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Oxalobacter Formigenes
''Oxalobacter formigenes'' is a Gram-negative bacteria, Gram negative oxalate-degrading Anaerobic organism, anaerobic bacterium that was first isolated from the gastrointestinal tract of a sheep in 1985. To date, the bacterium has been found to colonize the large intestines of numerous vertebrates, including humans, and has even been isolated from freshwater sediment. It processes oxalate by decarboxylation into formate (oxalyl-CoA decarboxylase), producing energy for itself in the process. The broad-spectrum antibiotic, broad-spectrum quinolone antibiotics kill ''O. formigenes''. If a person's Human gastrointestinal tract, gastrointestinal (GI) tract lacks this bacterium, and therefore lacks the primary source of the oxalyl-CoA decarboxylase enzyme, then the GI tract cannot degrade dietary oxalates; after some vitamin B6-modulated partial metabolic degradation in the body, the oxalates are excreted in the kidney, where they precipitates to form calcium oxalate kidney stones. '' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram-negative Bacteria
Gram-negative bacteria are bacteria that, unlike gram-positive bacteria, do not retain the Crystal violet, crystal violet stain used in the Gram staining method of bacterial differentiation. Their defining characteristic is that their cell envelope consists of a thin peptidoglycan gram-negative cell wall, cell wall sandwiched between an inner (Cytoplasm, cytoplasmic) Cell membrane, membrane and an Bacterial outer membrane, outer membrane. These bacteria are found in all environments that support life on Earth. Within this category, notable species include the model organism ''Escherichia coli'', along with various pathogenic bacteria, such as ''Pseudomonas aeruginosa'', ''Chlamydia trachomatis'', and ''Yersinia pestis''. They pose significant challenges in the medical field due to their outer membrane, which acts as a protective barrier against numerous Antibiotic, antibiotics (including penicillin), Detergent, detergents that would normally damage the inner cell membrane, and the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Formyl-CoA Transferase
In enzymology, a formyl-CoA transferase () is an enzyme that catalyzes the chemical reaction :formyl-CoA + oxalate \rightleftharpoons formate + oxalyl-CoA Thus, the two substrates of this enzyme are formyl-CoA and oxalate, whereas its two products are formate and oxalyl-CoA. This enzyme belongs to the family of transferases, specifically the CoA-transferases. The systematic name A systematic name is a name given in a systematic way to one unique group, organism, object or chemical substance, out of a specific population or collection. Systematic names are usually part of a nomenclature. A semisystematic name or semitrivi ... of this enzyme class is formyl-CoA:oxalate CoA-transferase. Other names in common use include formyl-coenzyme A transferase, and formyl-CoA oxalate CoA-transferase. Structural studies As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes , , , and . References * * EC 2.8.3 Enzymes of known str ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Secretagogue
In endocrinology, secretagogue is a substance that causes another substance to be secreted. The word contains the suffix '' -agogue'', which refers to something that ''leads'' to something else; a secretagogue thus leads to secretion. One example is gastrin, at Dictionary which stimulates the H/K ATPase in the parietal cells (increased productio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Primary Hyperoxaluria
Primary hyperoxaluria is a rare condition (autosomal recessive) resulting in increased excretion of oxalate (up to 600 mg a day from normal 50 mg a day), with oxalate stones being common. Signs and symptoms Primary hyperoxaluria is an autosomal recessive disease, meaning both copies of the gene contain the mutation. Both parents must have one copy of this mutated gene to pass it on to their child, but they do not typically show signs or symptoms of the disease. A single kidney stone in children or recurrent stones in adults is often the first warning sign of primary hyperoxaluria. Other symptoms range from recurrent urinary tract infections, severe abdominal pain or pain in the side, blood in the urine, to chronic kidney disease and kidney failure. The age of symptom onset, progression and severity can vary greatly from one person to another, even among members of the same family. Some individuals may have mild cases that go undiagnosed well into adulthood; others may de ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Halogeton Glomeratus
''Halogeton glomeratus'' is a species of flowering plant in the family Amaranthaceae known by the common names saltlover, Aral barilla, and halogeton. It is native to Russia, Central Asia and China, but the plant is probably better known in the western United States, where it is an introduced species and a notorious noxious weed. This annual herb is a hardy halophyte, thriving in soils far too saline to support many other plants. It also grows in alkali soils such as those on alkali flats and disturbed, barren habitat. It can be found in sagebrush and shadscale habitat, and it grows well in areas with cold winters. This plant produces a usually erect stem with several curving branches up to about tall. It has a taproot reaching up to half a meter deep in the soil and many lateral roots. The branches are lined with narrow, fleshy, blue-green leaves each up to about 2 centimeters long tipped with stiff bristles. The inflorescences are located all along the stem branches next to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Real-time Polymerase Chain Reaction
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively and semi-quantitatively (i.e., above/below a certain amount of DNA molecules). Two common methods for the detection of PCR products in real-time PCR are (1) non-specific fluorescent dyes that Intercalation (biochemistry), intercalate with any double-stranded DNA and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescence, fluorescent reporter, which permits detection only after nucleic acid hybridisation, hybridization of the probe with its complementary sequence. The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abb ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Brain Heart Infusion
