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Back-crossing
Backcrossing is a crossing of a Hybrid (biology), hybrid with one of its parents or an individual genetically similar to its parent, to achieve offspring with a genetic identity closer to that of the parent. It is used in horticulture, animal breeding, and production of gene knockout organisms. Backcrossed hybrids are sometimes described with acronym "BC"; for example, an F1 hybrid crossed with one of its parents (or a genetically similar individual) can be termed a BC1 hybrid, and a further cross of the BC1 hybrid to the same parent (or a genetically similar individual) produces a BC2 hybrid. Plants Advantages * If the recurrent parent is an elite genotype, at the end of the backcrossing programme, an elite genotype is recovered. * As no "new" Genetic recombination, recombination results, the elite combination is not lost. Disadvantages * It works poorly for quantitative traits. * It is more restricted for recessive traits. * In practice, sections of genome from the nonrecurre ...
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Hybrid (biology)
In biology, a hybrid is the offspring resulting from combining the qualities of two organisms of different varieties, subspecies, species or genera through sexual reproduction. Generally, it means that each cell has genetic material from two different organisms, whereas an individual where some cells are derived from a different organism is called a chimera. Hybrids are not always intermediates between their parents such as in blending inheritance (a now discredited theory in modern genetics by particulate inheritance), but can show hybrid vigor, sometimes growing larger or taller than either parent. The concept of a hybrid is interpreted differently in animal and plant breeding, where there is interest in the individual parentage. In genetics, attention is focused on the numbers of chromosomes. In taxonomy, a key question is how closely related the parent species are. Species are reproductively isolated by strong barriers to hybridization, which include genetic and morph ...
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Wild Type
The wild type (WT) is the phenotype of the typical form of a species as it occurs in nature. Originally, the wild type was conceptualized as a product of the standard "normal" allele at a locus, in contrast to that produced by a non-standard, " mutant" allele. "Mutant" alleles can vary to a great extent, and even become the wild type if a genetic shift occurs within the population. Continued advancements in genetic mapping technologies have created a better understanding of how mutations occur and interact with other genes to alter phenotype. It is now regarded that most or all gene loci exist in a variety of allelic forms, which vary in frequency throughout the geographic range of a species, and that a uniform wild type does not exist. In general, however, the most prevalent allele – i.e., the one with the highest gene frequency – is the one deemed wild type. The concept of wild type is useful in some experimental organisms such as fruit flies ''Drosophila melanogaster'', ...
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Horticulture
Horticulture (from ) is the art and science of growing fruits, vegetables, flowers, trees, shrubs and ornamental plants. Horticulture is commonly associated with the more professional and technical aspects of plant cultivation on a smaller and more controlled scale than agronomy. There are various divisions of horticulture because plants are grown for a variety of purposes. These divisions include, but are not limited to: propagation, arboriculture, landscaping, floriculture and turf maintenance. For each of these, there are various professions, aspects, tools used and associated challenges -- each requiring highly specialized skills and knowledge on the part of the horticulturist. Typically, horticulture is characterized as the ornamental, small-scale and non-industrial cultivation of plants; horticulture is distinct from gardening by its emphasis on scientific methods, plant breeding, and technical cultivation practices, while gardening, even at a professional level, tends ...
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Plant Reproduction
Plants may reproduce sexually or asexually. Sexual reproduction produces offspring by the fusion of gametes, resulting in offspring genetically different from either parent. Vegetative reproduction produces new individuals without the fusion of gametes, resulting in clonal plants that are genetically identical to the parent plant and each other, unless mutations occur. In asexual reproduction, only one parent is involved. Asexual reproduction Asexual reproduction does not involve the production and fusion of male and female gametes. Asexual reproduction may occur through budding, fragmentation, spore formation, regeneration and vegetative propagation. Asexual reproduction is a type of reproduction where the offspring comes from one parent only, thus inheriting the characteristics of the parent. Asexual reproduction in plants occurs in two fundamental forms, vegetative reproduction and agamospermy. Vegetative reproduction involves a vegetative piece of the original pla ...
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Incest
Incest ( ) is sexual intercourse, sex between kinship, close relatives, for example a brother, sister, or parent. This typically includes sexual activity between people in consanguinity (blood relations), and sometimes those related by lineage (anthropology), lineage. It is condemned and considered immoral in many societies. It can lead to an increased risk of genetic disorders in children in case of pregnancy from incestuous sex. The incest taboo is one of the most widespread of all cultural taboos, both in present and in past societies. Most modern societies have laws regarding incest or social restrictions on closely consanguineous marriages. In societies where it is illegal, consensual adult incest is seen by some as a victimless crime. Some cultures extend the incest taboo to relatives with no consanguinity, such as Milk kinship, milk-siblings, stepsiblings, and adoptive siblings, albeit sometimes with less intensity. Third-degree relatives (such as half-aunt, half-nephew ...
