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Microtomy
A microtome (from the Greek ''mikros'', meaning "small", and ''temnein'', meaning "to cut") is a cutting tool used to produce extremely thin slices of material known as ''sections'', with the process being termed microsectioning. Important in science, microtomes are used in microscopy for the preparation of samples for observation under transmitted light or electron radiation. Microtomes use steel, glass or diamond blades depending upon the specimen being sliced and the desired thickness of the sections being cut. Steel blades are used to prepare histological sections of animal or plant tissues for light microscopy. Glass knives are used to slice sections for light microscopy and to slice very thin sections for electron microscopy. Industrial grade diamond knives are used to slice hard materials such as bone, teeth and tough plant matter for both light microscopy and for electron microscopy. Gem-quality diamond knives are also used for slicing thin sections for electron microsc ...
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Diamond Knife
A diamond knife is a very sharp knife in which the edge is made from diamond, invented by Humberto Fernández-Morán in 1955. Diamond knives are used for medical and scientific applications where an extremely sharp and long-lasting edge is essential. The knives are very expensive to purchase, depending on the quality and size of the knife; in addition the knives must be professionally sharpened as the edge dulls. Eye surgery Diamond knives are used in eye surgery, specifically in refractive surgery. In particular they are the main tool, together with the microscope, for the radial keratotomy invented by Svyatoslav Fyodorov to correct myopia and for the Mini Asymmetric Radial Keratotomy (M.A.R.K.), invented by Marco Abbondanza to correct astigmatism and cure the first and second stages of keratoconus. Ultramicrotomy Metal microtome knives or razor blades are too soft and dull to cut ultrathin sections. In 1950, Latta and Hartmann discovered that the edge of broken glass could b ...
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Histological
Histology, also known as microscopic anatomy or microanatomy, is the branch of biology that studies the microscopic anatomy of biological tissue (biology), tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope. Although one may divide microscopic anatomy into ''organology'', the study of organs, ''histology'', the study of tissues, and ''cytology'', the study of cell (biology), cells, modern usage places all of these topics under the field of histology. In medicine, histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue. In the field of paleontology, the term paleohistology refers to the histology of fossil organisms. Biological tissues Animal tissue classification There are four basic types of animal tissues: muscle tissue, nervous tissue, connective tissue, and epithelial tissue. All animal tissues are considered to be subtypes of these ...
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Electron Microscopy
An electron microscope is a microscope that uses a beam of electrons as a source of illumination. It uses electron optics that are analogous to the glass lenses of an optical light microscope to control the electron beam, for instance focusing it to produce magnified images or electron diffraction patterns. As the wavelength of an electron can be up to 100,000 times smaller than that of visible light, electron microscopes have a much higher resolution of about 0.1 nm, which compares to about 200 nm for light microscopes. ''Electron microscope'' may refer to: * Transmission electron microscope (TEM) where swift electrons go through a thin sample * Scanning transmission electron microscope (STEM) which is similar to TEM with a scanned electron probe * Scanning electron microscope (SEM) which is similar to STEM, but with thick samples * Electron microprobe similar to a SEM, but more for chemical analysis * Low-energy electron microscope (LEEM), used to image surfaces * ...
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Wilhelm His, Sr
Wilhelm may refer to: People and fictional characters * William Charles John Pitcher, costume designer known professionally as "Wilhelm" * Wilhelm (name), a list of people and fictional characters with the given name or surname Other uses * Wilhelm (name), disambiguation page for people named Wilhelm ** Wilhelm II (1858–1941), king of Prussia and emperor of Germany from 1888 until his abdication in 1918. * Mount Wilhelm, the highest mountain in Papua New Guinea * Wilhelm Archipelago, Antarctica * Wilhelm (crater), a lunar crater * Wilhelm scream, stock sound effect used in many movies and shows See also * Wilhelm scream, a stock sound effect * SS ''Kaiser Wilhelm II'', or USS ''Agamemnon'', a German steam ship * Wilhelmus, the Dutch national anthem * William Helm William Helm (March 9, 1837 – April 10, 1919) was an American Sheep-rearing, sheep farmer and among the early pioneer settlers of Fresno County, California, Fresno County, California. He was instrumental in t ...
