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Suspension Culture
A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. Suspension culture is one of the two classical types of cell culture, the other being adherent culture. The history of suspension cell culture closely aligns with the history of cell culture overall, but differs in maintenance methods and commercial applications. The cells themselves can either be derived from homogenized tissue or from heterogenous cell solutions. Suspension cell culture is commonly used to culture nonadhesive cell lines like hematopoietic cells, plant cell Plant cells are the cells present in Viridiplantae, green plants, photosynthetic eukaryotes of the kingdom Plantae. Their distinctive features include primary cell walls containing cellulose, hemicelluloses and pectin, the presence of plastids ...s, and Insect cell culture, insect cells. While ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cells
Cell most often refers to: * Cell (biology), the functional basic unit of life * Cellphone, a phone connected to a cellular network * Clandestine cell, a penetration-resistant form of a secret or outlawed organization * Electrochemical cell, a device used to convert chemical energy to electrical energy * Prison cell, a room used to hold people in prisons Cell may also refer to: Arts, entertainment, and media Fictional entities * Cell (comics), a Marvel comic book character * Cell (''Dragon Ball''), a character in the manga series ''Dragon Ball'' Literature * ''Cell'' (novel), a 2006 horror novel by Stephen King * "Cells", poem, about a hungover soldier in gaol, by Rudyard Kipling * ''The Cell'' (play), an Australian play by Robert Wales Music * Cell (music), a small rhythmic and melodic design that can be isolated, or can make up one part of a thematic context * Cell (American band) * Cell (Japanese band) * ''Cell'' (album), a 2004 album by Plastic Tree * ''Cells'', a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Leonard Hayflick
Leonard Hayflick (May 20, 1928 – August 1, 2024) was an American anatomist who was Professor of Anatomy at the UCSF School of Medicine, and was Professor of Medical Microbiology at Stanford University School of Medicine. He was also past president of the Gerontological Society of America and was a founding member of the council of the National Institute on Aging (NIA). The recipient of a number of research prizes and awards, including the 1991 Sandoz Prize for Gerontological Research, he studied the senescence, ageing process for more than fifty years. He is known for discovering that normal human Cell (biology), cells divide for a limited number of times ''in vitro'' (refuting the contention by Alexis Carrel that normal body cells are biological immortality, immortal). This is known as the Hayflick limit. His discoveries overturned a 60-year old dogma that all cultured cells are immortal. Hayflick demonstrated that normal cells have a memory and can remember what doubling le ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chinese Hamster Ovary Cell
Chinese hamster ovary (CHO) cells are a family of immortalized cell lines derived from epithelial cells of the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, and particularly since the 1980s to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. History Chinese hamsters were first used in medical research in 1919, when Dr. E.T. Hsieh of the Peking Union Medical College used captured hamsters from the local fields for typing pneumococci. They were subsequently found by Jocelyn Smyly and Charles Young, two other doctors at the same institute, to be excellent vectors for transmission of kala-azar ( visceral leishmaniasis), facilitating ''Leishmania'' research. Unsuccessful attempts were m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Morphology (biology)
Morphology (from Ancient Greek μορφή (morphḗ) "form", and λόγος (lógos) "word, study, research") is the study of the form and structure of organisms and their specific structural features. This includes aspects of the outward appearance (shape, structure, color, pattern, size), as well as the form and structure of internal parts like bones and organs, i.e., anatomy. This is in contrast to physiology, which deals primarily with function. Morphology is a branch of life science dealing with the study of the overall structure of an organism or taxon and its component parts. History The etymology of the word "morphology" is from the Ancient Greek (), meaning "form", and (), meaning "word, study, research". While the concept of form in biology, opposed to function, dates back to Aristotle (see Aristotle's biology), the field of morphology was developed by Johann Wolfgang von Goethe (1790) and independently by the German anatomist and physiologist Karl Fried ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Senescence
Senescence () or biological aging is the gradual deterioration of Function (biology), functional characteristics in living organisms. Whole organism senescence involves an increase in mortality rate, death rates or a decrease in fecundity with increasing age, at least in the later part of an organism's biological life cycle, life cycle. However, the resulting effects of senescence can be delayed. The 1934 discovery that calorie restriction can Life extension, extend lifespans by 50% in rats, the existence of species having negligible senescence, and the existence of potentially immortal organisms such as members of the genus ''Hydra (genus), Hydra'' have motivated research into Life extension, delaying senescence and thus age-related diseases. Rare human mutations can cause accelerated aging diseases. Environmental Gerontogens, factors may affect aging – for example, overexposure to ultraviolet radiation accelerates skin aging. Different parts of the body may age at different ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hemocytometer
