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Micropropagation
Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods. It is also used to provide a sufficient number of plantlets for planting from seedless plants, plants that do not respond well to vegetative reproduction or where micropropagation is the cheaper means of propagating (e.g. Orchids). Cornell University botanist Frederick Campion Steward discovered and pioneered micropropagation and plant tissue culture in the late 1950s and early 1960s. Steps In short, steps of micropropagation can be divided into four stages: # Selection of mother plant # Multiplication # Rooting and acclimatizing # Transfer new plant to soil Selection of mother plant Micropropagation begins with the selection of plant m ...
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Callus (cell Biology)
Plant callus (plural ''calluses'' or ''calli'') is a growing mass of unorganized plant Ground tissue, parenchyma cells. In living plants, callus cells are those cells that cover a plant wound. In biological research and biotechnology callus formation is induced from plant tissue samples (explants) after surface sterilization and plating onto tissue culture medium in vitro (in a closed culture vessel such as a Petri dish). The culture medium is supplemented with Plant hormone, plant growth regulators, such as auxin, cytokinin, and gibberellin, to initiate callus formation or Somatic Embryogenesis, somatic embryogenesis. Callus initiation has been described for all major groups of land plants. Callus induction and tissue culture Plant species representing all major land plant groups have been shown to be capable of producing callus in tissue culture. A callus cell culture is usually sustained on gel medium. Callus induction medium consists of agar and a mixture of macronutri ...
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Plant Tissue Culture
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including: * The production of exact copies of plants that produce particularly good flowers, fruits, or other desirable traits. * To quickly produce mature plants. * To produce a large number of plants in a reduced space. * The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds. * The regeneration of whole plants from plant cells that have been genetically modified. * The production of plants in sterile containers allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens. * The production of plants from seeds tha ...
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Coconut
The coconut tree (''Cocos nucifera'') is a member of the palm tree family (biology), family (Arecaceae) and the only living species of the genus ''Cocos''. The term "coconut" (or the archaic "cocoanut") can refer to the whole coconut palm, the seed, or the fruit, which botanically is a drupe, not a Nut (fruit), nut. Originally native to Central Indo-Pacific, they are now ubiquitous in coastal tropical regions and are a cultural icon of the tropics. The coconut tree provides food, fuel, cosmetics, folk medicine and building materials, among many other uses. The inner flesh of the mature seed, as well as the coconut milk extracted from it, forms a regular part of the diets of many people in the tropics and subtropics. Coconuts are distinct from other fruits because their endosperm contains a large quantity of an almost clear liquid, called "coconut water" or "coconut juice". Mature, ripe coconuts can be used as edible seeds, or processed for Coconut oil, oil and Coconut milk, ...
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Simple Micropropagation Technique
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Differentiate (biology)
Cellular differentiation is the process in which a stem cell changes from one type to a differentiated one. Usually, the cell changes to a more specialized type. Differentiation happens multiple times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Metabolic composition, however, gets dramatically altered where stem cell ...
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Sugarcane
Sugarcane or sugar cane is a species of tall, Perennial plant, perennial grass (in the genus ''Saccharum'', tribe Andropogoneae) that is used for sugar Sugar industry, production. The plants are 2–6 m (6–20 ft) tall with stout, jointed, fibrous stalks that are rich in sucrose, which accumulates in the Plant stem, stalk internodes. Sugarcanes belong to the grass family, Poaceae, an economically important flowering plant family that includes maize, wheat, rice, and sorghum, and many forage crops. It is native to New Guinea. Sugarcane was an ancient crop of the Austronesian people, Austronesian and Indigenous people of New Guinea, Papuan people. The best evidence available today points to the New Guinea area as the site of the original domestication of ''Saccharum officinarum''. It was introduced to Polynesia, Island Melanesia, and Madagascar in prehistoric times via Austronesian sailors. It was also introduced by Austronesian sailors to India and then to Southern China by 500 ...
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Strawberry
The garden strawberry (or simply strawberry; ''Fragaria × ananassa'') is a widely grown Hybrid (biology), hybrid plant cultivated worldwide for its fruit. The genus ''Fragaria'', the strawberries, is in the rose family, Rosaceae. The fruit is appreciated for its aroma, bright red colour, juicy texture, and sweetness. It is eaten either fresh or in prepared foods such as fruit preserves, jam, ice cream, and chocolates. Artificial strawberry flavourings and aromas are widely used in commercial products. Botanically, the strawberry is not a berry (botany), berry, but an aggregate fruit, aggregate accessory fruit, accessory fruit. Each apparent 'seed' on the outside of the strawberry is actually an achene, a botanical fruit with a seed inside it. The garden strawberry was first bred in Brittany, France, in the 1750s via a cross of ''Virginia strawberry, F. virginiana'' from eastern North America and ''Fragaria chiloensis, F. chiloensis'', which was brought from Chile by Amédé ...
