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Southern Blotting
Southern blot is a method used for detection and quantification of a specific DNA sequence in DNA samples. This method is used in molecular biology. Briefly, purified DNA from a biological sample (such as blood or tissue) is digested with restriction enzymes, and the resulting DNA fragments are separated by electrophoresis using an electric current to move them through a sieve-like gel or matrix, which allows smaller fragments to move faster than larger fragments. The DNA fragments are transferred out of the gel or matrix onto a solid membrane, which is then exposed to a DNA probe labeled with a radioactive, fluorescent, or chemical tag. The tag allows any DNA fragments containing complementary sequences with the DNA probe sequence to be visualized within the Southern blot. The Southern blotting combines the transfer of electrophoresis-separated DNA fragments to a filter membrane in a process called blotting, and the subsequent fragment detection by probe hybridization. T ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Recombinant DNA- Southern Blot (1)
Recombinant may refer to: * Recombinant organism – an organism that contains a different combination of alleles from either of its parents. * Recombinant DNA – a form of artificial DNA sequence * Recombinant protein - artificially produced (and often purified) protein * Recombinant virus – a virus formed by recombining genetic material * VRLA – a valve regulated lead acid (VRLA) battery that is also referred to as a recombinant battery *''InSoc Recombinant'' – an album by synthpop band Information Society See also *Recombination (other) {{disambiguation ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Eastern Blotting
The eastern blot, or eastern blotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids, phosphates, and glycoconjugates. It is most often used to detect carbohydrate epitopes. Thus, eastern blot can be considered an extension of the biochemical technique of western blot. Multiple techniques have been described by the term "eastern blot(ting)", most use phosphoprotein blotted from sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel on to a polyvinylidene fluoride or nitrocellulose membrane. Transferred proteins are analyzed for post-translational modifications using probes that may detect lipids, carbohydrate, phosphorylation or any other protein modification. Eastern blotting should be used to refer to methods that detect their targets through specific interaction of the post-translational modifications and the probe, distinguishing them from a standard far-western blot. In principle, easter ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gel Electrophoresis
Gel electrophoresis is an electrophoresis method for separation and analysis of biomacromolecules (DNA, RNA, proteins, etc.) and their fragments, based on their size and charge through a gel. It is used in clinical chemistry to separate proteins by charge or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments, or to separate proteins by charge. Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a gel matrix of agarose, polyacrylamide, or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving. Proteins are separated by the charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Endonuclease
In molecular biology, endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain (namely DNA or RNA). Some, such as deoxyribonuclease I, cut DNA relatively nonspecifically (with regard to sequence), while many, typically called '' restriction endonucleases'' or ''restriction enzymes'', cleave only at very specific nucleotide sequences. Endonucleases differ from exonucleases, which cleave the ends of recognition sequences instead of the middle (''endo'') portion. Some enzymes known as "exo-endonucleases", however, are not limited to either nuclease function, displaying qualities that are both endo- and exo-like. Evidence suggests that endonuclease activity experiences a lag compared to exonuclease activity. Restriction enzymes are endonucleases from eubacteria and archaea that recognize a specific DNA sequence. The nucleotide sequence recognized for cleavage by a restriction enzyme is called the ''restriction site''. Typically, a restriction ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Frederick Sanger
Frederick Sanger (; 13 August 1918 – 19 November 2013) was a British biochemist who received the Nobel Prize in Chemistry twice. He won the 1958 Chemistry Prize for determining the amino acid sequence of insulin and numerous other proteins, demonstrating in the process that each had a unique, definite structure; this was a foundational discovery for the central dogma of molecular biology. At the newly constructed Laboratory of Molecular Biology in Cambridge, he developed and subsequently refined the first-ever DNA sequencing technique, which vastly expanded the number of feasible experiments in molecular biology and remains in widespread use today. The breakthrough earned him the 1980 Nobel Prize in Chemistry, which he shared with Walter Gilbert and Paul Berg. He is one of only three people to have won multiple Nobel Prizes in the same category (the others being John Bardeen in physics and Karl Barry Sharpless in chemistry), and one of five persons with two Nobel ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Daniel Nathans
Daniel Nathans (October 30, 1928 – November 16, 1999) was an American microbiologist. He shared the 1978 Nobel Prize in Physiology or Medicine for the discovery of restriction enzymes and their application in restriction mapping. Early life and education Nathans was born in Wilmington, Delaware, the last of nine children born to Russian Jewish immigrant parents, Sarah (Levitan) and Samuel Nathans. During the Great Depression his father lost his small business and was unemployed for a long time. Nathans attended public schools and then to the University of Delaware, where he received his B.S. degree in chemistry in 1950. He received his M.D. degree from Washington University in St. Louis in 1954 and did a one-year internship at Presbyterian Medical Center with Robert Loeb. Wanting a break before his medical residency, Nathans became a clinical associate at the National Cancer Institute at the National Institutes of Health in Bethesda, Maryland. There he split his time b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Noreen Murray