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Cummings 1774 Microtome
Cummings may refer to: Places Canada * Cummings, Saskatchewan, an unincorporated hamlet United States * Cummings, Mendocino County, California, an unincorporated community * Cummings, Kansas * Cummings, North Dakota, an unincorporated community * Cummings Research Park, Huntsville, Alabama * Cummings Township, Lycoming County, Pennsylvania * Cummings Mountain (other) Other uses * Cummings (surname) * USS ''Cummings'', two United States Navy destroyers * Cummings Jewish Centre for Seniors, in Montreal, Quebec, Canada * Walt Cummings, a fictional character in the TV series ''24'' * Cummings, a character in ''The Diary of a Nobody'' by George and Weedon Grossmith See also * Cummings House (other) * Cumming (other) * Cummins (other) Cummins is a manufacturer of diesel and natural gas engines. Cummins may also refer to: Places * Cummins, South Australia, a town on the Eyre Peninsula in South Australia * Cummins Creek Wilderness, on the ...
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Light Microscope
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible spectrum, visible light and a system of lens (optics), lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve optical resolution, resolution and sample contrast (vision), contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope. In high-power microscopes, both eyepieces typically show the same image, but with a stereo microscope, slightly different images are used to create a 3-D effect. A camera is typically used to capture the image (micrograph). The sample can be lit in a variety of ways. Transparent objects can be lit from below and solid objects can be lit with light coming through ...
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Alexander Cummings
Alexander Cumming FRSE (sometimes referred to as Alexander Cummings; 1733 – 8 March 1814) was a Scottish watchmaker and instrument inventor, who was the first to patent a design of the flush toilet in 1775, which had been pioneered by Sir John Harington, but without solving the problem of foul smells. As well as improving the flush mechanism, Cummings included an S-trap (or bend) to retain water permanently within the waste pipe, thus preventing sewer gases from entering buildings. Most modern flush toilets still include a similar trap. Early life Cumming was a mathematician and mechanic as well as a watchmaker. Little is known of his early life. He was born in Edinburgh in 1733, the son of James Cumming of Duthil. He is recorded as having been apprenticed to an Edinburgh watchmaker. Career In the 1750s he was employed by Archibald Campbell, 3rd Duke of Argyll, at Inverary as an organ builder as well as a clockmaker. After his move to England he continued to work in bot ...
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Jan Evangelista Purkyně
Jan Evangelista Purkyně (; also written Johann Evangelist Purkinje) (17 or 18 December 1787 – 28 July 1869) was a Czech anatomist and physiologist. In 1839, he coined the term " protoplasma" for the fluid substance of a cell. He was one of the best known scientists of his time. Such was his fame that when people from outside Europe wrote letters to him, all that they needed to put as the address was "Purkyně, Europe". Biography Purkyně was born in the Kingdom of Bohemia (then part of the Austrian monarchy, now Czech Republic). After completing senior high school in 1804, Purkyně joined the Piarists order as a monk but subsequently left "to deal more freely with science." In 1818, Purkyně graduated from Charles University in Prague with a degree in medicine, where he was appointed a Professor of Physiology. He discovered the Purkinje effect, the human eye's much reduced sensitivity to dim red light compared to dim blue light, and published in 1823 description of several ...
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Nanometre
330px, Different lengths as in respect to the Molecule">molecular scale. The nanometre (international spelling as used by the International Bureau of Weights and Measures; SI symbol: nm), or nanometer (American spelling), is a unit of length in the International System of Units (SI), equal to one billionth ( short scale) or one thousand million (long scale) of a meter (0.000000001 m) and to 1000  picometres. One nanometre can be expressed in scientific notation as 1 × 10−9 m and as  m. History The nanometre was formerly known as the "''millimicrometre''" – or, more commonly, the "''millimicron''" for short – since it is of a micrometer. It was often denoted by the symbol ''mμ'' or, more rarely, as ''μμ'' (however, ''μμ'' should refer to a ''millionth'' of a micron). Etymology The name combines the SI prefix '' nano-'' (from the Ancient Greek , ', "dwarf") with the parent unit name ''metre'' (from Greek , ', "unit of measurement"). ...
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Paraffin Wax
Paraffin wax (or petroleum wax) is a soft colorless solid derived from petroleum, coal, or oil shale that consists of a mixture of hydrocarbon molecules containing between 20 and 40 carbon atoms. It is solid at room temperature and melting point, begins to melt above approximately , and its boiling point is above . Common applications for paraffin wax include lubrication, electrical insulation, and candles; dyed paraffin wax can be made into crayons. Un-dyed, unscented paraffin candles are odorless and bluish-white. Paraffin wax was first created by Carl Reichenbach#Scientific contributions, Carl Reichenbach in Germany in 1830 and marked a major advancement in candlemaking technology, as it burned more cleanly and reliably than tallow candles and was cheaper to produce. In chemistry, ''paraffin'' is used synonymously with ''alkane'', indicating hydrocarbons with the general formula C''n''H2''n''+2. The name is derived from Latin ''parum'' ("very little") + ''affinis'', meaning ...
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