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Primordium
A primordium (; : primordia; synonym: anlage), in embryology, is an Organ (anatomy), organ or tissue in its earliest recognizable stage of development. Cell (biology), Cells of the primordium are called primordial cells. A primordium is the simplest set of cells capable of triggering growth of the would-be organ and the initial foundation from which an organ is able to grow. In flowering plants, a floral primordium gives rise to a flower. Although it is a frequently used term in plant biology, the word is used in describing the biology of all multicellular organisms (for example: a tooth primordium in animals, a leaf primordium in plants or a sporocarp (fungi), sporophore primordium in fungi.) Primordium development in plants Plants produce both leaf and flower primordia cells at the shoot apical meristem (SAM). Primordium development in plants is critical to the proper positioning and development of plant organs and cells. The process of primordium development is intricately r ...
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Meristem
In cell biology, the meristem is a structure composed of specialized tissue found in plants, consisting of stem cells, known as meristematic cells, which are undifferentiated cells capable of continuous cellular division. These meristematic cells play a fundamental role in plant growth, regeneration, and acclimatization, as they serve as the source of all differentiated plant tissues and organs. They contribute to the formation of structures such as fruits, leaves, and seeds, as well as supportive tissues like stems and roots. Meristematic cells are totipotent, meaning they have the ability to differentiate into any plant cell type. As they divide, they generate new cells, some of which remain meristematic cells while others differentiate into specialized cells that typically lose the ability to divide or produce new cell types. Due to their active division and undifferentiated nature, meristematic cells form the foundation for the formation of new plant organs and the c ...
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Plant Tissue Cultures, National Center For Genetic Resources Preservation, USDA
Plants are the eukaryotes that form the kingdom Plantae; they are predominantly photosynthetic. This means that they obtain their energy from sunlight, using chloroplasts derived from endosymbiosis with cyanobacteria to produce sugars from carbon dioxide and water, using the green pigment chlorophyll. Exceptions are parasitic plants that have lost the genes for chlorophyll and photosynthesis, and obtain their energy from other plants or fungi. Most plants are multicellular, except for some green algae. Historically, as in Aristotle's biology, the plant kingdom encompassed all living things that were not animals, and included algae and fungi. Definitions have narrowed since then; current definitions exclude fungi and some of the algae. By the definition used in this article, plants form the clade Viridiplantae (green plants), which consists of the green algae and the embryophytes or land plants (hornworts, liverworts, mosses, lycophytes, ferns, conifers and other gymnosperm ...
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Weaning
Weaning is the process of gradually introducing an infant human or other mammal to what will be its adult diet while withdrawing the supply of its mother's milk. In the United Kingdom, UK, weaning primarily refers to the introduction of solid foods at 6 months; in the United States, US, it primarily refers to stopping breastfeeding. The process takes place only in mammals, as only mammals produce milk. The infant is considered to be fully weaned once it is no longer breastfeeding, fed by any breast milk (or bottled Infant formula, substitute). Humans In some cultures, weaning progresses with the introduction of feeding the child food that has been prechewed by the parent along with continued breastfeeding, a practice known as premastication. The practice was important throughout human history in that it naturally gave a child a greatly improved protein source in addition to preventing iron deficiency. However, premasticated food from caregivers of lower socioeconomic status i ...
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Stomata
In botany, a stoma (: stomata, from Greek ''στόμα'', "mouth"), also called a stomate (: stomates), is a pore found in the epidermis of leaves, stems, and other organs, that controls the rate of gas exchange between the internal air spaces of the leaf and the atmosphere. The pore is bordered by a pair of specialized parenchyma cells known as guard cells that regulate the size of the stomatal opening. The term is usually used collectively to refer to the entire stomatal complex, consisting of the paired guard cells and the pore itself, which is referred to as the stomatal aperture. Air, containing oxygen, which is used in respiration, and carbon dioxide, which is used in photosynthesis, passes through stomata by gaseous diffusion. Water vapour diffuses through the stomata into the atmosphere as part of a process called transpiration. Stomata are present in the sporophyte generation of the vast majority of land plants, with the exception of liverworts, as well as so ...
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