Noreen Elizabeth, Lady Murray (; 26 February 1935 – 12 May 2011) was an English molecular geneticist who helped pioneer recombinant DNA technology (genetic engineering) by creating a series of bacteriophage lambda vectors into which genes could be inserted and expressed in order to examine their function. During her career she was recognised internationally as a pioneer and one of Britain's most distinguished and highly respected molecular geneticists. Until her 2001 retirement she held a personal chair in molecular genetics at the University of Edinburgh.. She was president of the Genetical Society, vice president of the Royal Society, and a member of the UK Science and Technology Honours Committee. Education Noreen Parker was brought up in the village of Read, Lancashire, then from the age of five in Bolton-le-Sands. She was educated at Lancaster Girls' Grammar School, at King's College London ( BSc), and received her PhD from the University of Birmingham in 1959. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Kenneth Murray (biologist)
Sir Kenneth "Ken" Murray (30 December 1930 – 7 April 2013) was a British molecular biologist and the Biogen Professor of Molecular Biology at the University of Edinburgh. An important early figure in genetic engineering, Murray cofounded Biogen. There, he and his team developed one of the first vaccines against hepatitis B. Along with his wife, biologist Lady Noreen (née Parker), Murray also founded the Darwin Trust of Edinburgh, a charity supporting young biologists in their doctoral studies. The Telegraph, 14 April 2013 Education and career Murray achieved a 1st class honours degree in chemistry followed by PhD from the |
Hamilton O
Hamilton may refer to: * Alexander Hamilton (1755/1757–1804), first U.S. Secretary of the Treasury and one of the Founding Fathers of the United States * ''Hamilton'' (musical), a 2015 Broadway musical by Lin-Manuel Miranda ** ''Hamilton'' (album), album based on the musical ** '' The Hamilton Mixtape'', album of music from the musical performed by various artists ** ''Hamilton'' (2020 film), a live film recording of the musical, featuring the original cast Hamilton may also refer to: People * Hamilton (name), a common British surname and occasional given name, usually of Scottish origin, including a list of persons with the surname ** The Duke of Hamilton, the premier peer of Scotland ** Lord Hamilton (other), several Scottish, Irish and British peers, and some members of the judiciary, who may be referred to simply as ''Hamilton'' ** Clan Hamilton, an ancient Scottish kindred * Hamílton (footballer, born 1980), Togolese footballer * Lewis Hamilton (race driver, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Thomas J
Clarence Thomas (born June 23, 1948) is an American lawyer and jurist who has served since 1991 as an associate justice of the Supreme Court of the United States. President George H. W. Bush Clarence Thomas Supreme Court nomination, nominated him to succeed Thurgood Marshall. After Marshall, Thomas is the second African Americans, African American to serve on the U.S. Supreme Court and has been its List of United States Supreme Court justices by time in office, longest-serving member since Anthony Kennedy's retirement in 2018. He has also been the Court's oldest member since Stephen Breyer retired in 2022. Thomas was born in Pin Point, Georgia. After his father abandoned the family, he was raised by his grandfather in a poor Gullah community near Savannah, Georgia. Growing up as a devout Catholic, Thomas originally intended to be a priest in the Catholic Church but became dissatisfied with its efforts to combat racism and abandoned his aspiration to join the clergy. He gradua ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Analytical Biochemistry (journal)
''Analytical Biochemistry'' is a Peer review, peer-reviewed scientific journal established in 1960. It covers the field of biochemistry. According to the ''Journal Citation Reports'', the journal has a 2014 impact factor of 2.219. Abstracting and Indexing The journal is abstracted and indexed in Analytical Abstracts, Biological Abstracts, Chemical Abstracts, Current Contents/Life Sciences, EMBASE, EMBiology, MEDLINE, Science Citation Index, and Scopus. References External links * Academic journals established in 1960 Biochemistry journals Elsevier academic journals English-language journals Biweekly journals {{biochem-journal-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Proper Noun
A proper noun is a noun that identifies a single entity and is used to refer to that entity ('' Africa''; ''Jupiter''; '' Sarah''; ''Walmart'') as distinguished from a common noun, which is a noun that refers to a class of entities (''continent, planet, person, corporation'') and may be used when referring to instances of a specific class (a ''continent'', another ''planet'', these ''persons'', our ''corporation''). Some proper nouns occur in plural form (optionally or exclusively), and then they refer to ''groups'' of entities considered as unique (the ''Hendersons'', the '' Everglades'', the '' Azores'', the ''Pleiades''). Proper nouns can also occur in secondary applications, for example modifying nouns (the ''Mozart'' experience; his ''Azores'' adventure), or in the role of common nouns (he's no ''Pavarotti''; a few would-be ''Napoleons''). The detailed definition of the term is problematic and, to an extent, governed by convention. A distinction is normally made in current l ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
