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Rhodococcus Erythropolis
''Rhodococcus erythropolis'' is an aerobic Gram-positive bacterium species in the genus Rhodococcus. The name ''Rhodococcus erythropolis'' is derived from its morphogenetic cycle from branching to rod and to coccus morphology, which explains the series of morphological changes this bacterium undergoing during growth and development processes. These bacterium are found in red and orange colonies when observed this explains the species name ''erythropolis'' which means "red city" in Greek. Characteristics and adaptability This bacterium is aerobic and can be found in soil surfaces where oxygen is abundant and is described as red and orange colored when they are cultured on a medium. This bacterium is non-motile and have an unusual cell envelope composition characterized by a high mycolic acid content, which helps to elevate the cell surface ability of hydrophobicity. This characteristic of the cell surface helps the bacterium to survive between polar and non-polar media. Diver ...
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Gram-positive Bacteria
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is used by microbiologists to place bacteria into two main categories, gram-positive (+) and gram-negative (−). Gram-positive bacteria have a thick layer of peptidoglycan within the cell wall, and gram-negative bacteria have a thin layer of peptidoglycan. Gram-positive bacteria retain the crystal violet stain used in the test, resulting in a purple color when observed through an optical microscope. The thick layer of peptidoglycan in the bacterial cell wall retains the stain after it has been fixed in place by iodine. During the decolorization step, the decolorizer removes crystal violet from all other cells. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage ...
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Catabolism
Catabolism () is the set of metabolic pathways that breaks down molecules into smaller units that are either oxidized to release energy or used in other anabolic reactions. Catabolism breaks down large molecules (such as polysaccharides, lipids, nucleic acids, and proteins) into smaller units (such as monosaccharides, fatty acids, nucleotides, and amino acids, respectively). Catabolism is the breaking-down aspect of metabolism, whereas anabolism is the building-up aspect. Cells use the monomers released from breaking down polymers to either construct new polymer molecules or degrade the monomers further to simple waste products, releasing energy. Cellular wastes include lactic acid, acetic acid, carbon dioxide, ammonia, and urea. The formation of these wastes is usually an oxidation process involving a release of chemical free energy, some of which is lost as heat, but the rest of which is used to drive the synthesis of adenosine triphosphate (ATP). This molecule acts as a way ...
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Plasmid
A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria and archaea; however plasmids are sometimes present in and eukaryotic organisms as well. Plasmids often carry useful genes, such as those involved in antibiotic resistance, virulence, secondary metabolism and bioremediation. While chromosomes are large and contain all the essential genetic information for living under normal conditions, plasmids are usually very small and contain additional genes for special circumstances. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms. In the laboratory, plasmids may be introduced into a cell via transformation. Synthetic plasmids are available for procurement over the internet by various vendors ...
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Rhizosphere
The rhizosphere is the narrow region of soil or Substrate (biology), substrate that is directly influenced by root secretions and associated soil microorganisms known as the root microbiome. Pore space in soil, Soil pores in the rhizosphere can contain many bacteria and other microorganisms that feed on sloughed-off plant cells, termed ''rhizodeposition'', and the proteins and sugars released by roots, termed Root mucilage, root exudates. This symbiosis leads to more complex interactions, influencing plant growth and competition for resources. Much of the nutrient cycle, nutrient cycling and disease suppression by antibiotics required by plants occurs immediately adjacent to roots due to root exudates and metabolism, metabolic products of symbiotic and Plant pathology, pathogenic communities of microorganisms. The rhizosphere also provides space to produce Allelopathy, allelochemicals to control neighbours and relatives. The ''rhizoplane'' refers to the root surface including it ...
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Phyllosphere
In microbiology, the phyllosphere is the total above-ground surface of a plant when viewed as a habitat for microorganisms.Leveau, Johan H.J. (2006"Microbial communities in the phyllosphere" In: Riederer M. and Müller C. (Eds) ''Biology of the Plant Cuticle'', chapter 11, pages 334–367. The phyllosphere can be further subdivided into the caulosphere (stems), phylloplane (leaves), anthosphere (flowers), and carposphere (fruits). The below-ground microbial habitats (i.e. the thin-volume of soil surrounding root or subterranean stem surfaces) are referred to as the rhizosphere and laimosphere. Most plants host diverse communities of microorganisms including bacteria, fungi, archaea, and protists. Some are beneficial to the plant, while others function as plant pathogens and may damage the host plant or even kill it. The phyllosphere microbiome The leaf surface, or phyllosphere, harbours a microbiome comprising diverse communities of bacteria, archaea, fungi, algae and viruses ...