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Introgression
Introgression, also known as introgressive hybridization, in genetics is the transfer of genetic material from one species into the gene pool of another by the repeated backcrossing of an interspecific hybrid with one of its parent species. Introgression is a long-term process, even when artificial; it may take many hybrid generations before significant backcrossing occurs. This process is distinct from most forms of gene flow in that it occurs between two populations of different species, rather than two populations of the same species. Introgression also differs from simple Hybridization (biology), hybridization. Simple hybridization results in a relatively even mixture; gene and allele frequencies in the first generation will be a uniform mix of two parental species, such as that observed in mules. Introgression, on the other hand, results in a complex, highly variable mixture of genes, and may only involve a minimal percentage of the donor genome. Definition Introgression or i ...
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Expected Value
In probability theory, the expected value (also called expectation, expectancy, expectation operator, mathematical expectation, mean, expectation value, or first Moment (mathematics), moment) is a generalization of the weighted average. Informally, the expected value is the arithmetic mean, mean of the possible values a random variable can take, weighted by the probability of those outcomes. Since it is obtained through arithmetic, the expected value sometimes may not even be included in the sample data set; it is not the value you would expect to get in reality. The expected value of a random variable with a finite number of outcomes is a weighted average of all possible outcomes. In the case of a continuum of possible outcomes, the expectation is defined by Integral, integration. In the axiomatic foundation for probability provided by measure theory, the expectation is given by Lebesgue integration. The expected value of a random variable is often denoted by , , or , with a ...
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Positive Selectable Marker
Positive is a property of positivity and may refer to: Mathematics and science * Positive formula, a logical formula not containing negation * Positive number, a number that is greater than 0 * Plus sign, the sign "+" used to indicate a positive number * Positive operator, a type of linear operator in mathematics * Positive result, a result that has been found significant in statistical hypothesis testing * Positive test, a diagnostic test result that indicates some parameter being evaluated was present * Positive charge, one of the two types of electrical charge * Positive (electrical polarity), in electrical circuits * Positive lens, in optics * Positive (photography), a positive image, in which the color and luminance correlates directly with that in the depicted scene * Positive sense, said of an RNA sequence that codes for a protein Philosophy and humanities * Affirmative (policy debate), the team which affirms the resolution * Negative and positive rights, conce ...
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Stem Cell
In multicellular organisms, stem cells are undifferentiated or partially differentiated cells that can change into various types of cells and proliferate indefinitely to produce more of the same stem cell. They are the earliest type of cell in a cell lineage. They are found in both embryonic and adult organisms, but they have slightly different properties in each. They are usually distinguished from progenitor cells, which cannot divide indefinitely, and precursor or blast cells, which are usually committed to differentiating into one cell type. In mammals, roughly 50 to 150 cells make up the inner cell mass during the blastocyst stage of embryonic development, around days 5–14. These have stem-cell capability. '' In vivo'', they eventually differentiate into all of the body's cell types (making them pluripotent). This process starts with the differentiation into the three germ layers – the ectoderm, mesoderm and endoderm – at the gastrulation stage. However, whe ...
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Cell Culture
Cell culture or tissue culture is the process by which cell (biology), cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been Cell isolation, isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or rich growth medium, medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (Carbon dioxide, CO2, Oxygen, O2), and regulates the physio-chemical environment (Buffer solution, pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. T ...
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Introgression Line
Introgression, also known as introgressive hybridization, in genetics is the transfer of genetic material from one species into the gene pool of another by the repeated backcrossing of an interspecific hybrid with one of its parent species. Introgression is a long-term process, even when artificial; it may take many hybrid generations before significant backcrossing occurs. This process is distinct from most forms of gene flow in that it occurs between two populations of different species, rather than two populations of the same species. Introgression also differs from simple hybridization. Simple hybridization results in a relatively even mixture; gene and allele frequencies in the first generation will be a uniform mix of two parental species, such as that observed in mules. Introgression, on the other hand, results in a complex, highly variable mixture of genes, and may only involve a minimal percentage of the donor genome. Definition Introgression or introgressive hybridizat ...
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