|
Western Blotting
The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the different proteins in a complicated protein combination. Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody to visualize. A synthetic or animal-derived antibody (known as the primary antibody) is created that recognizes and binds to a specific target protein. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognizes and binds to the primary antibo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Western Blot Workflow
Western may refer to: Places *Western, Nebraska, a village in the US *Western, New York, a town in the US *Western Creek, Tasmania, a locality in Australia *Western Junction, Tasmania, a locality in Australia *Western world, countries that identify with shared "Western" culture *Western United States, a region of the United States Arts and entertainment Films * ''Western'' (1997 film), a French road movie directed by Manuel Poirier * ''Western'' (2017 film), a German-Austrian film Genres *Western (genre), a category of fiction and visual art centered on the American Old West **Western fiction, the Western genre as featured in literature **Western film, the western genre in film **Western music (North America), a type of American folk music Music * ''Westerns'' (EP), an EP by Pete Yorn *WSTRN, a British hip hop group from west London *"Western" a song by Black Midi from ''Schlagenheim'' Business *The Western, a closed hotel/casino in Las Vegas, United States *Western Cartri ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Immunostaining
In biochemistry, immunostaining is any use of an antibody-based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods. Techniques Immunohistochemistry Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. While the first cases of IHC staining used fluorescent dyes (see ''immunofluorescence''), other non-fluorescent methods using enzymes such as peroxidase (see '' immunoperoxidase staining'') and alkaline phosphatase are now used. These enzymes are capable of catalysing reactions that give a coloured product that is easily detectable by ligh ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dilution Series
Dilution may refer to: * Reducing the concentration of a chemical * Serial dilution, stepwise * Homeopathic dilution * Dilution (equation), an equation to calculate the rate a gas dilutes *Trademark dilution, weakening of a trademark by unauthorised use *Stock dilution, issuing of new company shares *Dilution gene, lightening animal coat color * Dilution ratio * Hemodynamics#Hemodilution, of blood * Dilution refrigerator, cryogenic device See also * Delusion (other) * Dilation (other) Dilation (or dilatation) may refer to: Physiology or medicine * Cervical dilation, the widening of the cervix in childbirth, miscarriage etc. * Coronary dilation, or coronary reflex * Dilation and curettage, the opening of the cervix and surg ... {{disambig zh:稀释 nl:Verdunning ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Post-translational Modification
In molecular biology, post-translational modification (PTM) is the covalent process of changing proteins following protein biosynthesis. PTMs may involve enzymes or occur spontaneously. Proteins are created by ribosomes, which translation (biology), translate mRNA into polypeptide chains, which may then change to form the mature protein product. PTMs are important components in cell signal transduction, signalling, as for example when prohormones are converted to hormones. Post-translational modifications can occur on the amino acid side chains or at the protein's C-terminus, C- or N-terminus, N- termini. They can expand the chemical set of the 22 proteinogenic amino acid, amino acids by changing an existing functional group or adding a new one such as phosphate. Phosphorylation is highly effective for controlling the enzyme activity and is the most common change after translation. Many eukaryotic and prokaryotic proteins also have carbohydrate molecules attached to them in a pro ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biochemistry
Biochemistry, or biological chemistry, is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology, and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines. Almost all List of life sciences, areas of the life sciences are being uncovered and developed through biochemical methodology and research.#Voet, Voet (2005), p. 3. Biochemistry focuses on understanding the chemical basis that allows biomolecule, biological molecules to give rise to the processes that occur within living Cell (biology), cells and between cells,#Karp, Karp (2009), p. 2. in turn relating greatly to the understanding of tissue (biology), tissues and organ (anatomy), organs as well as organism structure and function.#Miller, Miller (2012). p. 62. Biochemistry is closely ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Western Blot Results For HIV Test
Western may refer to: Places *Western, Nebraska, a village in the US *Western, New York, a town in the US * Western Creek, Tasmania, a locality in Australia * Western Junction, Tasmania, a locality in Australia *Western world, countries that identify with shared "Western" culture *Western United States, a region of the United States Arts and entertainment Films * ''Western'' (1997 film), a French road movie directed by Manuel Poirier * ''Western'' (2017 film), a German-Austrian film Genres *Western (genre), a category of fiction and visual art centered on the American Old West **Western fiction, the Western genre as featured in literature **Western film, the western genre in film **Western music (North America), a type of American folk music Music * ''Westerns'' (EP), an EP by Pete Yorn * WSTRN, a British hip hop group from west London *"Western" a song by Black Midi from ''Schlagenheim'' Business * The Western, a closed hotel/casino in Las Vegas, United States *Western Ca ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
