Four non-coding

small RNA Small RNA (sRNA) are polymeric RNA molecules that are less than 200 nucleotides in length, and are usually non-coding. RNA silencing is often a function of these molecules, with the most common and well-studied example being RNA interference (RNA ...

s containing a Fur box-like (

ferric uptake regulator In molecular biology, the ferric uptake regulator family is a family of bacterial proteins involved in regulating metal ion uptake and in metal homeostasis. The family is named for its founding member, known as the ferric uptake regulator or ferr ...

) sequence were identified by bioinformatics analysis in ''

Aggregatibacter actinomycetemcomitans ''Aggregatibacter actinomycetemcomitans'' is a Gram-negative, facultative anaerobe, nonmotile bacterium that is often found in association with localized aggressive periodontitis, a severe infection of the periodontium. It is also suspected to b ...

'' ''HK1651'' called JA01-JA04. The transcription of sRNAs was confirmed by Northern blot. Fur binding was demonstrated to each sRNA promoter, and that transcription of the sRNAs was decreased in presence of iron and increased by iron limitation. JA03 may have the ability to regulate biofilm formation. JA01 is conserved only among ''A. actinomycetemcomitans''. JA02 is present in both ''A. actinomycetemcomitans and P. multocida. J''A 03 and JA04 are most widely conserved and have orthologues across many ''

Pasteurellaceae The Pasteurellaceae comprise a large family of Gram-negative bacteria. Most members live as commensals on mucosal surfaces of birds and mammals, especially in the upper respiratory tract. Pasteurellaceae are typically rod-shaped, and are a notabl ...

''. HrrF RNA is another Fur-regulated sRNA conserved among the ''Pasteurcellaceae''.

References

Non-coding RNA {{molecular-cell-biology-stub

See also

References