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De Novo Peptide Sequencing
In mass spectrometry, de novo peptide sequencing is the method in which a peptide amino acid sequence is determined from tandem mass spectrometry. Knowing the amino acid sequence of peptides from a protein digest is essential for studying the biological function of the protein. In the old days, this was accomplished by the Edman degradation procedure. Today, analysis by a tandem mass spectrometer is a more common method to solve the sequencing of peptides. Generally, there are two approaches: database search and de novo sequencing. Database search is a simple version as the mass spectra data of the unknown peptide is submitted and run to find a match with a known peptide sequence, the peptide with the highest matching score will be selected. This approach fails to recognize novel peptides since it can only match to existing sequences in the database. De novo sequencing is an assignment of fragment ions from a mass spectrum. Different algorithms are used for interpretation and most i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mass Spectrometry
Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio of ions. The results are presented as a ''mass spectrum'', a plot of intensity as a function of the mass-to-charge ratio. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures. A mass spectrum is a type of plot of the ion signal as a function of the mass-to-charge ratio. These spectra are used to determine the elemental or isotopic signature of a sample, the masses of particles and of molecules, and to elucidate the chemical identity or structure of molecules and other chemical compounds. In a typical MS procedure, a sample, which may be solid, liquid, or gaseous, is ionization, ionized, for example by bombarding it with a Electron ionization, beam of electrons. This may cause some of the sample's molecules to break up into positively charged fragments or simply become positively charged without fragmenting. These ions (fragmen ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Satellite Ions
A satellite or an artificial satellite is an object, typically a spacecraft, placed into orbit around a Astronomical object, celestial body. They have a variety of uses, including communication relay, weather forecasting, navigation (GPS), broadcasting, scientific research, and Earth observation. Additional military uses are reconnaissance, Early warning system, early warning, signals intelligence and, potentially, weapon delivery. Other satellites include the final rocket stages that place satellites in orbit and formerly useful satellites that later become defunct. Except for passive satellites, most satellites have an electricity generation system for equipment on board, such as solar panels or radioisotope thermoelectric generators (RTGs). Most satellites also have a method of communication to ground stations, called Transponder (satellite communications), transponders. Many satellites use a Satellite bus, standardized bus to save cost and work, the most popular of which a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Time-of-flight
Time of flight (ToF) is the measurement of the time taken by an object, particle or wave (be it acoustic, electromagnetic, etc.) to travel a distance through a medium. This information can then be used to measure velocity or path length, or as a way to learn about the particle or medium's properties (such as composition or flow rate). The traveling object may be detected directly (direct time of flight, dToF, e.g., via an ion detector in mass spectrometry) or indirectly (indirect time of flight, iToF, e.g., by light scattered from an object in laser doppler velocimetry). Time of flight technology has found valuable applications in the monitoring and characterization of material and biomaterials, hydrogels included. Overview In electronics, one of the earliest devices using the principle are ultrasonic distance-measuring devices, which emit an ultrasonic pulse and are able to measure the distance to a solid object based on the time taken for the wave to bounce back to the emitter. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Quadrupole
A quadrupole or quadrapole is one of a sequence of configurations of things like electric charge or current, or gravitational mass that can exist in ideal form, but it is usually just part of a multipole expansion of a more complex structure reflecting various orders of complexity. Mathematical definition The quadrupole moment tensor ''Q'' is a rank-two tensor—3×3 matrix. There are several definitions, but it is normally stated in the traceless form (i.e. Q_ + Q_ + Q_ = 0). The quadrupole moment tensor has thus nine components, but because of transposition symmetry and zero-trace property, in this form only five of these are independent. For a discrete system of \ell point charges or masses in the case of a gravitational quadrupole, each with charge q_\ell, or mass m_\ell, and position \mathbf_\ell = \left(r_, r_, r_\right) relative to the coordinate system origin, the components of the ''Q'' matrix are defined by: Q_ = \sum_\ell q_\ell\left(3r_ r_ - \left\, \mathbf_\el ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
PEAKS
PEAKS is a proteomics software program for tandem mass spectrometry designed for peptide sequencing, protein identification and quantification. Description PEAKS is commonly used for peptide identification (Protein ID) through de novo peptide sequencing assisted search engine A search engine is a software system that provides hyperlinks to web pages, and other relevant information on World Wide Web, the Web in response to a user's web query, query. The user enters a query in a web browser or a mobile app, and the sea ... database searching. PEAKS has also integrated PTM and mutation characterization through automatic peptide sequence tag based searching (SPIDER)Ma B, Johnson. De Novo sequencing and homology searching Molecular & Cellular Proteomics. 10.1074/mcp.O111.014902 (2011). and PTM Identification. PEAKS provides a complete sequence for each peptide, confidence scores on individual amino acid assignments, simple reporting for high-throughput analysis, amongst other i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
State-of-the-art
