Apis andreniformis

''Apis andreniformis'', or the black dwarf honey bee, is a relatively rare species of honey bee whose native habitat is the tropical and subtropical regions of Southeast Asia.

''A. andreniformis'' was the fifth honey bee species to be described of the seven known species of '' Apis''. Until recently, however, the actual identity of the species was poorly understood. It was not recognized as its own species, but was instead considered to be a part of the species ''Apis florea.'' Recent studies have highlighted notable differences between the bees and have thus separated them into distinct species.

Taxonomy and phylogeny

''Apis andreniformis'' is a part of the family Apidae, which includes honey, cuckoo, carpenter, digger, bumble, and stingless bees. The genus ''Apis'' includes honey bees, the most common being ''Apis mellifera'', otherwise known as the Western honey bee. ''A. andreniformis'' is most closely related to ''Apis florea'', its sister species with which it is commonly seen in sympatric distribution throughout southeast Asia.

Description and identification

Physical characteristics

''A. andreniformis'' can be distinguished from other ''Apis'' species by noting their dark black coloration, making them the darkest of their genus. Originally, it was thought that ''A. andreniformis'' was a part of the species ''A. florea'', but recent studies have noted morphological differences that have separated the two. Some distinctions include: structural differences in the endophalli, a larger wing venation in ''A. andreniformis'', and a longer basitarsal extension in ''A. florea''. Additionally, there are slight color variations between the two species. In typical ''A. andreniformis'', its first two abdominal segments are black and its scutellum is reddish brown, while in ''A. florea'', the first two abdominal segments are reddish brown and their scutellum is black, though there is some variation that makes color unreliable. Another distinguishing factor is the presence of black hairs on the tibia of ''A. andreniformis'', which are white in ''A. florea''.

Other differentiating characteristics include cubital indexes and proboscis length. ''A. andreniformis'' has an index of 6.37, while ''A. florea'' has one of 2.86. The proboscis of ''A. andreniformis'' has a length of 2.80 mm, while that of ''A. florea'' is 3.27 mm.

Within the species, queens can typically be distinguished from workers and drones by their near entire black coloration.

Nest structure

''A. andreniformis'' nests are made of a single comb found hanging from small twigs in quiet forests, generally in darker areas where there is 25 to 30% of normal sunlight. This type of nest is called an open-air nest. They are commonly found hanging in small trees, shrubs, or bushes are usually hidden behind leaves or branches to avoid detection. They are usually built between 1 and 15 meters from the ground, though the average altitude is 2.5 m. The honeycomb typically ranges from 70 to 90 mm in size. This nest is distinct from other Apis species, like '' A. mellifera'', who build their nests inside of cavities. This open-air structure—found also in ''A. florea'', '' A. dorsata'', and '' A. laboriosa''—along with a relatively flat line of nectar cells along the top, creates a plateau above the nest that can be used as a stage for their communication method known as the waggle dance.

While creating the nest, plant resin is placed along the supporting branch and around the edges of the nest. This acts as a barrier against small insects, like ants, that may try to enter the nest. The major location of honey storage can be found in the area above and surrounding the branch. The entire area below the honey storage and branch is the brood area, where larva development occurs. Along the top of the brood area is the location of pollen storage. Drone development occurs in the cells toward the bottom of the nest, while queen cells can be found protruding vertically.

Distribution and habitat

''A. andreniformis'' is found in southeast Asia, specifically southern China, India, Burma, Laos, Vietnam, Thailand, Malaysia, Indonesia, and the Philippines. They are commonly found in sympatric distribution with ''A. florea''. Although they are sympatrically distributed, it is uncommon to have nests of different species in the same tree or bush. Each species tends to be found closer to nests of its own species rather than its sister species. ''A. andreniformis'' is considered a lowland species because they are most commonly found in elevations below 1,000 m, although they may migrate to higher elevations during rainy seasons. Similarly, they are found in tropical and subtropical regions, while cavity-dwelling honey bees can be found in colder climates.

Behavior

Queen determination

Queens are not genetically determined so any young female larva can become either a worker or a queen. In order to create a queen cell, royal jelly must be fed to the female larva. In cases of an unexpected loss of a queen, royal jelly is fed to a female worker larva to create a new emergency queen. Although emergency queen rearing is possible, most often, the loss of a queen results in the dispersal of a colony. If a queen becomes separated from her hive, the workers will leave the nest to search for her. Finding and joining a queen is an innate response for workers. The workers' strong attraction to queens is seen when a queen is lost, after a hive disruption by a predator, and during colony migration.

