Chesapeake and Ohio Railroad may refer to:
*
Chesapeake and Ohio Railway
The Chesapeake and Ohio Railway was a Class I railroad formed in 1869 in Virginia from several smaller Virginia railroads begun in the 19th century. Led by industrialist Collis Potter Huntington, it reached from Virginia's capital city of Rich ...
, an American railroad company between 1878–1987
*
Virginia Central Railroad, predecessor of the above, known as the Chesapeake and Ohio Railroad between 1868–1878
{{disambig