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Agroinfiltration
Agroinfiltration is a method used in plant biology and especially lately in plant biotechnology to induce transient expression of genes in a plant, or isolated leaves from a plant, or even in cultures of plant cells, in order to produce a desired protein. In the method, a suspension of ''Agrobacterium tumefaciens'' is introduced into a plant leaf by direct injection or by vacuum infiltration, or brought into association with plant cells immobilised on a porous support (plant cell packs), whereafter the bacteria transfer the desired gene into the plant cells via transfer of T-DNA. The main benefit of agroinfiltration when compared to the more traditional plant transformation is speed and convenience, although yields of the recombinant protein are generally also higher and more consistent. The first step is to introduce a gene of interest to a strain of ''Agrobacterium tumefaciens''. Subsequently, the strain is grown in a liquid culture and the resulting bacteria are washed and ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Transient Expression
Transient expression, more frequently referred to "transient gene expression", is the temporary expression of genes that are expressed for a short time after nucleic acid, most frequently plasmid DNA encoding an expression cassette, has been introduced into eukaryotic cells with a chemical delivery agent like calcium phosphate (CaPi) or polyethyleneimine (PEI). However, unlike "stable expression," the foreign DNA does not fuse with the host cell DNA, resulting in the inevitable loss of the vector after several cell replication cycles. The majority of transient gene expressions are done with cultivated animal cells. The technique is also used in plant cells; however, the transfer of nucleic acids into these cells requires different methods than those with animal cells. In both plants and animals, transient expression should result in a time-limited use of transferred nucleic acids, since any long-term expression would be called "stable expression." Methodology varies depending on ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Agrobacterium Tumefaciens
''Agrobacterium tumefaciens'' (also known as ''Rhizobium radiobacter'') is the causal agent of crown gall disease (the formation of tumours) in over 140 species of eudicots. It is a rod-shaped, Gram-negative soil bacterium. Symptoms are caused by the insertion of a small segment of DNA (known as T-DNA, for 'transfer DNA', not to be confused with tRNA that transfers amino acids during protein synthesis), from a plasmid into the plant cell, which is incorporated at a semi-random location into the plant genome. Plant genomes can be engineered by use of '' Agrobacterium'' for the delivery of sequences hosted in T-DNA binary vectors. ''Agrobacterium tumefaciens'' is an Alphaproteobacterium of the family Rhizobiaceae, which includes the nitrogen-fixing legume symbionts. Unlike the nitrogen-fixing symbionts, tumor-producing ''Agrobacterium'' species are pathogenic and do not benefit the plant. The wide variety of plants affected by ''Agrobacterium'' makes it of great concern to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nicotiana Benthamiana
''Nicotiana benthamiana'', colloquially known as benth or benthi, is a species of ''Nicotiana'' indigenous to Australia. It is a close relative of tobacco. A synonym for this species is ''Nicotiana suaveolens'' var. ''cordifolia'', a description given by George Bentham in ''Flora Australiensis'' in 1868. This was transferred to ''Nicotiana benthamiana'' by Karel Domin in ''Bibliotheca Botanica'' (1929), honoring the original author in the specific epithet. History The plant was used by people of Australia as a stimulant, containing nicotine and other alkaloids, before the introduction of commercial tobacco ('' N. tabacum'' and '' N. rustica''). Indigenous names for it include ''tjuntiwari'' and ''muntju''. It was first collected on the north coast of Australia by Benjamin Bynoe on a voyage of in 1837. Description The herbaceous plant is found amongst rocks on hills and cliffs throughout the northern regions of Australia. Variable in height and habit, the species may be ere ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Leaf
A leaf (: leaves) is a principal appendage of the plant stem, stem of a vascular plant, usually borne laterally above ground and specialized for photosynthesis. Leaves are collectively called foliage, as in "autumn foliage", while the leaves, stem, flower, and fruit collectively form the Shoot (botany), shoot system. In most leaves, the primary Photosynthesis, photosynthetic Tissue (biology), tissue is the palisade mesophyll and is located on the upper side of the blade or lamina of the leaf, but in some species, including the mature foliage of ''Eucalyptus'', palisade mesophyll is present on both sides and the leaves are said to be isobilateral. The leaf is an integral part of the stem system, and most leaves are flattened and have distinct upper (Glossary of botanical terms#adaxial, adaxial) and lower (Glossary of botanical terms#abaxial, abaxial) surfaces that differ in color, Trichome, hairiness, the number of stomata (pores that intake and output gases), the amount and ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
RNA Interference