Brain heart infusion (BHI) is a growth medium for growing microorganisms. It is a nutrient-rich medium, and can therefore be used to culture a variety of fastidious organisms. In particular, it has been used to culture streptococci, pneumococci and meningococci, which can be otherwise challenging to grow. BHI is made by combining an infusion from boiled bovine or porcine heart and brain with a variety of other nutrients. BHI broth is often used in food safety, water safety, and antibiotic sensitivity tests. Uses BHI is broadly used for culturing a variety of microorganisms, both in clinical and research settings. A number of fastidious organisms, including some bacteria, yeasts, and other fungi, grow well on BHI. It can also be used to differentiate between enterococci and group D streptococci. History The earliest version of brain heart infusion media was made in 1899 when Edward Rosenow combined dextrose broth with calf brain tissue to grow streptococci. This was modi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Yeast Extract
Yeast extracts consist of the cell contents of yeast without the cell walls; they are used as food additives or flavorings, or as nutrients for bacterial culture Growing media, media. They are often used to create savoury flavors and umami taste sensations and can be found in a large variety of packaged foods including frozen meals, crackers, snack foods, gravy, stock and more. They are rich in B vitamins (but not Vitamin B12, B12). Yeast extracts and Fermentation in food processing, fermented foods contain glutamic acid (free glutamates), an amino acid which adds an umami flavor. Glutamic acid is found in meat, cheese, fungi (mushrooms and yeast) and vegetables—such as broccoli and tomatoes. A number of other substances found in yeast extract provide aromas, some meat-like, when allowed to react under heat. The heat-autolytic process to make yeast extract of the autolysate type was invented in the 19th century by Justus von Liebig. Yeast cells are heated until they rupture, t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Acetate
An acetate is a salt formed by the combination of acetic acid with a base (e.g. alkaline, earthy, metallic, nonmetallic, or radical base). "Acetate" also describes the conjugate base or ion (specifically, the negatively charged ion called an anion) typically found in aqueous solution and written with the chemical formula . The neutral molecules formed by the combination of the acetate ion and a ''positive'' ion (called a cation) are also commonly called "acetates" (hence, ''acetate of lead'', ''acetate of aluminium'', etc.). The simplest of these is hydrogen acetate (called acetic acid) with corresponding salts, esters, and the polyatomic anion , or . Most of the approximately 5 million tonnes of acetic acid produced annually in industry are used in the production of acetates, which usually take the form of polymers. In nature, acetate is the most common building block for biosynthesis. Nomenclature and common formula When part of a salt, the formula of the acetate i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Carbonate
A carbonate is a salt of carbonic acid, (), characterized by the presence of the carbonate ion, a polyatomic ion with the formula . The word "carbonate" may also refer to a carbonate ester, an organic compound containing the carbonate group . The term is also used as a verb, to describe carbonation: the process of raising the concentrations of carbonate and bicarbonate ions in water to produce carbonated water and other carbonated beverageseither by the addition of carbon dioxide gas under pressure or by dissolving carbonate or bicarbonate salts into the water. In geology and mineralogy, the term "carbonate" can refer both to carbonate minerals and carbonate rock (which is made of chiefly carbonate minerals), and both are dominated by the carbonate ion, . Carbonate minerals are extremely varied and ubiquitous in chemically precipitated sedimentary rock. The most common are calcite or calcium carbonate, , the chief constituent of limestone (as well as the main component of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Glycerate Pathway
2-Phosphoglycolate (chemical formula C2H2O6P3-; also known as phosphoglycolate, 2-PG, or PG) is a natural metabolic product of the oxygenase reaction mediated by the enzyme ribulose 1,5-bisphosphate carboxylase (RuBisCo). Synthesis RuBisCo catalyzes the fixation of atmospheric carbon dioxide in the chloroplasts of plants. It uses ribulose 1,5-bisphosphate (RuBP) as substrate and facilitates carboxylation at the C2 carbon via an endiolate intermediate. The two three-carbon products ( 3-phosphoglycerate) are subsequently fed into the Calvin cycle. Atmospheric oxygen competes with this reaction. In a process called photorespiration RuBisCo can also catalyze addition of atmospheric oxygen to the C2 carbon of RuBP forming a high energy hydroperoxide intermediate that decomposes into 2-phosphoglycolate and 3-phosphoglycerate. Despite a higher energy barrier for the oxygenation reaction compared to carboxylation, photorespiration accounts for up to 25% of RuBisCo turnover in C3 pl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Oxalobacter Paraformigenes
''Oxalobacter paraformigenes'' is a Gram negative, non-spore-forming, oxalate-degrading anaerobic bacterium that was first isolated from human fecal samples. ''O. paraformigenes'' may have a role in calcium oxalate kidney stone disease because of its unique ability to utilize oxalate as its primary carbon source. Taxonomy ''Oxalobacter paraformigenes'' was originally thought to be a subgroup of ''Oxalobacter formigenes.'' Based on fatty acid profile, 16S ribosomal RNA sequencing, and DNA probes specific to the ''oxc'' ( oxalyl-CoA decarboxylase) gene and ''frc'' (formyl-CoA transferase In enzymology, a formyl-CoA transferase () is an enzyme that catalyzes the chemical reaction :formyl-CoA + oxalate \rightleftharpoons formate + oxalyl-CoA Thus, the two substrates of this enzyme are formyl-CoA and oxalate, whereas its two prod ...), ''O. paraformigenes'' strain HOxBLS was considered a group II strain. However, whole genome sequencing revealed that ''O. paraformigenes'' HOx ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
