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Pseudomonas Oryzihabitans
''Pseudomonas oryzihabitans'' is a nonfermenting yellow-pigmented, gram-negative, rod-shaped bacterium that can cause sepsis, peritonitis, endophthalmitis, and bacteremia.Freney et al., "Postoperative Infant Septicemia Caused by Pseudomonas luteola (CDC Group Ve-1) and Pseudomonas oryzihabitans (CDC Group Ve-2)," Journal of Clinical Microbiology 26 (1988): 1241-1243 It is an opportunistic pathogen of humans and warm-blooded animals that is commonly found in several environmental sources, from soil to rice paddies. They can be distinguished from other nonfermenters by their negative oxidase reaction and aerobic character. This organism can infect individuals that have major illnesses, including those undergoing surgery or with catheters in their body.Lejbkowicz et al., "Bacteraemia and Sinusitis due to Flavimonas oryzihabitans Infection," Scandinavian Journal of Infectious Diseases 35 (2003): 411-414 Based on the 16S RNA analysis, these bacteria have been placed in the ''Pseudomon ...
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Gram-negative
Gram-negative bacteria are bacteria that, unlike gram-positive bacteria, do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. Their defining characteristic is that their cell envelope consists of a thin peptidoglycan cell wall sandwiched between an inner ( cytoplasmic) membrane and an outer membrane. These bacteria are found in all environments that support life on Earth. Within this category, notable species include the model organism '' Escherichia coli'', along with various pathogenic bacteria, such as '' Pseudomonas aeruginosa'', '' Chlamydia trachomatis'', and '' Yersinia pestis''. They pose significant challenges in the medical field due to their outer membrane, which acts as a protective barrier against numerous antibiotics (including penicillin), detergents that would normally damage the inner cell membrane, and the antimicrobial enzyme lysozyme produced by animals as part of their innate immune system. Furthe ...
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Antibiotics
An antibiotic is a type of antimicrobial substance active against bacteria. It is the most important type of antibacterial agent for fighting pathogenic bacteria, bacterial infections, and antibiotic medications are widely used in the therapy, treatment and antibiotic prophylaxis, prevention of such infections. They may either bactericide, kill or bacteriostatic agent, inhibit the growth of bacteria. A limited number of antibiotics also possess antiprotozoal activity. Antibiotics are not effective against viruses such as the ones which cause the common cold or influenza. Drugs which inhibit growth of viruses are termed antiviral drugs or antivirals. Antibiotics are also not effective against fungi. Drugs which inhibit growth of fungi are called antifungal drugs. Sometimes, the term ''antibiotic''—literally "opposing life", from the Greek language, Greek roots ἀντι ''anti'', "against" and βίος ''bios'', "life"—is broadly used to refer to any substance used against ...
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Pseudomonadales
The Pseudomonadales are an order of Pseudomonadota. A few members are pathogens, such as species of ''Pseudomonas'', ''Moraxella'', and '' Acinetobacter'', which may cause disease in humans, animals and plants. ''Pseudomonas'' The bacterial genus ''Pseudomonas'' includes the opportunistic human pathogen '' P. aeruginosa'', plant pathogenic bacteria, plant beneficial bacteria, ubiquitous soil bacteria with bioremediation capabilities and other species that cause spoilage of milk and dairy products. ''Pseudomonas aeruginosa'' can cause chronic opportunistic infections that have become increasingly apparent in immunocompromised patients and the ageing population of industrialised societies. The genome sequences of several pseudomonads have become available in recent years and researchers are beginning to use the data to make new discoveries about this bacterium. ''Acinetobacter'' The genus '' Acinetobacter'' is a group of Gram-negative, nonmotile and nonfermentative bacteria belongi ...
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MacConkey Agar
MacConkey agar is a selective and differential culture medium for bacteria. It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation. Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color. The media inhibits growth of gram-positive organisms with crystal violet and bile salts, allowing for the selection and isolation of gram-negative bacteria. The media detects lactose fermentation by enteric bacteria with the pH indicator neutral red. Contents It contains bile salts (to inhibit most gram-positive bacteria), crystal violet dye (which also inhibits certain gram-positive bacteria), and neutral red dye (which turns pink if the microbes are fermenting lactose). Composition: * Peptone – 17 g * Proteose peptone – 3 g * Lactose – 10 g * Bile salts – 1.5 g * Sodium chloride – 5 g * Neutral red – 0.03 g * Crystal violet – ...
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Flagellum
A flagellum (; : flagella) (Latin for 'whip' or 'scourge') is a hair-like appendage that protrudes from certain plant and animal sperm cells, from fungal spores ( zoospores), and from a wide range of microorganisms to provide motility. Many protists with flagella are known as flagellates. A microorganism may have from one to many flagella. A gram-negative bacterium '' Helicobacter pylori'', for example, uses its flagella to propel itself through the stomach to reach the mucous lining where it may colonise the epithelium and potentially cause gastritis, and ulcers – a risk factor for stomach cancer. In some swarming bacteria, the flagellum can also function as a sensory organelle, being sensitive to wetness outside the cell. Across the three domains of Bacteria, Archaea, and Eukaryota, the flagellum has a different structure, protein composition, and mechanism of propulsion but shares the same function of providing motility. The Latin word means " whip" to describe its ...
