BAF agar or biotin-aneurin-folic acid agar is a type of

agar Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from " ogonori" and " tengusa". As found in nature, agar is a mixture of two components, t ...

growth medium containing

peptones Peptides are short chains of amino acids linked by peptide bonds. A polypeptide is a longer, continuous, unbranched peptide chain. Polypeptides that have a molecular mass of 10,000 Da or more are called proteins. Chains of fewer than twenty am ...

. It is used to grow cultures of

mycorrhiza A mycorrhiza (; , mycorrhiza, or mycorrhizas) is a symbiotic association between a fungus and a plant. The term mycorrhiza refers to the role of the fungus in the plant's rhizosphere, the plant root system and its surroundings. Mycorrhizae play ...

fungi A fungus (: fungi , , , or ; or funguses) is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and mold (fungus), molds, as well as the more familiar mushrooms. These organisms are classified as one ...

. It was first described by in ''Nutritional requirements of

Lactarius ''Lactarius'' is a genus of mushroom-producing, ectomycorrhizal fungi, containing several Edible mushroom, edible species. The species of the genus, common name, commonly known as milk-caps, are characterized by the milky fluid ("latex") they ex ...

species and cultural characters in relation to taxonomy'' in 1981. The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.

Typical composition

BAF agar typically contains: * 30.0 g/L

glucose Glucose is a sugar with the Chemical formula#Molecular formula, molecular formula , which is often abbreviated as Glc. It is overall the most abundant monosaccharide, a subcategory of carbohydrates. It is mainly made by plants and most algae d ...

* 2.0 g/L

peptone Peptides are short chains of amino acids linked by peptide bonds. A polypeptide is a longer, continuous, unbranched peptide chain. Polypeptides that have a molecular mass of 10,000 Da or more are called proteins. Chains of fewer than twenty ami ...

* 0.2 g/L

yeast extract Yeast extracts consist of the cell contents of yeast without the cell walls; they are used as food additives or flavorings, or as nutrients for bacterial culture Growing media, media. They are often used to create savoury flavors and umami tast ...

* 0.5 g/L KH₂PO₄ * 0.5 g/L MgSO₄.7 H₂O * 10.0 mg/L FeCl₃.6 H₂O * 1.0 mg/L ZnSO₄.7 H₂O * 5.0 mg/L MnSO₄ * 100.0 mg/L CaCl₂.2 H₂O * 50.0 μg/L thiamine HCl * 1.0 μg/L

biotin Biotin (also known as vitamin B7 or vitamin H) is one of the B vitamins. It is involved in a wide range of metabolic processes, both in humans and in other organisms, primarily related to the utilization of fats, carbohydrates, and amino acids. ...

* 100.0 μg/L

folic acid Folate, also known as vitamin B9 and folacin, is one of the B vitamins. Manufactured folic acid, which is converted into folate by the body, is used as a dietary supplement and in food fortification as it is more stable during processing and ...

* 50.0 μg/L

inositol In biochemistry, medicine, and related sciences, inositol generally refers to ''myo''-inositol (formerly ''meso''-inositol), the most important stereoisomer of the chemical compound cyclohexane-1,2,3,4,5,6-hexol. Its elemental formula, formula is ...

* 15 g/L

References

Microbiological media {{Microbiology-stub