A65 or A-65 may refer to:
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A65 road (England), a major road in England
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A65 motorway (France), a major road in France
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A65 motorway (Germany), a road connecting Kandel and Wörth am Rhein
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A65 motorway (Netherlands)
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A65 motorway (Spain)
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Benoni Defense, in the Encyclopaedia of Chess Openings
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BSA A65 Rocket, a motorcycle made by BSA
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