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Pseudomonas Luteola
''Pseudomonas luteola'' is an opportunistic pathogen, found ubiquitously in damp environments. Originally designated in the genus ''Chryseomonas'', the species has since been reassigned to the genus ''Pseudomonas''. Morphology ''Pseudomonas luteola'' is a Gram-negative, motile aerobe. Its motility is created by multitrichous flagella. They grow as rods of 0.8 μm to 2.5 μm.http://www.tgw1916.net/Pseudomonas/luteola.html Colonies produce a yellow-orange pigment. Optimal temperature for growth is 30 °C. Importantly for classification, it grows best on heart infusion agar supplemented with 5% horse blood. It is also able to grow on TSA, Nutrient Agar, Mac Conkey or CASA Agar. Biosorption ''Pseudomonas luteola'' can absorb certain heavy metals such as Cr(VI) and Al(III). Both ions are found in industrial wastewaters. These metals are specifically targeted by P. luteola strain TEM05. Under relatively acidic conditions (pH: 4 and 5 for each ion respectively). Experiments indic ...
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Environmental Factor
An environmental factor, ecological factor or eco factor is any factor, abiotic or biotic, that influences living organisms. Abiotic factors include ambient temperature, amount of sunlight, air, soil, water and pH of the water soil in which an organism lives. Biotic factors would include the availability of food organisms and the presence of biological specificity, competitors, predators, and parasites. Overall An organism's genotype (e.g., in the zygote) translated into the adult phenotype through development during an organism's ontogeny, and subject to influences by many environmental effects. In this context, a phenotype (or phenotypic trait) can be viewed as any definable and measurable characteristic of an organism, such as its body mass or Human skin color, skin color. Apart from the true Monogenic (genetics), monogenic genetic disorders, environmental factors may determine the development of disease in those genetically predisposed to a particular condition. Pollut ...
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Biofilms
A biofilm is a syntrophic community of microorganisms in which cells stick to each other and often also to a surface. These adherent cells become embedded within a slimy extracellular matrix that is composed of extracellular polymeric substances (EPSs). The cells within the biofilm produce the EPS components, which are typically a polymeric combination of extracellular polysaccharides, proteins, lipids and DNA. Because they have a three-dimensional structure and represent a community lifestyle for microorganisms, they have been metaphorically described as "cities for microbes". Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be common in natural, industrial, and hospital settings. They may constitute a microbiome or be a portion of it. The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. Biofilms c ...
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Sinkholes
A sinkhole is a depression or hole in the ground caused by some form of collapse of the surface layer. The term is sometimes used to refer to doline, enclosed depressions that are also known as shakeholes, and to openings where surface water enters into underground passages known as ''ponor'', swallow hole or swallet. A ''cenote'' is a type of sinkhole that exposes groundwater underneath. ''Sink'', and ''stream sink'' are more general terms for sites that drain surface water, possibly by infiltration into sediment or crumbled rock. Most sinkholes are caused by Karst topography, karst processes – the chemical dissolution of carbonate rocks, collapse or suffosion processes. Sinkholes are usually circular and vary in size from tens to hundreds of Metre, meters both in diameter and depth, and vary in form from soil-lined bowls to bedrock-edged chasms. Sinkholes may form gradually or suddenly, and are found worldwide. Formation Natural processes Sinkholes may capture surf ...
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Ampicillin
Ampicillin is an antibiotic belonging to the aminopenicillin class of the penicillin family. The drug is used to prevent and treat several bacterial infections, such as respiratory tract infections, urinary tract infections, meningitis, salmonellosis, and endocarditis. It may also be used to prevent group B streptococcal infection in newborns. It is used by mouth, by injection into a muscle, or intravenously. Common side effects include rash, nausea, and diarrhea. It should not be used in people who are allergic to penicillin. Serious side effects may include ''Clostridioides difficile'' colitis or anaphylaxis. While usable in those with kidney problems, the dose may need to be decreased. Its use during pregnancy and breastfeeding appears to be generally safe. Ampicillin was discovered in 1958 and came into commercial use in 1961. It is on the World Health Organization's List of Essential Medicines. The World Health Organization classifies ampicillin as critical ...
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Penicillins
Penicillins (P, PCN or PEN) are a group of β-lactam antibiotics originally obtained from ''Penicillium'' moulds, principally '' P. chrysogenum'' and '' P. rubens''. Most penicillins in clinical use are synthesised by '' P. chrysogenum'' using deep tank fermentation and then purified. A number of natural penicillins have been discovered, but only two purified compounds are in clinical use: penicillin G ( intramuscular or intravenous use) and penicillin V (given by mouth). Penicillins were among the first medications to be effective against many bacterial infections caused by staphylococci and streptococci. They are still widely used today for various bacterial infections, though many types of bacteria have developed resistance following extensive use. Ten percent of the population claims penicillin allergies, but because the frequency of positive skin test results decreases by 10% with each year of avoidance, 90% of these patients can eventually tolerate penicillin. ...
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