected by trademark * Generic brand, a brand for a product that does not have an associated brand or trademark, other ...

name "''Cryptocalicium''" refers to the calicioid (=mazaediate)

that occur hidden under the

, while the

specific epithet In Taxonomy (biology), taxonomy, binomial nomenclature ("two-term naming system"), also called binary nomenclature, is a formal system of naming species of living things by giving each a name composed of two parts, both of which use Latin gramm ...

"''blascoi''" is a reference to Javier Blasco Zumeta, an important

Aragon Aragon ( , ; Spanish and ; ) is an autonomous communities of Spain, autonomous community in Spain, coextensive with the medieval Kingdom of Aragon. In northeastern Spain, the Aragonese autonomous community comprises three provinces of Spain, ...

ese naturalist who showed the authors the first locality where this species was found and who provided additional material of it.

Phylogeny

Cryptocaliciomycetidae currently represents the closest

clade In biology, a clade (), also known as a Monophyly, monophyletic group or natural group, is a group of organisms that is composed of a common ancestor and all of its descendants. Clades are the fundamental unit of cladistics, a modern approach t ...

to the subclass

Eurotiomycetidae Eurotiomycetidae is a subclass of the Eurotiomycetes. Subdivisions According to Mycobank, Eurotiomycetidae is currently subdivided as follows: * Orders ** Arachnomycetales ** Ascosphaerales ** Coryneliales ** Elaphomycetales ** Eurotiales * ...

, as the cladogram shows.

References

{{Taxonbar, from1=Q118873929, from2=Q114152856 Taxa named by Javier Angel Etayo Salazar Taxa described in 2021 Eurotiomycetes Monotypic Eurotiomycetes genera