Ceruletide (

INN Inns are generally establishments or buildings where travelers can seek lodging, and usually, food and drink. Inns are typically located in the country or along a highway. Before the advent of motorized transportation, they also provided accomm ...

), also known as cerulein or caerulein, is a ten amino acid

oligopeptide An oligopeptide ('' oligo-'', "a few"), is a peptide consisting of two to twenty amino acids, including dipeptides, tripeptides, tetrapeptides, and other polypeptides. Some of the major classes of naturally occurring oligopeptides include aerugi ...

that stimulates

smooth muscle Smooth muscle is one of the three major types of vertebrate muscle tissue, the others being skeletal and cardiac muscle. It can also be found in invertebrates and is controlled by the autonomic nervous system. It is non- striated, so-called bec ...

and increases digestive secretions. Ceruletide is similar in action and composition to

cholecystokinin Cholecystokinin (CCK or CCK-PZ; from Greek ''chole'', "bile"; ''cysto'', "sac"; ''kinin'', "move"; hence, ''move the bile-sac (gallbladder)'') is a peptide hormone of the gastrointestinal system responsible for stimulating the digestion of fat a ...

. It stimulates gastric, biliary, and pancreatic secretion; and certain smooth muscle. It is used in paralytic ileus and as diagnostic aid in pancreatic malfunction. It is used to induce

pancreatitis Pancreatitis is a condition characterized by inflammation of the pancreas. The pancreas is a large organ behind the stomach that produces digestive enzymes and a number of hormone A hormone (from the Ancient Greek, Greek participle , "se ...

in experimental animal models. Ceruletide was discovered and its structure elucidated in 1967 by Australian and Italian scientists from dried skins of the

Australian green tree frog The Australian green tree frog (''Ranoidea caerulea''/''Litoria caerulea''), also known as simply green tree frog in Australia, White's tree frog, or dumpy tree frog, is a species of tree frog native to Australia and New Guinea, with Introduc ...

(''Ranoidea caerulea'', formerly ''Hyla caerulea''). Its amino acid sequence is Pglu-Gln-Asp-Tyr O₃HThr-Gly-Trp-Met-Asp-Phe-NH₂.

Induction of pancreatitis

Ceruletide upregulates pancreatic acinar cell

intercellular adhesion molecule-1 Cell adhesion molecules (CAMs) are a subset of cell surface proteins that are involved in the binding of cells with other cells or with the extracellular matrix (ECM), in a process called cell adhesion. In essence, CAMs help cells stick to each ...

(ICAM-1) proteins through intracellular upregulation of

NF-κB Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is a family of transcription factor protein complexes that controls transcription (genetics), transcription of DNA, cytokine production and cell survival. NF-κB is found i ...

. Surface ICAM-1 in turn promotes neutrophil adhesion onto acinar cells enhancing pancreatic

inflammation Inflammation (from ) is part of the biological response of body tissues to harmful stimuli, such as pathogens, damaged cells, or irritants. The five cardinal signs are heat, pain, redness, swelling, and loss of function (Latin ''calor'', '' ...

. In addition to promoting the inflammatory cell reaction to acinar cells, ceruletide induces pancreatitis through dysregulation of digestive enzyme production and cytoplasmic vacuolization, leading to acinar cell death and pancreatic edema. Ceruletide also activates

NADPH oxidase NADPH oxidase (nicotinamide adenine dinucleotide phosphate oxidase) is a membrane-bound enzyme complex that faces the extracellular space. It can be found in the plasma membrane as well as in the membranes of phagosomes used by neutrophil white ...

, a source of reactive oxygen species contributing to inflammation, as well as the

Janus kinase Janus kinase (JAK) is a family of intracellular, non-receptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway. They were initially named "just another kinase" 1 and 2 (since they were just two of many discoverie ...

/signal transducer, another inflammation inducer.

References

{{Diagnostic agents Peptides

Induction of pancreatitis

See also

References