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Phase Contrast Microscopy
__NOTOC__ Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors. Photographic equipment and the human eye are only sensitive to amplitude variations. Without special arrangements, phase changes are therefore invisible. Yet, phase changes often convey important information. Phase-contrast microscopy is particularly important in biology. It reveals many cellular structures that are invisible with a bright-field microscope, as exem ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Frits Zernike
Frits Zernike (; 16 July 1888 – 10 March 1966) was a Dutch physicist who received the Nobel Prize in Physics in 1953 for his invention of the phase-contrast microscope. Early life and education Frederick "Frits" Zernike was born on 16 July 1888 in Amsterdam, Netherlands to Carl Friedrich August Zernike and Antje Dieperink. Both parents were teachers of mathematics, and he especially shared his father's passion for physics. In 1905 he enrolled at the University of Amsterdam, studying chemistry (his major), mathematics and physics. Academic career In 1912, he was awarded a prize for his work on opalescence in gases. In 1913, he became assistant to Jacobus Kapteyn at the astronomical laboratory of Groningen University. In 1914, Zernike and Leonard Ornstein were jointly responsible for the derivation of the Ornstein–Zernike equation in critical-point theory. In 1915, he became lector in theoretical mechanics and mathematical physics at the same university and in 1920 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell Division
Cell division is the process by which a parent cell (biology), cell divides into two daughter cells. Cell division usually occurs as part of a larger cell cycle in which the cell grows and replicates its chromosome(s) before dividing. In eukaryotes, there are two distinct types of cell division: a vegetative division (mitosis), producing daughter cells genetically identical to the parent cell, and a cell division that produces Haploidisation, haploid gametes for sexual reproduction (meiosis), reducing the number of chromosomes from two of each type in the diploid parent cell to one of each type in the daughter cells. Mitosis is a part of the cell cycle, in which, replicated chromosomes are separated into two new Cell nucleus, nuclei. Cell division gives rise to genetically identical cells in which the total number of chromosomes is maintained. In general, mitosis (division of the nucleus) is preceded by the S stage of interphase (during which the DNA replication occurs) and is f ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phase Imaging
Phase-contrast imaging is a method of image, imaging that has a range of different applications. It measures differences in the refractive index of different materials to differentiate between structures under analysis. In conventional Light microscopy#Optical microscopy, light microscopy, phase contrast can be employed to distinguish between structures of similar transparency, and to examine crystals on the basis of their birefringence, double refraction. This has uses in biological, medical and geological science. In X-ray computed tomography, X-ray tomography, the same physical principles can be used to increase image contrast by highlighting small details of differing refractive index within structures that are otherwise uniform. In Transmission Electron Microscopy, transmission electron microscopy (TEM), phase contrast enables very high resolution (HR) imaging, making it possible to distinguish features a few Angstrom apart (at this point highest resolution is 40 pm). Atomic ph ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phase Shift Image Of Cells In 3D
Phase or phases may refer to: Science *State of matter, or phase, one of the distinct forms in which matter can exist *Phase (matter), a region of space throughout which all physical properties are essentially uniform *Phase space, a mathematical space in which each possible state of a physical system is represented by a point also referred to as a "microscopic state" **Phase space formulation, a formulation of quantum mechanics in phase space *Phase (waves), the position of a point in time (an instant) on a waveform cycle **Instantaneous phase, generalization for both cyclic and non-cyclic phenomena *AC phase, the phase offset between alternating current electric power in multiple conducting wires **Single-phase electric power, distribution of AC electric power in a system where the voltages of the supply vary in unison **Three-phase electric power, a common method of AC electric power generation, transmission, and distribution *Phase problem, the loss of information (the phase) ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
S Cerevisiae Under DIC Microscopy
S, or s, is the nineteenth letter of the Latin alphabet, used in the English alphabet, the alphabets of other western European languages and other latin alphabets worldwide. Its name in English is ''ess'' (pronounced ), plural ''esses''. History Northwest Semitic šîn represented a voiceless postalveolar fricative (as in 'ip'). It originated most likely as a pictogram of a tooth () and represented the phoneme via the acrophonic principle. Ancient Greek did not have a "sh" phoneme, so the derived Greek letter Sigma () came to represent the voiceless alveolar sibilant . While the letter shape Σ continues Phoenician ''šîn'', its name ''sigma'' is taken from the letter ''Samekh'', while the shape and position of ''samekh'' but name of ''šîn'' is continued in the '' xi''. Within Greek, the name of ''sigma'' was influenced by its association with the Greek word (earlier ), "to hiss". The original name of the letter "Sigma" may have been ''san'', but due to the e ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Neutral-density Filter
In photography and optics, a neutral-density filter, or ND filter, is a filter that reduces or modifies the intensity of all wavelengths, or colors, of light equally, giving no changes in hue of color rendition. It can be a colorless (clear) or grey filter, and is denoted by Wratten number 96. The purpose of a standard photographic neutral-density filter is to reduce the amount of light entering the lens. Doing so allows the photographer to select combinations of aperture, exposure time and sensor sensitivity that would otherwise produce overexposed pictures. This is done to achieve effects such as a shallower depth of field or motion blur of a subject in a wider range of situations and atmospheric conditions. For example, one might wish to photograph a waterfall at a slow shutter speed to create a deliberate motion-blur effect. The photographer might determine that to obtain the desired effect, a shutter speed of ten seconds was needed. On a very bright day, there might be s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Interference (wave Propagation)