Queenless and interspecific colonies

In an ''Apis andrenformis'' queenright colony, worker policing occurs, which prevents workers from laying their own eggs and helps maintain the dominance hierarchy. After the loss of a queen, workers will first try to rear a new queen using royal jelly. If this fails, worker policing will decrease and workers will activate their ovaries in order to rear more drones before the colony dies. It is also possible for queenless colonies to adopt a queen from a related species, specifically ''Apis florea''. This may suggest that worker bees of ''A. andreniformis ''are attracted to queen bee pheromones, even those belonging to other species in the same genera. Once inside the colony of ''A. florea'', the non-natal workers of ''A. andreniformis'' may attempt to lay their own eggs in order to continue propagating their species, an action known as reproductive parasitism. In a study conducted by Sitthipong Wongvilas et al., they found that most eggs laid by the non-natal ''A. andreniformis'' were policed by the queenright workers of the ''A. florea'' colony. Although they adopted ''A. andreniformis'' workers in the hive, ''A. florea'' workers policed non-natal larvae, thereby preventing interspecies parasitism. Queenright colonies of ''A. andreniformis'' have also been observed to adopt queenless workers of ''A. florea'' as well, but their policing on non-natal larvae has not yet been studied.

Dominance hierarchy

Unlike cavity-dwelling honey bees whose queen has a distinct chemical signal from that of the worker bees, ''A. andreniformis'' queens have similar chemical signals as their workers. Chemical signals secreted from the mandibular gland in ''A. andreniformis'' are not caste-determining like it is in other honey bees. As stated previously, the presence of royal jelly on young female larva produced the queen bee. Drones, or male bees, are not used for pollination or honey production, but are instead used only to mate with the queen.

Communication

Different types of honey bees may use different types of dances to communicate with their hive. Most cavity-dwelling species use vertical waggle dances, while open-air nesters do not perform a gravity oriented waggle dance and instead perform a horizontal dance. The shape of the nest creates a platform above the nest that can be used as a stage for communication. The dance is a straight run pointing directly to the source of pollen or nectar that the forager has visited. Since the dance of other Apis species is vertical, it is not actually directed towards the food source, as it is in ''A. andreniformis''.

Mating behavior

Queens of ''A. andreniformis'' commonly engage in polyandry, where the queen will mate with multiple drones, usually about 10-20 times in total. Due to the large amount of mating, queens must expel any excess semen. Excessive mating puts females at increased risk to predation since it must occur during flight and outside the nest in the open air. She is also at risk for sexually transmitted diseases and injury from unexpected inclement weather. Some Apis males put a "mating sign" in the sting chamber of the queen that she is unable to remove. This prevents her from avoiding unwanted copulation with other drones. In contrast, this sign is not found in ''A. andreniformis'', suggesting that queens have control over the number of mates they copulate with. Although there is a lot of risk to the queen, benefits may arise from the increased genetic diversity within the colony. Genetic diversity can lead to increased resistance to disease and illnesses.

Kin selection

In studies, ''A. andreniformis'' has shown a lack of recognition for its own species and nestmates. This has been shown in studies where queenless colonies of ''A. florea'' have joined the colony of ''A. andreniformis'' without facing aggression upon their initial arrival. Similarly, queenless colonies of ''A. andreniformis'' have been seen to join ''A. florea'' colonies, but in these cases, any ''A. andreniformis'' larvae are usually destroyed by the host colony, preventing parasitism from the foreign species. Worker bees of ''A. florea'' have complete reproductive dominance over ''A. Andreniformis'' in a queenless nest because they have recognition and kin selection, while ''A. Andreniformis'' does not. However, when a queen is present, worker bees do not reproduce and parasitism is turned off.

Parasites

The main parasites of both ''A. andreniformis'' and ''A. florea'' belong to genus '' Euvarroa''. However, ''A. andreniformis'' is attacked by the species '' Euvarroa wongsirii'', while '' Euvarroa sinhai'' preys on ''A. florea'', although ''Euvarroa sinhai'' have been found in hive debri of A. mellifera colonies it has not yet been confirmed to parasitize on them. The two species of ''Euvarroa'' have morphological and biological differences: while ''E. wongsirii'' has a triangular body shape and a length of 47 to 54 micrometres, ''E. sinhai'' has a more circular shape and a length of 39 to 40 micrometres.

Human importance

Honey bees, as a whole, tend to provide many useful products for human consumption. For ''A. andreniformis'' specifically, some commercial products include royal jelly, wax, honey, and bee venoms. Additionally, they are important for the pollination of flowers and plants.

References

{{Taxonbar, from=Q244070

andreniformis
Hymenoptera of Asia

Insects described in 1858
Taxa named by Frederick Smith (entomologist)