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Buffered Charcoal Yeast Extract Agar
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species ''Legionella pneumophila ''Legionella pneumophila'', the primary causative agent for Legionnaires' disease, Legionnaire's disease, is an Aerobic organism, aerobic, pleomorphic, Flagellum, flagellated, non-spore-forming, Gram-negative bacteria, Gram-negative bacterium. ' ...''. It has also been used for the laboratory diagnosis of ''Acanthamoeba'' keratitis, '' Francisella'' and '' Nocardia spp''. It contains L-cysteine amino acid and ferric pyrophosphate that assist in the growth of Legionnaire's species. The charcoal within the medium acts as a detoxicant because it decomposes hydrogen peroxide which is toxic to the legionellae. The yeast extract in BCYE is the rich source of nutrients (vitamins, nitrogen, and carbon) that the bacteria depends on for growth. BCYE also has ACES buffer which maintains an optimal pH le ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Agar
Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from " ogonori" and " tengusa". As found in nature, agar is a mixture of two components, the linear polysaccharide agarose and a heterogeneous mixture of smaller molecules called agaropectin. It forms the supporting structure in the cell walls of certain species of algae and is released on boiling. These algae are known as agarophytes, belonging to the Rhodophyta (red algae) phylum. The processing of food-grade agar removes the agaropectin, and the commercial product is essentially pure agarose. Agar has been used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture media for microbiological work. Agar can be used as a laxative; an appetite suppressant; a vegan substitute for gelatin; a thickener for soups; in fruit preserves, ice cream, and other desserts; as a clarifying ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
L-cysteine
Cysteine (; symbol Cys or C) is a semiessential proteinogenic amino acid with the formula . The thiol side chain in cysteine enables the formation of disulfide bonds, and often participates in enzymatic reactions as a nucleophile. Cysteine is chiral, but both D and L-cysteine are found in nature. LCysteine is a protein monomer in all biota, and D-cysteine acts as a signaling molecule in mammalian nervous systems. Cysteine is named after its discovery in urine, which comes from the urinary bladder or cyst, from Greek κύστις ''kýstis'', "bladder". The thiol is susceptible to oxidation to give the disulfide derivative cystine, which serves an important structural role in many proteins. In this case, the symbol Cyx is sometimes used. The deprotonated form can generally be described by the symbol Cym as well. When used as a food additive, cysteine has the E number E920. Cysteine is encoded by the codons UGU and UGC. Structure Like other amino acids (not as a residue of a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Potassium Hydroxide
Potassium hydroxide is an inorganic compound with the formula K OH, and is commonly called caustic potash. Along with sodium hydroxide (NaOH), KOH is a prototypical strong base. It has many industrial and niche applications, most of which utilize its caustic nature and its reactivity toward acids. An estimated 700,000 to 800,000 tonnes were produced in 2005. KOH is noteworthy as the precursor to most soft and liquid soaps, as well as numerous potassium-containing chemicals. It is a white solid that is dangerously corrosive. Properties and structure KOH exhibits high thermal stability. Because of this high stability and relatively low melting point, it is often melt-cast as pellets or rods, forms that have low surface area and convenient handling properties. These pellets become tacky in air because KOH is hygroscopic. Most commercial samples are ca. 90% pure, the remainder being water and carbonates. Its dissolution in water is strongly exothermic. Concentrated aqueous ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Legionella
''Legionella'' is a genus of gram-negative bacteria, gram-negative bacteria that can be seen using a silver stain or grown in a special media that contains cysteine, an amino acid. It is known to cause legionellosis (all illnesses caused by ''Legionella'') including a pneumonia-type illness called Legionnaires' disease and a mild flu-like illness called Pontiac fever. These bacteria are common in many places, like soil and water. There are over 50 species and 70 types (serotype, serogroups) identified. ''Legionella'' does not spread from person-to-person. Most individuals who are exposed to the bacteria do not get sick. Most outbreaks result from poorly maintained cooling towers. The cell wall of the ''Legionella'' bacteria has parts that determine its specific type. The structural arrangement and building blocks (sugars) in the cell wall help classify the bacteria. Etymology ''Legionella'' was named after 1976 Philadelphia Legionnaires' disease outbreak, a 1976 outbreak of a th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Activated Charcoal
