Argentation chromatography is

chromatography In chemical analysis, chromatography is a laboratory technique for the Separation process, separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it ...

using a stationary phase that contains silver salts. Silver-containing stationary phases are well suited for separating organic compounds on the basis of the number and type of alkene groups. The technique is employed for

gas chromatography Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for Separation process, separating and analyzing compounds that can be vaporized without Chemical decomposition, decomposition. Typical uses of GC include t ...

and various types of liquid chromatography, including

thin layer chromatography Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. This is called the sta ...

. Analytes containing alkene groups elute more slowly than the analogous compounds lacking alkenes. Separations are also sensitive to the type of alkene. The technique is especially useful in the analysis of fats and fatty acids, which are well known to exist in both saturated and unsaturated (alkene-containing) forms. For example,

trans fats Trans fat is a type of unsaturated fat that occurs in foods. Small amounts of trans fats occur naturally, but large amounts are found in some processed foods made with partially hydrogenated oils. Because consumption of trans fats is associated ...

, undesirable contaminants in

ultra-processed food An ultra-processed food (UPF) is a grouping of processed food characterized by relatively involved methods of production. There is no simple definition of UPF, but they are generally understood to be an industrial creation derived from natural ...

s, are quantified by argentation chromatography.

Theory

Silver ions form alkene complexes. The binding is reversible, but sufficient to impede the elution of the alkene-containing analytes.

References

{{chromatography Chromatography