PubMed
PubMed is an openly accessible, free database which includes primarily the MEDLINE database of references and abstracts on life sciences and biomedical topics. The United States National Library of Medicine (NLM) at the National Institutes of Health maintains the database as part of the Entrez system of information retrieval. From 1971 to 1997, online access to the MEDLINE database was provided via computer and phone lines primarily through institutional facilities, such as university libraries. PubMed, first released in January 1996, ushered in the era of private, free, home- and office-based MEDLINE searching. The PubMed system was offered free to the public starting in June 1997. Content In addition to MEDLINE, PubMed provides access to: * older references from the print version of '' Index Medicus'', back to 1951 and earlier * references to some journals before they were indexed in Index Medicus and MEDLINE, for instance ''Science'', '' BMJ'', and ''Annals of Surg ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Switzerland
Switzerland, officially the Swiss Confederation, is a landlocked country located in west-central Europe. It is bordered by Italy to the south, France to the west, Germany to the north, and Austria and Liechtenstein to the east. Switzerland is geographically divided among the Swiss Plateau, the Swiss Alps, Alps and the Jura Mountains, Jura; the Alps occupy the greater part of the territory, whereas most of the country's Demographics of Switzerland, 9 million people are concentrated on the plateau, which hosts List of cities in Switzerland, its largest cities and economic centres, including Zurich, Geneva, and Lausanne. Switzerland is a federal republic composed of Cantons of Switzerland, 26 cantons, with federal authorities based in Bern. It has four main linguistic and cultural regions: German, French, Italian and Romansh language, Romansh. Although most Swiss are German-speaking, national identity is fairly cohesive, being rooted in a common historical background, shared ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Basel
Basel ( ; ), also known as Basle ( ), ; ; ; . is a city in northwestern Switzerland on the river Rhine (at the transition from the High Rhine, High to the Upper Rhine). Basel is Switzerland's List of cities in Switzerland, third-most-populous city (after Zurich and Geneva), with 177,595 inhabitants within the city municipality limits. The official language of Basel is Swiss Standard German and the main spoken language is the local Basel German dialect. Basel is commonly considered to be the cultural capital of Switzerland and the city is famous for its many Museums in Basel, museums, including the Kunstmuseum Basel, Kunstmuseum, which is the first collection of art accessible to the public in the world (1661) and the largest museum of Swiss art, art in Switzerland, the Fondation Beyeler (located in Riehen), the Museum Tinguely and the Museum of Contemporary Art (Basel), Museum of Contemporary Art, which is the first public museum of contemporary art in Europe. Forty museums ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Friedrich Miescher Institute For Biomedical Research
The Friedrich Miescher Institute for Biomedical Research (FMI) is a biomedical research institute founded in 1970. Based in Basel, Switzerland, the FMI is affiliated with the University of Basel and the Novartis Institutes for BioMedical Research (NIBR). It is named after Friedrich Miescher. , the FMI has around 340 collaborators, of which 20 are research group leaders, over 80 are postdoctoral collaborators and over 80 are postgraduate students participating in the FMI International PhD Program. The FMI is directed by Dirk Schübeler. The FMI is member of EU-LIFE, an alliance of leading life sciences research centres in Europe. Scientific activities The FMI is devoted to the pursuit of fundamental biomedical research. Areas of research are neurobiology, genome regulation, and multicellular systems. Research is carried out in 20 independent but highly interactive research groups. In addition, several cutting-edge technology platforms – including microscopy & imaging, comp ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
George Stark
George Stark (born 1933) is an American chemist and biochemist. His research interests include protein and enzyme function and modification, interferons and cytokines, signal transduction, and gene expression. Personal life George Stark was born in New York City in 1933. His father, Jack Stark, was a restaurant owner, and his mother, Florence Stark, was a bookkeeper. He was the youngest of three children, with two older sisters, Edna and Bernyce. Stark and his family moved to semi-rural Maryland (around the Washington, D.C. area) at the start of World War II. His father opened a restaurant named “Stark's Beef and Beans,” which Stark would often help at. The family remained there until Stark was through his third year of high school, after which they relocated back to New York City, NY in 1950. Stark would then finish his education in New York City. Over the course of his career, he relocated to various cities to conduct his research ranging from California to the United Kin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Northern Blot
The northern blot, or RNA blot,Gilbert, S. F. (2000) Developmental Biology, 6th Ed. Sunderland MA, Sinauer Associates. is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.Kevil, C. G., Walsh, L., Laroux, F. S., Kalogeris, T., Grisham, M. B., Alexander, J. S. (1997) An Improved, Rapid Northern Protocol. Biochem. and Biophys. Research Comm. 238:277–279. With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and morphogenesis, as well as in abnormal or diseased conditions. Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. Strictly speaking, the term 'northern blot' refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