"Activated" is a song by English singer Cher Lloyd. It was released on 22 July 2016 through Vixen Records. The song was made available to stream exclusively on ''Rolling Stone'' a day before to release (on 21 July 2016). Background In an interview with ''Rolling Stone'', Lloyd said about the song "I think when we first started writing 'Activated' – in the very beginning stages – I had said that I wanted a song eflectingexactly how I feel. t'sthis feeling that I have of just wanting to run as fast as I possibly can and just move. I wasn't going to just record something and release shit for the sake of having a song out, I wanted to feel something when I heard t and I wanted to feel like I did when I first started, like when I used to sing in my bedroom when I was 15." Critical reception Idolator's Robbie Daw said "Activated is a slinkly, sassy little R&B number, complete with the commanding line 'when I do the damn thing, just watch me.'" Estelle Tang of ''Elle'' said t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
ACES (buffer)
ACES (''N''-(2-acetamido)-2-aminoethanesulfonic acid) is a chemical compound that is one of Good's buffers. It was developed in the 1960s to provide buffer solutions with pH ranging from 6.15-8.35 for use in various applications. With a p''K''a of 6.9, it is often used as a buffering agent in biological and biochemical research. It is a zwitterionic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer. Applications ACES had been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. ACES use in isoelectric focusing of proteins has also been documented. Use of ACES has been published in a protocol for the analysis of bacterial autolysins in a discontinuous SDS-PAGE SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a Discontinuous electrophoresis, discontinuous electrophoretic system developed by Ulrich K. Laemml ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Yeast Extract
Yeast extracts consist of the cell contents of yeast without the cell walls; they are used as food additives or flavorings, or as nutrients for bacterial culture Growing media, media. They are often used to create savoury flavors and umami taste sensations and can be found in a large variety of packaged foods including frozen meals, crackers, snack foods, gravy, stock and more. They are rich in B vitamins (but not Vitamin B12, B12). Yeast extracts and Fermentation in food processing, fermented foods contain glutamic acid (free glutamates), an amino acid which adds an umami flavor. Glutamic acid is found in meat, cheese, fungi (mushrooms and yeast) and vegetables—such as broccoli and tomatoes. A number of other substances found in yeast extract provide aromas, some meat-like, when allowed to react under heat. The heat-autolytic process to make yeast extract of the autolysate type was invented in the 19th century by Justus von Liebig. Yeast cells are heated until they rupture, t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Liter
The litre ( Commonwealth spelling) or liter ( American spelling) (SI symbols L and l, other symbol used: ℓ) is a metric unit of volume. It is equal to 1 cubic decimetre (dm3), 1000 cubic centimetres (cm3) or 0.001 cubic metres (m3). A cubic decimetre (or litre) occupies a volume of (see figure) and is thus equal to one-thousandth of a cubic metre. The original French metric system used the litre as a base unit. The word ''litre'' is derived from an older French unit, the '' litron'', whose name came from Byzantine Greek—where it was a unit of weight, not volume—via Late Medieval Latin, and which equalled approximately 0.831 litres. The litre was also used in several subsequent versions of the metric system and is accepted for use with the SI, despite it not being an SI unit. The SI unit of volume is the cubic metre (m3). The spelling used by the International Bureau of Weights and Measures is "litre", [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Growth Medium
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different types of media are used for growing different types of cells. The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and those used for microbiological culture, which are used for growing microorganisms such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells. Types The most common growth media ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram
The gram (originally gramme; SI unit symbol g) is a Physical unit, unit of mass in the International System of Units (SI) equal to one thousandth of a kilogram. Originally defined in 1795 as "the absolute Mass versus weight, weight of a volume of pure water equal to Cube (algebra), the cube of the hundredth part of a metre [1 Cubic centimetre, cm3], and at Melting point of water, the temperature of Melting point, melting ice", the defining temperature (0 °C) was later changed to the temperature of maximum density of water (approximately 4 °C). Subsequent redefinitions agree with this original definition to within 30 Parts-per notation, parts per million (0.003%), with the maximum density of water remaining very close to 1 g/cm3, as shown by modern measurements. By the late 19th century, there was an effort to make the Base unit (measurement), base unit the kilogram and the gram a derived unit. In 1960, the new International System of Units defined a '' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
