The B3
DNA binding domain (DBD) is a
highly conserved
In evolutionary biology, conserved sequences are identical or similar sequences in nucleic acids ( DNA and RNA) or proteins across species ( orthologous sequences), or within a genome ( paralogous sequences), or between donor and receptor taxa ( ...
domain found exclusively in
transcription factors (≥40 species) () combined with other domains (). It consists of 100-120 residues, includes seven
beta strands and two
alpha helices that form a DNA-binding pseudobarrel
protein fold (); it interacts with the major groove of
DNA.
B3 families
In ''
Arabidopsis thaliana
''Arabidopsis thaliana'', the thale cress, mouse-ear cress or arabidopsis, is a small flowering plant native to Eurasia and Africa. ''A. thaliana'' is considered a weed; it is found along the shoulders of roads and in disturbed land.
A winter a ...
'', there are three main families of transcription factors that contain B3 domain:
* ARF (
Auxin Response Factors)
* ABI3 (
ABscisic acid Insensitive3)
* RAV (Related to ABI3/VP1)
and
are only known
NMR solution phase structures of the B3 DNA Binding Domain.
Related proteins
The
N-terminal
The N-terminus (also known as the amino-terminus, NH2-terminus, N-terminal end or amine-terminus) is the start of a protein or polypeptide, referring to the free amine group (-NH2) located at the end of a polypeptide. Within a peptide, the ami ...
domain of
restriction endonuclease EcoRII; the
C-terminal domain of
restriction endonuclease BfiI possess a similar DNA-binding pseudobarrel
protein fold.
See also
*Restriction endonuclease
EcoRII
*
Auxin
Auxins (plural of auxin ) are a class of plant hormones (or plant-growth regulators) with some morphogen-like characteristics. Auxins play a cardinal role in coordination of many growth and behavioral processes in plant life cycles and are essenti ...
*
Abscisic acid
Abscisic acid (ABA) is a plant hormone. ABA functions in many plant developmental processes, including seed and bud dormancy, the control of organ size and stomatal closure. It is especially important for plants in the response to environmental s ...
References
External links
DBD database of predicted transcription factors Uses a curated set of DNA-binding domains to predict transcription factors in all completely sequenced genomes
*Classification in the "Transcription factors" table according to th
database.
Database of Arabidopsis Transcription FactorsB3
RAV
an
ARF
family a
PlantTFDB:Plant Transcription Factor Database
{{Transcription factors, g0
Molecular genetics
Gene expression
Transcription factors
Protein domains