An aster is a cellular structure shaped like a

star A star is a luminous spheroid of plasma (physics), plasma held together by Self-gravitation, self-gravity. The List of nearest stars and brown dwarfs, nearest star to Earth is the Sun. Many other stars are visible to the naked eye at night sk ...

, consisting of a

centrosome In cell biology, the centrosome (Latin centrum 'center' + Greek sōma 'body') (archaically cytocentre) is an organelle that serves as the main microtubule organizing center (MTOC) of the animal cell, as well as a regulator of cell-cycle progre ...

and its associated microtubules during the early stages of

mitosis Mitosis () is a part of the cell cycle in eukaryote, eukaryotic cells in which replicated chromosomes are separated into two new Cell nucleus, nuclei. Cell division by mitosis is an equational division which gives rise to genetically identic ...

in an

animal Animals are multicellular, eukaryotic organisms in the Biology, biological Kingdom (biology), kingdom Animalia (). With few exceptions, animals heterotroph, consume organic material, Cellular respiration#Aerobic respiration, breathe oxygen, ...

cell. Asters do not form during mitosis in

plants Plants are the eukaryotes that form the kingdom Plantae; they are predominantly photosynthetic. This means that they obtain their energy from sunlight, using chloroplasts derived from endosymbiosis with cyanobacteria to produce sugars f ...

. Astral rays, composed of microtubules, radiate from the centrosphere and look like a cloud. Astral rays are one variant of microtubule which comes out of the centrosome; others include kinetochore microtubules and polar microtubules. During mitosis, there are five stages of cell division: Prophase, Prometaphase, Metaphase, Anaphase, and Telophase. During prophase, two aster-covered centrosomes migrate to opposite sides of the nucleus in preparation of mitotic spindle formation. During prometaphase there is fragmentation of the nuclear envelope and formation of the mitotic spindles. During metaphase, the kinetochore microtubules extending from each centrosome connect to the centromeres of the chromosomes. Next, during anaphase, the kinetochore microtubules pull the sister chromatids apart into individual chromosomes and pull them towards the centrosomes, located at opposite ends of the cell. This allows the cell to divide properly with each daughter cell containing full replicas of chromosomes. In some cells, the orientation of the asters determines the plane of division upon which the cell will divide.

Astral microtubules

Astral microtubules are a subpopulation of

microtubule Microtubules are polymers of tubulin that form part of the cytoskeleton and provide structure and shape to eukaryotic cells. Microtubules can be as long as 50 micrometres, as wide as 23 to 27 nanometer, nm and have an inner diameter bet ...

s, which only exist during and immediately before

. They are defined as any microtubule originating from the

which does not connect to a kinetochore.Mitosis
Molecular Biology of the Cell, Albert et al 4th Edition. Astral microtubules develop in the actin skeleton and interact with the cell cortex to aid in spindle orientation. They are organized into radial arrays around the centrosomes. The turn-over rate of this population of microtubules is higher than any other population. The role of astral microtubules is assisted by dyneins specific to this role. These dyneins have their light chains (static portion) attached to the cell membrane, and their globular parts (dynamic portions) attached to the microtubules. The globular chains attempt to move towards the centrosome, but as they are bound to the cell membrane, this results in pulling the centrosomes towards the membrane, thus assisting cytokinesis. Astral microtubules are not required for the progression of mitosis, but they are required to ensure the fidelity of the process. The function of astral microtubules can be generally considered as determination of cell geometry. They are absolutely required for correct positioning and orientation of the mitotic spindle apparatus, and are thus involved in determining the cell division site based on the geometry and polarity of the cells. The maintenance of astral microtubules is dependent on centrosomal integrity. It is also dependent on several microtubule-associated proteins such as EB1 and adenomatous polyposis coli (APC). Growth of Microtubules Asters grow through nucleation and polymerization. At the negative ends of the aster centrosomes will nucleate (form a nucleus) and anchor to the microtubules. At the positive end, polymerization of the aster will occur. Cortical dynein, a motor protein, moves along the microtubules of the cell and plays a key role in the growth and inhibition of aster microtubules. A dynein that is barrier-attached can inhibit and trigger growth.

References

* Ishihara, Keisuke, et al. "Physical basis of large microtubule aster growth." ''eLife'', vol. 5, 2016. ''Gale OneFile: Health and Medicine'', link.gale.com/apps/doc/A476395269/HRCA?u=cuny_hunter&sid=HRCA&xid=5e6ad228. Accessed 28 Apr. 2021. * Laan, Liedewij et al. “Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces That Position Microtubule Asters.” ''Cell (Cambridge)'' 148.3 (2012): 502–514. Web.

Astral microtubules

References

See also