RNA interference (RNAi) is a biological process in which RNA molecules are involved in sequence-specific suppression of gene expression by double-stranded RNA, through translational or transcriptional repression. Historically, RNAi was known by other names, including ''co-suppression'', ''post-transcriptional gene silencing'' (PTGS), and ''quelling''. The detailed study of each of these seemingly different processes elucidated that the identity of these phenomena were all actually RNAi. Andrew Fire and Craig Mello shared the 2006 Nobel Prize in Physiology or Medicine for their work on RNAi in the nematode worm ''Caenorhabditis elegans'', which they published in 1998. Since the discovery of RNAi and its regulatory potentials, it has become evident that RNAi has immense potential in suppression of desired genes. RNAi is now known as precise, efficient, stable and better than antisense therapy for gene suppression. Antisense RNA produced intracellularly by an expression vector may be ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Acetosyringone
Acetosyringone is a phenolic natural product and a chemical compound related to acetophenone and 2,6-dimethoxyphenol. It was first described in relation to lignan/phenylpropanoid-type phytochemicals, with isolation from a variety of plant sources, in particular, in relation to wounding and other physiologic changes. Occurrence and biological role Historically, this substance has been best known for its involvement in plant-pathogen recognition, especially its role as a signal attracting and transforming unique, oncogenic bacteria in genus ''Agrobacterium''. The ''virA'' gene on the Ti plasmid of ''Agrobacterium tumefaciens'' and the Ri plasmid of '' Agrobacterium rhizogenes'' is used by these soil bacteria to infect plants, via its encoding for a receptor for acetosyringone and other phenolic phytochemicals exuded by plant wounds. This compound also allows higher transformation efficiency in plants, as shown in ''A. tumefaciens''-mediated transformation procedures, and so is ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bunyaviridae
''Bunyaviricetes'' is a class of segmented negative-strand RNA viruses with mainly tripartite genomes. Member viruses infect arthropods, plants, protozoans, and vertebrates. The name ''Bunyaviricetes'' derives from Bunyamwera, where the virus Bunyamwera virus was first discovered. Bunyaviruses belong to the fifth group of the Baltimore classification system, which includes viruses with a negative-sense, single-stranded RNA genome. They have an enveloped, spherical virion. Though generally found in arthropods or rodents, certain viruses in this class occasionally infect humans. Some of them also infect plants. In addition, there is a group of bunyaviruses whose replication is restricted to arthropods and is known as insect-specific bunyaviruses. A majority of bunyaviruses are vector-borne. With the exception of Hantaviruses and Arenaviruses, all viruses in the ''Bunyaviricetes'' class are transmitted by arthropods (mosquitos, tick, or sandfly). Hantaviruses are transmitted ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tospovirus
''Orthotospovirus'' is a genus of negative-strand RNA viruses, in the family ''Tospoviridae'' of the order '' Elliovirales,'' which infects plants. Tospoviruses take their name from the species Tomato spotted wilt virus (TSWV) which was discovered in Australia in 1919. TSWV remained the only known member of the family until the early 1990s when genetic characterisation of plant viruses became more common. There are now 28 species in the genus with more being discovered on a regular basis. Member viruses infect over eight hundred plant species from 82 different families. Genome Tospoviruses have a negative-sense, single-strand RNA genome. The genome resembles that of the genus '' Phlebovirus''. It is linear and is 17.2 kb in size. It is divided into three segments termed S (2.9kb), M (5.4kb), and L (8.9kb). The M and S RNA segments encode for proteins in an ambisense direction. Transmission Tospoviruses are arboviruses usually vectored by thrips. At least ten species of t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tomato
The tomato (, ), ''Solanum lycopersicum'', is a plant whose fruit is an edible Berry (botany), berry that is eaten as a vegetable. The tomato is a member of the nightshade family that includes tobacco, potato, and chili peppers. It originated from and was domesticated in western South America. It was introduced to the Old World by the Spanish in the Columbian exchange in the 16th century. Tomato plants are vines, largely Annual plant, annual and vulnerable to frost, though sometimes living longer in greenhouses. The flowers are able to self-fertilise. Modern varieties have been bred to ripen uniformly red, in a process that has impaired the fruit's sweetness and flavor. There are thousands of cultivars, varying in size, color, shape, and flavor. Tomatoes are attacked by many insect pests and nematodes, and are subject to diseases caused by viruses and by mildew and blight fungi. The tomato has a strong savoury umami flavor, and is an important ingredient in cuisines around ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tomato Spotted Wilt Virus
Tomato spotted wilt virus (TSWV) is a spherical Sense (molecular biology), negative-sense RNA virus. Transmitted by thrips, it causes serious losses in economically important crops and it is one of the most economically devastating plant viruses in the world. Transmission and lifespan The circulative propagative transmission of TSWV is carried out by at least 10 different species of thrips. The most common species is ''Frankliniella occidentalis'' (western flower thrips) as it is the Vector (epidemiology), vector that predominantly transmits TSWV globally and in greenhouses. The rapid developmental and reproductive rate of the thrips contributes to the spread of TSWV. The time needed for insects to acquire the virus (acquisition period) and the time needed for the virus to move from the insect to the plant (inoculation) for TSWV varies depends on the vector species. For ''F. occidentalis'', the acquisition and inoculation of TSWV can be as short as 5 minutes. However, the acq ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
