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Citrus Canker
Citrus canker is a disease affecting ''Citrus'' species caused by the bacterium '' Xanthomonas citri''. Infection causes lesions on the leaves, stems, and fruit of citrus trees, including lime, oranges, and grapefruit. While not harmful to humans, canker significantly affects the vitality of citrus trees, causing leaves and fruit to drop prematurely; a fruit infected with canker is safe to eat, but too unsightly to be sold. Citrus canker is mainly a leaf-spotting and rind-blemishing disease, but when conditions are highly favorable, it can cause defoliation, shoot dieback, and fruit drop. The disease, which is believed to have originated in Southeast Asia, is extremely persistent when it becomes established in an area. Citrus groves have been destroyed in attempts to eradicate the disease. Countries like Brazil and the United States also suffer from canker outbreaks. Biology ''Xanthomonas citri'' is a rod-shaped Gram-negative bacterium with polar flagella. The bacterium ha ...
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Citrus
''Citrus'' is a genus of flowering trees and shrubs in the family Rutaceae. Plants in the genus produce citrus fruits, including important crops such as oranges, mandarins, lemons, grapefruits, pomelos, and limes. ''Citrus'' is native to South Asia, East Asia, Southeast Asia, Melanesia, and Australia. Indigenous people in these areas have used and domesticated various species since ancient times. Its cultivation first spread into Micronesia and Polynesia through the Austronesian expansion (–1500 BCE). Later, it was spread to the Middle East and the Mediterranean () via the incense trade route, and from Europe to the Americas. Renowned for their highly fragrant aromas and complex flavor, citrus are among the most popular fruits in cultivation. With a propensity to hybridize between species, making their taxonomy complicated, there are numerous varieties encompassing a wide range of appearance and fruit flavors. Evolution Evolutionary history The large cit ...
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Stomata
In botany, a stoma (: stomata, from Greek ''στόμα'', "mouth"), also called a stomate (: stomates), is a pore found in the epidermis of leaves, stems, and other organs, that controls the rate of gas exchange between the internal air spaces of the leaf and the atmosphere. The pore is bordered by a pair of specialized parenchyma cells known as guard cells that regulate the size of the stomatal opening. The term is usually used collectively to refer to the entire stomatal complex, consisting of the paired guard cells and the pore itself, which is referred to as the stomatal aperture. Air, containing oxygen, which is used in respiration, and carbon dioxide, which is used in photosynthesis, passes through stomata by gaseous diffusion. Water vapour diffuses through the stomata into the atmosphere as part of a process called transpiration. Stomata are present in the sporophyte generation of the vast majority of land plants, with the exception of liverworts, as well as so ...
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Key Lime
The Key lime or acid lime (''Citrus'' × ''aurantiifolia'' or ''C. aurantifolia'') is a citrus hybrid (''kaffir lime, C. hystrix'' × ''citron, C. medica'') native to tropical Southeast Asia. It has a spherical fruit, in diameter. The Key lime is usually picked while it is still green, but it becomes yellow when ripe. The Key lime has thinner Peel (fruit), rind and is smaller, seedier, more acidic and more aromatic than the Persian lime (''Citrus × latifolia''). It is valued for its characteristic flavor. The name comes from its association with the Florida Keys, where it is best known as the flavoring ingredient in Key lime pie. The key lime is not to be confused with West Indian lime, bartender's lime, Omani lime, or Mexican lime which are slightly different. The last is classified as a distinct Race (biology), race with a thicker skin and darker green colour. Philippine varieties have various names, including ''dayap'' and ''bilolo''. Etymology The English word ''lime'' ...
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Grapefruit
The grapefruit (''Citrus'' × ''paradisi'') is a subtropical citrus tree known for its relatively large, sour to semi-sweet, somewhat bitter fruit. The flesh of the fruit is segmented and varies in color from pale yellow to dark red. Grapefruits originated in Barbados in the 18th century. They are a citrus hybrid that was created through an accidental cross between the sweet orange (''C.'' × ''sinensis'') and the pomelo (''C. maxima''), both of which were introduced to the Caribbean from Asia in the 17th century. It has also been called the 'forbidden fruit'. In the past it was called the ''pomelo'', but that term is now mostly used as the common name for ''Citrus maxima''. Grapefruit–drug interactions are common, as the juice contains furanocoumarins that interfere with the metabolism of many drugs. This can prolong and intensify the effects of those drugs, leading to multiple side-effects such as abnormal heart rhythms, bleeding inside the stomach, low blood pressure ...
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Bacteria
Bacteria (; : bacterium) are ubiquitous, mostly free-living organisms often consisting of one Cell (biology), biological cell. They constitute a large domain (biology), domain of Prokaryote, prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit the air, soil, water, Hot spring, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria play a vital role in many stages of the nutrient cycle by recycling nutrients and the nitrogen fixation, fixation of nitrogen from the Earth's atmosphere, atmosphere. The nutrient cycle includes the decomposition of cadaver, dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, suc ...
