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White Spot Disease
''Ichthyophthirius multifiliis'', often termed "Ich", is a parasitic ciliate described by the French parasitologist Fouquet in 1876. Only one species is found in the genus which also gave name to the family. The name literally translates as "the fish louse with many children". The parasite can infect most freshwater fish species and, in contrast to many other parasites, shows low host specificity. It penetrates gill epithelia, skin and fins of the fish host and resides as a feeding stage (the trophont) inside the epidermis. It is visible as a white spot on the surface of the fish but, due to its internal microhabitat, it is a true endoparasite and not an ectoparasite. It causes a disease commonly referred to as white spot disease due to the macroscopically visible trophonts (up to 1 mm in diameter) in the skin and fins. The trophont, continuously rotating, is surrounded by host cells (epidermal cells and leukocytes), producing a minute elevation of the skin. These light-ref ...
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Cichlid
Cichlids () are a large, diverse, and widespread family of percomorph fish in the family Cichlidae, order Cichliformes. At least 1,760 species have been scientifically described, making it one of the largest vertebrate families, with only the Cyprinidae being more speciose. New species are discovered annually, and many species remain undescribed. The actual number of species is therefore unknown, with estimates varying between 2,000 and 3,000. They are native to the Neotropics, Africa (including Madagascar), the Middle East, and the Indian subcontinent, although some species have been introduced worldwide. Many cichlids, particularly tilapia, are important food fishes, while others, such as the '' Cichla'' species, are valued game fish. The family also includes many popular freshwater aquarium fish kept by hobbyists, including the angelfish, oscars, and discus. Cichlids have the largest number of endangered species among vertebrate families, most in the haplochrom ...
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Rainbow Trout
The rainbow trout (''Oncorhynchus mykiss'') is a species of trout native to cold-water tributary, tributaries of the Pacific Ocean in North America and Asia. The steelhead (sometimes called steelhead trout) is an Fish migration#Classification, anadromous (sea-run) form of the coastal rainbow trout or Columbia River redband trout that usually returns to freshwater to Spawn (biology), spawn after living two to three years in the ocean. Adult freshwater stream rainbow trout average between , while lake-dwelling and anadromous forms may reach . Coloration varies widely based on subspecies, forms, and habitat. Adult fish are distinguished by a broad reddish stripe along the lateral line, from gills to the tail, which is most vivid in breeding males. Wild-caught and Fish hatchery, hatchery-reared forms of the species have been transplanted and introduced for food or sport in at least 45 countries and every continent except Antarctica. Introductions to locations outside their nativ ...
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Copper Sulfate
Copper sulfate may refer to: * Copper(II) sulfate, CuSO4, a common, greenish blue compound used as a fungicide and herbicide * Copper(I) sulfate, Cu2SO4, an unstable white solid which is uncommonly used {{chemistry index Copper compounds ...
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Formalin
Formaldehyde ( , ) (systematic name methanal) is an organic compound with the chemical formula and structure , more precisely . The compound is a pungent, colourless gas that polymerises spontaneously into paraformaldehyde. It is stored as aqueous solutions (formalin), which consists mainly of the hydrate CH2(OH)2. It is the simplest of the aldehydes (). As a precursor to many other materials and chemical compounds, in 2006 the global production of formaldehyde was estimated at 12 million tons per year. It is mainly used in the production of industrial resins, e.g., for particle board and coatings. Formaldehyde also occurs naturally. It is derived from the degradation of serine, dimethylglycine, and lipids. Demethylases act by converting N-methyl groups to formaldehyde. Formaldehyde is classified as a group 1 carcinogen and can cause respiratory and skin irritation upon exposure. Forms Formaldehyde is more complicated than many simple carbon compounds in that it ...
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Malachite Green
Malachite green is an organic compound that is used as a dyestuff and controversially as an antimicrobial in aquaculture. Malachite green is traditionally used as a dye for materials such as silk, leather, and paper. Despite its name the dye is not prepared from the mineral malachite; the name just comes from the similarity of color. Structures and properties Malachite green is classified in the dyestuff industry as a triarylmethane dye and also using in pigment industry. Formally, malachite green refers to the chloride salt , although the term malachite green is used loosely and often just refers to the colored cation. The oxalate salt is also marketed. The anions have no effect on the color. The intense green color of the cation results from a strong absorption band at 621 nm ( extinction coefficient of ). Malachite green is prepared by the condensation of benzaldehyde and dimethylaniline to give leuco malachite green (LMG): : Second, this colorless leuco compound, ...
