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Analytical Band Centrifugation
Analytical band centrifugation (ABC) (also known as analytical band ultracentrifugation, or band sedimentation-velocity), is a specialized ultracentrifugation procedure, where unlike the typical use of (boundary) sedimentation velocity analytical ultracentrifugation (SV-AUC) wherein a homogenous bulk solution is centrifuged, in ABC a thin (~15 μL, ~500 μm) sample is layered on top of a bulk solvent and then centrifuged. The method is distinguished from ''zone-sedimentation'' in that a stabilizing density gradient is self-generated during centrifugation, through the use of a higher density (than the sample) bulk "binary solvent", containing both a solvent (i.e. H2O), and a second component (small molecules, i.e. CsCl) that will sediment to form a stabilizing density gradient for the sample. ABC also requires specially designed analytical ultracentrifuge cells, as the sample is not manually applied by pipette but instead automatically delivered via capillary under low g-forces ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Band Vs Boundary Ultracentrifugation
Band or BAND may refer to: Places *Bánd, a village in Hungary * Band, Iran, a village in Urmia County, West Azerbaijan Province, Iran *Band, Mureș, a commune in Romania * Band-e Majid Khan, a village in Bukan County, West Azerbaijan Province, Iran People *Band (surname), various people with the surname Arts, entertainment, and media Music *Musical ensemble, a group of people who perform instrumental or vocal music **Band (rock and pop), a small ensemble that plays rock or pop **Concert band, an ensemble of woodwind, brass, and percussion instruments **Dansband, band playing popular music for a partner-dancing audience **Jazz band, a musical ensemble that plays jazz music **Marching band, a group of instrumental musicians who generally perform outdoors **School band, a group of student musicians who rehearse and perform instrumental music *The Band, a Canadian-American rock and roll group ** ''The Band'' (album), The Band's eponymous 1969 album * "Bands" (song), by American rap ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Caesium Chloride
Caesium chloride or cesium chloride is the inorganic compound with the formula Caesium, CsChloride, Cl. This colorless salt is an important source of caesium ions in a variety of niche applications. Its crystal structure forms a major structural type where each caesium ion is coordinated by 8 chloride ions. Caesium chloride dissolves in water. CsCl changes to NaCl structure on heating. Caesium chloride occurs naturally as impurities in carnallite (up to 0.002%), sylvite and kainite. Less than 20 tonnes of CsCl is produced annually worldwide, mostly from a caesium-bearing mineral pollucite. Caesium chloride is widely used in isopycnic centrifugation for separating various types of DNA. It is a reagent in analytical chemistry, where it is used to identify ions by the color and morphology of the precipitate. When enriched in radionuclide, radioisotopes, such as 137CsCl or 131CsCl, caesium chloride is used in nuclear medicine applications such as treatment of cancer and diagnosis of m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Adeno-associated Virus
Adeno-associated viruses (AAV) are small viruses that infect humans and some other primate species. They belong to the genus '' Dependoparvovirus'', which in turn belongs to the family ''Parvoviridae''. They are small (approximately 26 nm in diameter) replication-defective, nonenveloped viruses and have linear single-stranded DNA (ssDNA) genome of approximately 4.8 kilobases (kb). Several features make AAV an attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell. In the native virus, however, integration of virally carried genes into the host genome does occur. Integration can be important for certain applications, but can also have unwanted consequences. Recent human clinical trials using AAV for gene therapy in the retina have shown ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Svedberg
In chemistry, a Svedberg unit or svedberg (symbol S, sometimes Sv) is a non- SI metric unit for sedimentation coefficients. The Svedberg unit offers a measure of a particle's size indirectly based on its sedimentation rate under acceleration (i.e. how fast a particle of given size and shape settles out of suspension). The svedberg is a measure of time, defined as exactly 10−13 seconds (100 fs). For biological macromolecules like ribosomes, the sedimentation rate is typically measured as the rate of travel in a centrifuge tube subjected to high g-force. The svedberg (S) is distinct from the SI unit sievert or the non-SI unit sverdrup, which also use the symbol Sv, and to the SI unit Siemens which uses the symbol S too. Naming The unit is named after the Swedish chemist Theodor Svedberg (1884–1971), winner of the 1926 Nobel Prize in chemistry for his work on disperse systems, colloids and his invention of the ultracentrifuge. Factors The Svedberg coef ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sedimentation
Sedimentation is the deposition of sediments. It takes place when particles in suspension settle out of the fluid in which they are entrained and come to rest against a barrier. This is due to their motion through the fluid in response to the forces acting on them: these forces can be due to gravity, centrifugal acceleration, or electromagnetism. Settling is the falling of suspended particles through the liquid, whereas sedimentation is the final result of the settling process. In geology, sedimentation is the deposition of sediments which results in the formation of sedimentary rock. The term is broadly applied to the entire range of processes that result in the formation of sedimentary rock, from initial erosion through sediment transport and settling to the lithification of the sediments. However, the strict geological definition of sedimentation is the mechanical deposition of sediment particles from an initial suspension in air or water. Sedimentation may pertain to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Centrifugation
Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. The denser components of the mixture migrate away from the axis of the centrifuge, while the less dense components of the mixture migrate towards the axis. Chemists and biologists may increase the effective gravitational force of the test tube so that the precipitate (pellet) will travel quickly and fully to the bottom of the tube. The remaining liquid that lies above the precipitate is called a supernatant or supernate. There is a correlation between the size and density of a particle and the rate that the particle separates from a heterogeneous mixture, when the only force applied is that of gravity. The larger the size and the larger the density of the particles, the faster they separate from the mixture. By applying a larger effective gravitational force to the mixture, like ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Differential Centrifugation
In biochemistry and cell biology, differential centrifugation (also known as differential velocity centrifugation) is a common procedure used to separate organelles and other sub-cellular particles based on their sedimentation rate. Although often applied in biological analysis, differential centrifugation is a general technique also suitable for crude purification of non-living suspended particles (e.g. nanoparticles, colloidal particles, viruses). In a typical case where differential centrifugation is used to analyze cell-biological phenomena (e.g. organelle distribution), a tissue sample is first lysed to break the cell membranes and release the organelles and cytosol. The lysate is then subjected to repeated centrifugations, where particles that sediment sufficiently quickly at a given centrifugal force for a given time form a compact "pellet" at the bottom of the centrifugation tube. After each centrifugation, the ''supernatant'' (non-pelleted solution) is removed from t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laboratory Techniques
A laboratory (; ; colloquially lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratories are found in a variety of settings such as schools, universities, privately owned research institutions, corporate research and testing facilities, government regulatory and forensic investigation centers, physicians' offices, clinics, hospitals, regional and national referral centers, and even occasionally personal residences. Overview The organisation and contents of laboratories are determined by the differing requirements of the specialists working within. A physics laboratory might contain a particle accelerator or vacuum chamber, while a metallurgy laboratory could have apparatus for casting or refining metals or for testing their strength. A chemist or biologist might use a wet laboratory, while a psychologist's laboratory might be a room with one-way mirrors and hidden cameras in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