In physics, interference is a phenomenon in which two coherent waves are combined by adding their intensities or displacements with due consideration for their phase difference. The resultant wave may have greater amplitude (constructive interference) or lower amplitude (destructive interference) if the two waves are in phase or out of phase, respectively. Interference effects can be observed with all types of waves, for example, light, radio, acoustic, surface water waves, gravity waves, or matter waves as well as in loudspeakers as electrical waves. Etymology The word ''interference'' is derived from the Latin words ''inter'' which means "between" and ''fere'' which means "hit or strike", and was used in the context of wave superposition by Thomas Young in 1801. Mechanisms The principle of superposition of waves states that when two or more propagating waves of the same type are incident on the same point, the resultant amplitude at that point is equal to the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Working Principle Of Phase Contrast Microscopy
Working may refer to: * Work (human activity), intentional activity people perform to support themselves, others, or the community Arts and media * ''Working'' (musical), a 1978 musical * ''Working'' (TV series), an American sitcom * ''Working'' (Caro book), a 2019 book by Robert Caro * ''Working'' (Terkel book), a 1974 book by Studs Terkel * ''Working!!'', a manga by Karino Takatsu * "Working" (song), by Tate McRae and Khalid, 2021 Engineering and technology * Cold working or cold forming, the shaping of metal below its recrystallization temperature * Hot working, the shaping of metal above its recrystallization temperature * Multiple working, having more than one locomotive under the control of one driver * Live-line working, the maintenance of electrical equipment while it is energised * Single-line working, using one train track out of two Other uses * Holbrook Working (1895–1985), statistician and economist * Working the system, exploiting rules and procedures for un ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Contrast (vision)
Contrast is the difference in luminance or color that makes an object (or its representation in an image or display) visible against a background of different luminance or color. The human visual system is more sensitive to contrast than to absolute luminance; thus, we can perceive the world similarly despite significant changes in illumination throughout the day or across different locations. The maximum contrast of an image is termed the contrast ratio or dynamic range. In images where the contrast ratio approaches the maximum possible for the medium, there is a ''conservation of contrast''. In such cases, increasing contrast in certain parts of the image will necessarily result in a decrease in contrast elsewhere. Brightening an image increases contrast in darker areas but decreases it in brighter areas; conversely, darkening the image will have the opposite effect. Bleach bypass reduces contrast in the darkest and brightest parts of an image while enhancing luminance contr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Light Scattering
In physics, scattering is a wide range of physical processes where moving particles or radiation of some form, such as light or sound, are forced to deviate from a straight trajectory by localized non-uniformities (including particles and radiation) in the medium through which they pass. In conventional use, this also includes deviation of reflected radiation from the angle predicted by the law of reflection. Reflections of radiation that undergo scattering are often called '' diffuse reflections'' and unscattered reflections are called '' specular'' (mirror-like) reflections. Originally, the term was confined to light scattering (going back at least as far as Isaac Newton in the 17th century). As more "ray"-like phenomena were discovered, the idea of scattering was extended to them, so that William Herschel could refer to the scattering of "heat rays" (not then recognized as electromagnetic in nature) in 1800. John Tyndall, a pioneer in light scattering research, noted the con ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Condenser (optics)
A condenser is an optical Lens (optics), lens that renders a beam divergence, divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes. The concept is applicable to all kinds of radiation undergoing optical transformation, such as electrons in electron microscopy, neutron radiation, and synchrotron radiation optics. Microscope condenser Condensers are located above the light source and under the sample in an upright microscope, and above the stage and below the light source in an inverted microscope. They act to gather light from the microscope's light source and concentrate it into a cone of light that illuminates the specimen. The aperture and angle of the light cone must be adjusted (via the size of the diaphragm) for each different objective lens with different numerical apertures. Condensers ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dark Field And Phase Contrast Microscopies
Darkness is the condition resulting from a lack of illumination, or an absence of visible light. Human vision is unable to distinguish colors in conditions of very low luminance because the hue-sensitive photoreceptor cells on the retina are inactive when light levels are insufficient, in the range of visual perception referred to as scotopic vision. The emotional response to darkness has generated metaphorical usages of the term in many cultures, often used to describe an unhappy or foreboding feeling. "Darkness" may also refer to night, which occurs when the Sun is more than 18° below the horizon. Scientific Perception The perception of darkness differs from the mere absence of light that sometimes lead to afterimages. In perceiving, the eye is active, and the part of the retina that is unstimulated produces a complementary afterimage. Physics In terms of physics, an object is said to be dark when it absorbs photons, causing it to appear dim compared to other objects. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