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Ichthyophthirius Multifiliis
''Ichthyophthirius multifiliis'', often termed "Ich", is a parasitic ciliate described by the French Parasitology, parasitologist Fouquet in 1876. Only one species is found in the genus which also gave name to the family. The name literally translates as "the fish louse with many children". The parasite can infect most freshwater fish species and, in contrast to many other parasites, shows low host specificity. It penetrates gill epithelia, skin and fins of the fish host and resides as a feeding stage (the trophont) inside the epidermis. It is visible as a white spot on the surface of the fish but, due to its internal microhabitat, it is a true endoparasite and not an Parasitism, ectoparasite. It causes a disease commonly referred to as white spot disease due to the macroscopically visible trophonts (up to 1 mm in diameter) in the skin and fins. The trophont, continuously rotating, is surrounded by host cells (epidermal cells and leukocytes), producing a minute elevation of ...
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Real-Time PCR
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively and semi-quantitatively (i.e., above/below a certain amount of DNA molecules). Two common methods for the detection of PCR products in real-time PCR are (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence. The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation ''qPCR'' be used for quantitative real-time PCR and that '' ...
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Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation. Mullis and biochemist Michael Smith (chemist), Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of Ancient DNA, ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications including biomedical research and forensic ...
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Light Microscope
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible spectrum, visible light and a system of lens (optics), lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve optical resolution, resolution and sample contrast (vision), contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope. In high-power microscopes, both eyepieces typically show the same image, but with a stereo microscope, slightly different images are used to create a 3-D effect. A camera is typically used to capture the image (micrograph). The sample can be lit in a variety of ways. Transparent objects can be lit from below and solid objects can be lit with light coming through ...
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Ichthyophthirius Multifiliis Theront
''Ichthyophthirius multifiliis'', often termed "Ich", is a parasitic ciliate described by the French parasitologist Fouquet in 1876. Only one species is found in the genus which also gave name to the family. The name literally translates as "the fish louse with many children". The parasite can infect most freshwater fish species and, in contrast to many other parasites, shows low host specificity. It penetrates gill epithelia, skin and fins of the fish host and resides as a feeding stage (the trophont) inside the epidermis. It is visible as a white spot on the surface of the fish but, due to its internal microhabitat, it is a true endoparasite and not an ectoparasite. It causes a disease commonly referred to as white spot disease due to the macroscopically visible trophonts (up to 1 mm in diameter) in the skin and fins. The trophont, continuously rotating, is surrounded by host cells (epidermal cells and leukocytes), producing a minute elevation of the skin. These light-refl ...
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Clown Loach
The clown loach (''Chromobotia macracanthus''), or tiger botia, is a tropical freshwater fish belonging to the botiid loach family. It is the monotypic, sole member of the genus ''Chromobotia''. It originates in inland waters in Indonesia on the islands of Sumatra and Borneo. The fish is called ''ulanguli'' by the locals in Sentarum, West Borneo. It is a popular fish in the freshwater aquarium trade and is sold worldwide. Taxonomy and naming The fish was first described as ''Cobitis macracanthus'' by Pieter Bleeker in 1852. In 1989, its scientific name was changed to ''Botia macracanthus''. In 2004, Maurice Kottelat, Dr. Maurice Kottelat divided the genus ''Botia'', containing 47 different species, into seven separate genus, genera, resulting in the clown loach being placed in a genus of its own, ''Chromobotia''. Its scientific name roughly translates to "Large-thorned colourful warrior". The common name "clown loach" comes from the fish's bright colours and stripes (often tropi ...
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Siamese Fighting Fish
The Siamese fighting fish (''Betta splendens''), commonly known as the betta, is a freshwater fish native to Southeast Asia, namely Cambodia, Laos, Myanmar, Malaysia, Indonesia, Thailand, and Vietnam. It is one of 76 species of the genus '' Betta,'' but the only one eponymously called "betta", owing to its global popularity as a pet; ''Betta splendens'' are among the most popular aquarium fish in the world, due to their diverse and colorful morphology and relatively low maintenance. Betta fish are endemic to the central plain of Thailand, where they were first domesticated at least 1,000 years ago, among the longest of any fish. They were initially bred for aggression and subject to gambling matches akin to cockfighting. Bettas became known outside Thailand through King Rama III (1788–1851), who is said to have given some to Theodore Cantor, a Danish physician, zoologist, and botanist. They first appeared in the West in the late 19th century, and within decades became popu ...
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