The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber. This chamber is engraved with a laser-etched grid of perpendicular lines. The device is carefully crafted so that the area bounded by the lines is known, and the depth of the chamber is also known. By observing a defined area of the grid, it is therefore possible to count the number of cells or particles in a specific volume of fluid, and thereby calculate the concentration of cells in the fluid overall. A well used type of hemocytometer is the ''Neubauer'' counting chamber. Other types of hemocytometers with different rulings are in use for different applications. Fuchs-Rosenthal rulings, commonly used for spinal fluid counting, Howard Mold rulings us ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
201107 Shaker
Eleven or 11 may refer to: *11 (number) * One of the years 11 BC, AD 11, 1911, 2011 Literature * ''Eleven'' (novel), a 2006 novel by British author David Llewellyn *''Eleven'', a 1970 collection of short stories by Patricia Highsmith *''Eleven'', a 2004 children's novel in The Winnie Years by Lauren Myracle *''Eleven'', a 2008 children's novel by Patricia Reilly Giff *''Eleven'', a short story by Sandra Cisneros Music * Eleven (band), an American rock band * Eleven: A Music Company, an Australian record label *Up to eleven, an idiom from popular culture, coined in the movie ''This Is Spinal Tap'' Albums * ''11'' (The Smithereens album), 1989 * ''11'' (Ua album), 1996 * ''11'' (Bryan Adams album), 2008 * ''11'' (Sault album), 2022 * ''Eleven'' (Harry Connick, Jr. album), 1992 * ''Eleven'' (22-Pistepirkko album), 1998 * ''Eleven'' (Sugarcult album), 1999 * ''Eleven'' (B'z album), 2000 * ''Eleven'' (Reamonn album), 2010 * ''Eleven'' (Martina McBride album), 2011 * ''Eleven'' (Mr F ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Eagle's Minimal Essential Medium
Minimal essential medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in ''Science'' that can be used to maintain cells in tissue culture. It is based on six salts and glucose described in Earle's salts in 1943: calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, sodium phosphate and sodium bicarbonate. The medium is further supplemented with thirteen essential amino acids, and eight vitamins: thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3), pantothenic acid (vitamin B5), pyrodoxine (vitamin B6), folic acid (vitamin B9), choline, and myo-inositol (originally known as vitamin B8). Many variations of this medium have been developed, mostly adding additional vitamins, amino acids, and/or other nutrients. Eagle developed his earlier "Basal Medium Eagle" (BME) in 1955–1957 on mouse L cells and human HeLa cells, with 13 essential amino acids and 9 vitamins added. BME contains biotin (vita ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Surfactant
Surfactants are chemical compounds that decrease the surface tension or interfacial tension between two liquids, a liquid and a gas, or a liquid and a solid. The word ''surfactant'' is a Blend word, blend of "surface-active agent", coined in 1950. As they consist of a water-repellent and a water-attracting part, they enable water and oil to mix; they can form foam and facilitate the detachment of dirt. Surfactants are among the most widespread and commercially important chemicals. Private households as well as many industries use them in large quantities as detergent, detergents and cleaning agents, but also for example as emulsion#Emulsifiers, emulsifiers, wetting agents, foaming agents, Antistatic agent, antistatic additives, or dispersants. Surfactants occur naturally in traditional plant-based detergents, e.g. Aesculus, horse chestnuts or Sapindus, soap nuts; they can also be found in the secretions of some caterpillars. Today one of the most commonly used anionic surfa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Shear Force
In solid mechanics, shearing forces are unaligned forces acting on one part of a Rigid body, body in a specific direction, and another part of the body in the opposite direction. When the forces are Collinearity, collinear (aligned with each other), they are called ''tension forces'' or ''compression forces''. Shear force can also be defined in terms of Plane (geometry), planes: "If a plane is passed through a body, a force acting along this plane is called a ''shear force'' or ''shearing force''." Force required to shear steel This section calculates the force required to cut a piece of material with a shearing action. The relevant information is the area of the material being sheared, i.e. the area across which the shearing action takes place, and the shear strength of the material. A round bar of steel is used as an example. The shear strength is calculated from the tensile strength using a factor which relates the two strengths. In this case 0.6 applies to the example steel, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
White Blood Cell
White blood cells (scientific name leukocytes), also called immune cells or immunocytes, are cells of the immune system that are involved in protecting the body against both infectious disease and foreign entities. White blood cells are generally larger than red blood cells. They include three main subtypes: granulocytes, lymphocytes and monocytes. All white blood cells are produced and derived from multipotent cells in the bone marrow known as hematopoietic stem cells. Leukocytes are found throughout the body, including the blood and lymphatic system. All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) and platelets. The different white blood cells are usually classified by cell lineage ( myeloid cells or lymphoid cells). White blood cells are part of the body's immune system. They help the body fight infection and other diseases. Types of white blood cells are granulocytes (neutrophils, eosino ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
