CHES (''N''-cyclohexyl-2-aminoethanesulfonic acid) is a
buffering agent
A buffer solution is a solution where the pH does not change significantly on dilution or if an acid or base is added at constant temperature. Its pH changes very little when a small amount of strong acid or base is added to it. Buffer solution ...
. CHES buffers have a useful range of pH 8.6–10.
It typically appears as a white crystalline powder.
Effect of impurities
Commercial prep of CHES (and other
sulfonylethyl buffers like
MES,
BES, and
PIPES
Pipe(s), PIPE(S) or piping may refer to:
Objects
* Pipe (fluid conveyance), a hollow cylinder following certain dimension rules
** Piping, the use of pipes in industry
* Smoking pipe
** Tobacco pipe
* Half-pipe and quarter pipe, semi-circu ...
) can contain a contaminant oligo(vinylsulfonic acid) (OVS), which is a polyanionic mimic of RNA, and can be a potent (
pM) inhibitor of RNA binding proteins and enzymes.
References
External links
*
Buffer solutions
Sulfonic acids
{{OrganicAcid-stub