The Buehler test is an ''
in vivo
Studies that are ''in vivo'' (Latin for "within the living"; often not italicized in English) are those in which the effects of various biological entities are tested on whole, living organisms or cells, usually animals, including humans, an ...
'' test to screen for substances that cause human skin sensitisation (i.e.
allergens
An allergen is an otherwise harmless substance that triggers an allergic reaction in sensitive individuals by stimulating an immune response.
In technical terms, an allergen is an antigen that is capable of stimulating a type-I hypersensitivi ...
). It was first proposed by Edwin Vernon Buehler in 1965 and further explained in 1980.
It is a non-
adjuvant
In pharmacology, an adjuvant is a drug or other substance, or a combination of substances, that is used to increase the efficacy or potency of certain drugs. Specifically, the term can refer to:
* Adjuvant therapy in cancer management
* Anal ...
test. In the test, guinea pigs are exposed to a high dose of the substance. They are then given a challenge dose, which is the highest dose that does not cause irritation.
[{{cite web , author=OECD , authorlink=Organisation for Economic Co-operation and Development , title=Test No. 406: Skin Sensitisation , work=]OECD Guidelines for the Testing of Chemicals
OECD Guidelines for the Testing of Chemicals (OECD TG) are a set of internationally accepted specifications for the testing of chemicals decided on by the Organisation for Economic Co-operation and Development (OECD). They were first published in ...
, Section 4 , doi=10.1787/9789264070660-en , publisher=OECD Publishing , date=1992-07-17
, url=http://www.oecd-ilibrary.org/environment/test-no-406-skin-sensitisation_9789264070660-en , accessdate=2012-02-17
References
Allergology