BOSC 23 is a human
kidney
In humans, the kidneys are two reddish-brown bean-shaped blood-filtering organ (anatomy), organs that are a multilobar, multipapillary form of mammalian kidneys, usually without signs of external lobulation. They are located on the left and rig ...
cell line
An immortalised cell line is a population of cells from a multicellular organism that would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells ...
developed by Warren Pear in David Baltimore's lab that was derived from the
293T cell line. The main use of BOSC 23 is the production of recombinant
retroviruses
A retrovirus is a type of virus that inserts a DNA copy of its RNA genome into the DNA of a host cell that it invades, thus changing the genome of that cell. After invading a host cell's cytoplasm, the virus uses its own reverse transcriptase ...
; it stably expresses Moloney
murine leukemia virus
The murine leukemia viruses (MLVs or MuLVs) are retroviruses named for their ability to cause cancer in murine (mouse) hosts. Some MLVs may infect other vertebrates. MLVs include both exogenous and endogenous viruses. Replicating MLVs have a p ...
proteins and when
transiently transfected with recombinant retroviral vector DNA, produces high titers of infectious retroviral particles. The cell line does not produce detectable replication-competent virus, an important safety feature.
BOSC 23 carries
neomycin
Neomycin, also known as framycetin, is an aminoglycoside antibiotic that displays bactericidal activity against Gram-negative aerobic bacilli and some anaerobic bacilli where resistance has not yet arisen. It is generally not effective against ...
/
G418
G418 (geneticin) is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is produced by '' Micromonospora rhodorangea''. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cell ...
resistance derived from its parental line 293T, and also
hygromycin Hygromycin may refer to either of two chemically dissimilar antibiotics produced by the bacterium ''Streptomyces hygroscopicus'':
* Hygromycin A
* Hygromycin B, an aminoglycoside that kills bacteria, fungi and higher eukaryotic
The eukaryot ...
and
mycophenolic acid
Mycophenolic acid is an immunosuppressant medication used to prevent rejection following organ transplantation and to treat autoimmune conditions such as Crohn's disease and lupus. Specifically it is used following kidney, heart, and live ...
(gpt) resistance. It should be maintained under gpt selection.
This cell line is a model for
cancer
Cancer is a group of diseases involving Cell growth#Disorders, abnormal cell growth with the potential to Invasion (cancer), invade or Metastasis, spread to other parts of the body. These contrast with benign tumors, which do not spread. Po ...
research, with emphasis on the lack of activated
Src protein.
[Goh YM, Cinghu S, Hong ETH et al. Src kinase phosphorylates RUNX3 at tyrosine residues and localizes the protein in the cytoplasm. The Journal of Biochemistry vol.285, no.13, pp.10122-10129, March 2010]
References
External links
Cellosaurus entry for BOSC23
{{DEFAULTSORT:Bosc23
Kidney cancer
Human cell lines