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Fungus
A fungus (: fungi , , , or ; or funguses) is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and mold (fungus), molds, as well as the more familiar mushrooms. These organisms are classified as one of the kingdom (biology)#Six kingdoms (1998), traditional eukaryotic kingdoms, along with Animalia, Plantae, and either Protista or Protozoa and Chromista. A characteristic that places fungi in a different kingdom from plants, bacteria, and some protists is chitin in their cell walls. Fungi, like animals, are heterotrophs; they acquire their food by absorbing dissolved molecules, typically by secreting digestive enzymes into their environment. Fungi do not photosynthesize. Growth is their means of motility, mobility, except for spores (a few of which are flagellated), which may travel through the air or water. Fungi are the principal decomposers in ecological systems. These and other differences place fungi in a single group of related o ...
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Clara H
Clara may refer to: Arts and entertainment * ''Clara'' (2018 film), a Canadian sci-fi drama * ''Clara'' (2019 film), a Ukrainian animated fantasy film * ''Clara'' (TV series), a German TV series * Clara the Cow, mascot of the Greek TV show '' Pio Poli Tin Kiriaki'' * ''Clara'' (opera), a 1998 opera by Hans Gefors, libretto by Jean-Claude Carrière * ''Clara'', a 1987 one-act stage play by Arthur Miller * ''Clara'' (album), a 2021 album by Loscil * "Clara", a song by Scott Walker from the 2006 album '' The Drift'' People and fictional characters * Clara (given name), a feminine given name, including a list of people and fictional characters * Clara (surname), a list of people surnamed Clara or Clarà * Clare of Assisi (1194–1253), sometimes called Clara, Italian saint * Aemilia Clara, mother of Roman Emperor Didius Julianus (emperor from March to June 193) * Didia Clara (born c. 153), only child of the Roman Emperor Didius Julianus and Empress Manlia Scantilla * Clara La ...
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Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation. Mullis and biochemist Michael Smith (chemist), Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of Ancient DNA, ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications including biomedical research and forensic ...
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Auxin
Auxins (plural of auxin ) are a class of plant hormones (or plant-growth regulators) with some morphogen-like characteristics. Auxins play a cardinal role in coordination of many growth and behavioral processes in plant life cycles and are essential for plant body development. The Dutch biologist Frits Warmolt Went first described auxins and their role in plant growth in the 1920s. Kenneth V. Thimann became the first to isolate one of these phytohormones and to determine its chemical structure as indole-3-acetic acid (IAA). Went and Thimann co-authored a book on plant hormones, ''Phytohormones'', in 1937. Overview Auxins were the first of the major plant hormones to be discovered. They derive their name from the Greek word ( – 'to grow/increase'). Auxin is present in all parts of a plant, although in very different concentrations. The concentration in each position is crucial developmental information, so it is subject to tight regulation through both metabolism and transp ...
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Gibberellin
Gibberellins (GAs) are plant hormones that regulate various Biological process, developmental processes, including Plant stem, stem elongation, germination, dormancy, flowering, flower development, and leaf and fruit senescence. They are one of the longest-known classes of plant hormone. It is thought that the selective breeding (albeit unconscious) of crop strains that were deficient in GA synthesis was one of the key drivers of the "Green Revolution, green revolution" in the 1960s, a revolution that is credited to have saved over a billion lives worldwide. Chemistry All known gibberellins are Diterpene#Diterpenoids, diterpenoid acids synthesized by the terpenoid pathway in plastids and then modified in the endoplasmic reticulum and cytosol until they reach their biologically active form. All are derived via the ''ent''-gibberellane skeleton but are synthesised via ''ent''-kaurene. The gibberellins are named GA1 through GAn in order of discovery. Gibberellic acid, which was the fi ...
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Type Three Secretion System
The type III secretion system (T3SS or TTSS) is one of the bacterial secretion systems used by bacteria to secretion, secrete their Bacterial effector protein, effector proteins into the host's cells to promote virulence and Colonisation (biology), colonisation. While the type III secretion system has been widely regarded as equivalent to the injectisome, many argue that the injectisome is only part of the type III secretion system, which also include structures like the flagellar export apparatus. The T3SS is a needle-like protein complex found in several species of pathogenic bacteria, pathogenic gram-negative bacteria. Overview The term '''Type III secretion system was coined in 1993. This secretion system is distinguished from at least five other secretion, secretion systems found in gram-negative bacteria. Many animal and plant associated bacteria possess similar T3SSs. These T3SSs are similar as a result of convergent evolution and phylogenetic analysis supports a model i ...
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Transcription Activator-like Effector
TAL (transcription activator-like) effectors (often referred to as TALEs, but not to be confused with the three amino acid loop extension homeobox class of proteins) are proteins secreted by some β- and γ-proteobacteria. Most of these are Xanthomonads. Plant pathogenic ''Xanthomonas'' bacteria are especially known for TALEs, produced via their type III secretion system. These proteins can bind promoter sequences in the host plant and activate the expression of plant genes that aid bacterial infection. The TALE domain responsible for binding to DNA is known to have 1.5 to 33.5 short sequences that are repeated multiple times (tandem repeats). Each of these repeats was found to be specific for a certain base pair of the DNA. These repeats also have repeat variable residues (RVD) that can detect specific DNA base pairs. They recognize plant DNA sequences through a central repeat domain consisting of a variable number of ~34 amino acid repeats. There appears to be a one-to-o ...
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