The state of the art (SOTA or SotA, sometimes cutting edge, leading edge, or bleeding edge) refers to the highest level of general development, as of a device, technique, or scientific field achieved at a particular time. However, in some contexts it can also refer to a level of development reached at any particular time as a result of the common methodologies employed at the time. The term has been used since 1910, and has become both a common term in advertising and marketing, and a legally significant phrase with respect to both patent law and tort liability. In advertising, the phrase is often used to convey that a product is made with the best or latest available technology, but it has been noted that "the term 'state of the art' requires little proof on the part of advertisers", as it is considered mere puffery. The use of the term in patent law "does not connote even superiority, let alone the superlative quality the ad writers would have us ascribe to the term". Or ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Masses Of B2-ions
Mass is the quantity of matter in a physical body and a measure of the body's inertia. Mass or may also refer to: Arts, entertainment, and media Music *Mass (music), a choral composition that sets liturgical text to music * ''Mass'' (Stravinsky), a composition by Igor Stravinsky * ''Mass'' (Bernstein), a musical theater work by Leonard Bernstein * Mass (English band), a post-punk band * ''Mass'' (Grotus album), 1996 * ''Mass'' (Alastair Galbraith album), 2011 * ''Mass'' (The Gazette album), 2021 * ''The Mass'' (album), by musical project Era Other uses in arts, entertainment, and media * ''Mass'' (2004 film), Indian Telugu-language film * ''Mass'', Hindi title of the 2015 Indian Tamil-language film ''Massu Engira Masilamani'' * ''Mass'' (2021 film), American drama film * ''Mass'' (novel), a 1973 novel by Filipino author F. Sionil José * Mass media, communication channels which can reach huge numbers of people * '' The Masses'', a socialist magazine published in the US from 1 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Amino Acid Fragment Ions
In chemistry, amines (, ) are organic compounds that contain carbon-nitrogen bonds. Amines are formed when one or more hydrogen atoms in ammonia are replaced by alkyl or aryl groups. The nitrogen atom in an amine possesses a lone pair of electrons. Amines can also exist as hetero cyclic compounds. Aniline is the simplest aromatic amine, consisting of a benzene ring bonded to an amino group. Amines are classified into three types: primary (1°), secondary (2°), and tertiary (3°) amines. Primary amines (1°) contain one alkyl or aryl substituent and have the general formula RNH2. Secondary amines (2°) have two alkyl or aryl groups attached to the nitrogen atom, with the general formula R2NH. Tertiary amines (3°) contain three substituent groups bonded to the nitrogen atom, and are represented by the formula R3N. The functional group present in primary amines is called the amino group. Classification of amines Amines can be classified according to the nature and number o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
MALDI
In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy-absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and carbohydrates) and various organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft (low fragmentation) ways of obtaining ions of large molecules in the gas phase, though MALDI typically produces far fewer multi-charged ions . MALDI methodology is a three-step process. First, the sample is mixed with a suitable matrix material and applied to a metal plate. Second, a pulsed laser irradiates the sample, triggering ablation and desorption of the sample and matrix materi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electron Capture Dissociation
Electron-capture dissociation (ECD) is a method of fragmenting gas-phase ions for structure elucidation of peptides and proteins in tandem mass spectrometry. It is one of the most widely used techniques for activation and dissociation of mass selected precursor ion in MS/MS. It involves the direct introduction of low-energy electrons to trapped gas-phase ions. History Electron-capture dissociation was developed by Roman Zubarev and Neil Kelleher while in Fred McLafferty's lab at Cornell University. Irradiation of melittin 4+ ions and ubiquitin 10+ ions (trapped in FT-MS cell) by laser pulses not only resulted in peculiar c', z fragmentation but also charge reduction. It was suggested that if FT cell is modified to trap cations and electrons simultaneously, secondary electrons emitted by UV photons increases the charge reduction effect and c′, z• fragmentation. Replacing UV laser with EI source led to the development of this new technique. Principles Electron-capture d ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electron Transfer Dissociation
Electron-transfer dissociation (ETD) is a method of fragmenting multiply-charged gaseous macromolecules in a mass spectrometer between the stages of tandem mass spectrometry (MS/MS). Similar to electron-capture dissociation, ETD induces fragmentation of large, multiply-charged cations by transferring electrons to them. ETD is used extensively with polymers and biological molecules such as proteins and peptides for sequence analysis. Transferring an electron causes peptide backbone cleavage into c- and z-ions while leaving labile post translational modifications (PTM) intact. The technique only works well for higher charge state peptide or polymer ions (z>2). However, relative to collision-induced dissociation (CID), ETD is advantageous for the fragmentation of longer peptides or even entire proteins. This makes the technique important for top-down proteomics. The method was developed by Hunt and coworkers at the University of Virginia. History Electron-capture dissociation ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Collision-induced Dissociation
Collision-induced dissociation (CID), also known as collisionally activated dissociation (CAD), is a mass spectrometry technique to induce fragmentation (chemistry), fragmentation of selected ions in the gas phase. The selected ions (typically molecular ions or protonated molecules) are usually accelerated by applying an electrical potential to increase the ion kinetic energy and then allowed to collide with neutral molecules (often helium, nitrogen, or argon). In the collision, some of the kinetic energy is converted into internal energy which results in bond breakage and the fragmentation of the molecular ion into smaller fragments. These fragment ions can then be analyzed by tandem mass spectrometry. CID and the fragment ions produced by CID are used for several purposes. Partial or complete structural determination can be achieved. In some cases, identity can be established based on previous knowledge without determining structure. Another use is in simply achieving more se ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