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Gram-negative Bacteria
Gram-negative bacteria are bacteria that, unlike gram-positive bacteria, do not retain the Crystal violet, crystal violet stain used in the Gram staining method of bacterial differentiation. Their defining characteristic is that their cell envelope consists of a thin peptidoglycan gram-negative cell wall, cell wall sandwiched between an inner (Cytoplasm, cytoplasmic) Cell membrane, membrane and an Bacterial outer membrane, outer membrane. These bacteria are found in all environments that support life on Earth. Within this category, notable species include the model organism ''Escherichia coli'', along with various pathogenic bacteria, such as ''Pseudomonas aeruginosa'', ''Chlamydia trachomatis'', and ''Yersinia pestis''. They pose significant challenges in the medical field due to their outer membrane, which acts as a protective barrier against numerous Antibiotic, antibiotics (including penicillin), Detergent, detergents that would normally damage the inner cell membrane, and the ...
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Bioremediation
Bioremediation broadly refers to any process wherein a biological system (typically bacteria, microalgae, fungi in mycoremediation, and plants in phytoremediation), living or dead, is employed for removing environmental pollutants from air, water, soil, fuel gasses, industrial effluents etc., in natural or artificial settings. The natural ability of organisms to adsorb, accumulate, and degrade common and emerging pollutants has attracted the use of biological resources in treatment of contaminated environment. In comparison to conventional physicochemical treatment methods bioremediation may offer advantages as it aims to be sustainable, eco-friendly, cheap, and scalable. This technology is rarely implemented however because it is slow or inefficient. Most bioremediation is inadvertent, involving native organisms. Research on bioremediation is heavily focused on stimulating the process by inoculation of a polluted site with organisms or supplying nutrients to promote their growt ...
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Oligotroph
An oligotroph is an organism that can live in an environment that offers very low levels of nutrients. They may be contrasted with copiotrophs, which prefer nutritionally rich environments. Oligotrophs are characterized by slow growth, low rates of metabolism, and generally low population density. Oligotrophic environments are those that offer little to sustain life. These environments include deep oceanic sediments, caves, glacial and polar ice, deep subsurface soil, aquifers, ocean waters, and leached soils. Examples of oligotrophic organisms are the cave-dwelling olm; the bacterium " ''Candidatus'' Pelagibacter communis", which is the most abundant organism in the ocean (with an estimated 2 × 1028 individuals in total); and lichens, with their extremely low metabolic rate. Etymology Etymologically, the word "oligotroph" is a combination of the Greek adjective ''oligos'' (ὀλίγος) meaning "few" and the adjective ''trophikos'' (τροφικός) meaning "feeding". Plant ...
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Rhodococcus
''Rhodococcus'' is a genus of aerobic, nonsporulating, nonmotile Gram-positive bacteria closely related to ''Mycobacterium'' and ''Corynebacterium''. While a few species are pathogenic, most are benign, and have been found to thrive in a broad range of environments, including soil, water, and eukaryotic cells. Some species have large genomes, including the 9.7 megabasepair genome (67% G/C) of ''Rhodococcus'' sp. RHA1. Strains of ''Rhodococcus'' are important owing to their ability to catabolize a wide range of compounds and produce bioactive steroids, acrylamide, and acrylic acid, and their involvement in fossil fuel biodesulfurization. This genetic and catabolic diversity is not only due to the large bacterial chromosome, but also to the presence of three large linear plasmids. ''Rhodococcus'' is also an experimentally advantageous system owing to a relatively fast growth rate and simple developmental cycle, but is not well characterized. Another important application of ''Rh ...
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Polychlorinated Biphenyl
Polychlorinated biphenyls (PCBs) are organochlorine compounds with the formula Carbon, C12Hydrogen, H10−''x''Chloride, Cl''x''; they were once widely used in the manufacture of carbonless copy paper, as heat transfer fluids, and as dielectric and coolant fluids for electrical equipment. They are highly toxic and carcinogenic chemical compounds, formerly used in industrial and consumer electronic products, whose production was banned internationally by the Stockholm Convention on Persistent Organic Pollutants in 2001. Because of their longevity, PCBs are still widely in use, even though their manufacture has declined drastically since the 1960s, when a multitude of problems was identified. With the discovery of PCBs' environmental toxicity, and classification as persistent organic pollutants, their production was banned for most uses by United States federal law on January 1, 1978. The International Agency for Research on Cancer (IARC) rendered PCBs as definite carcinogens i ...
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Polycyclic Aromatic Hydrocarbon
A Polycyclic aromatic hydrocarbon (PAH) is any member of a class of organic compounds that is composed of multiple fused aromatic rings. Most are produced by the incomplete combustion of organic matter— by engine exhaust fumes, tobacco, incinerators, in roasted meats and cereals, or when biomass burns at lower temperatures as in forest fires. The simplest representative is naphthalene, having two aromatic rings, and the three-ring compounds anthracene and phenanthrene. PAHs are uncharged, non-polar and planar. Many are colorless. Many of them are also found in fossil fuel deposits such as coal and in petroleum. Exposure to PAHs can lead to different types of cancer, to fetal development complications, and to cardiovascular issues. Polycyclic aromatic hydrocarbons are discussed as possible starting materials for abiotic syntheses of materials required by the earliest forms of life. Nomenclature and structure The terms polyaromatic hydrocarbon, or polynuclear aromatic hydro ...
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