Computed Corpuscle Sectioning is a general method for determining the volume, profile area, and perimeter of a slab section of any computer-modeled three-dimensional object in any orientation and at any position. It was originally developed as a model of

cell nuclei The cell nucleus (; : nuclei) is a membrane-bound organelle found in eukaryotic cells. Eukaryotic cells usually have a single nucleus, but a few cell types, such as mammalian red blood cells, have no nuclei, and a few others including osteoc ...

in a tissue section in conjunction with the Reference Curve Method for correcting

ploidy Ploidy () is the number of complete sets of chromosomes in a cell, and hence the number of possible alleles for autosomal and pseudoautosomal genes. Here ''sets of chromosomes'' refers to the number of maternal and paternal chromosome copies, ...

measurements by

image analysis Image analysis or imagery analysis is the extraction of meaningful information from images; mainly from digital images by means of digital image processing techniques. Image analysis tasks can be as simple as reading barcode, bar coded tags or a ...

in a tissue section, but it is useful for evaluating any

algorithm In mathematics and computer science, an algorithm () is a finite sequence of Rigour#Mathematics, mathematically rigorous instructions, typically used to solve a class of specific Computational problem, problems or to perform a computation. Algo ...

that corrects ploidy measurements for the effect of sectioning. Computed Corpuscle Sectioning has obvious pertinence to

stereology Stereology is the three-dimensional interpretation of two-dimensional cross sections of materials or tissues. It provides practical techniques for extracting quantitative information about a three-dimensional material from measurements made on two ...

, but has not been exploited in that field. The patents on this method (U.S.

Patent A patent is a type of intellectual property that gives its owner the legal right to exclude others from making, using, or selling an invention for a limited period of time in exchange for publishing an sufficiency of disclosure, enabling discl ...

numbers 5,918,038, 6,035,258, and 6,603,869) are no longer in force.

References

*Freed JA. Possibility of correcting image cytometric DNA (ploidy) measurements in tissue sections: Insights from computed corpuscle sectioning and the reference curve method. Analyt Quant Cytol Histol 19(5):376-386, 1997

*Freed JA. Improved correction of quantitative nuclear DNA (ploidy) measurements in tissue sections. Analyt Quant Cytol Histol 21(2):103-112, 199

*Freed JA. Conceptual comparison of two computer models of corpuscle sectioning and of two algorithms for correction of ploidy measurements in tissue sections. Analyt Quant Cytol Histol 22(1): 17-25, 2000.

*"A general method for determining the volume and profile area of a sectioned corpuscle", U.S. Pat. No. 5,918,038 issued 6/29/99 to Jeffrey A. Freed

*"Method for correction of quantitative DNA measurements in a tissue section", U.S. Pat. No. 6,035,258 issued 3/7/00 to Jeffrey A. Freed

*“Use of perimeter measurements to improve ploidy measurements in a tissue section”, U.S. Pat. No. 6,603,869 issued 8/5/03 to Jeffrey A. Freed

3D computer graphics Medical imaging