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Kreft's Dichromaticity Index
Kreft's dichromaticity index (DI) is a measure for quantification of dichromatism. It is defined as the difference in hue angle (Δhab) between the color of the sample at the dilution, where the chroma (color saturation) is maximal, and the color of four times more diluted (or thinner) and four times more concentrated (or thicker) sample. The two hue angle differences are called the dichromaticity index towards lighter (Kreft's DIL) and dichromaticity index towards darker (Kreft's DID) respectively. Kreft's dichromaticity indexes DIL and DID for pumpkin seed oil, which is one of the most dichromatic substances, are −9 and −44, respectively. This means, that pumpkin seed oil changes its color from green-yellow to orange-red (for 44 degrees in Lab color space) when the thickness of the observed layer is increased from cca 0.5 mm to 2 mm; and it changes slightly towards green (for 9 degrees) if its thickness is reduced for four-fold. The color of pumpkin oil at incr ...
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Pumpkin Oil - CIELab
A pumpkin is a vernacular term for mature winter squash of species and varieties in the genus '' Cucurbita'' that has culinary and cultural significance but no agreed upon botanical or scientific meaning. The term ''pumpkin'' is sometimes used interchangeably with "squash" or "winter squash", and is commonly used for cultivars of '' Cucurbita argyrosperma'', '' Cucurbita ficifolia'', '' Cucurbita maxima'', '' Cucurbita moschata'', and '' Cucurbita pepo''. Native to North America (northeastern Mexico and the southern United States), ''C. pepo'' pumpkins are one of the oldest domesticated plants, having been used as early as 7,000 to 5,500 BC. Today, pumpkins of varied species are widely grown for food, as well as for aesthetic and recreational purposes. The pumpkin's thick shell contains edible seeds and pulp. Pumpkin pie, for instance, is a traditional part of Thanksgiving meals in Canada and the United States, and pumpkins are frequently carved as jack-o'-lanterns for de ...
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Rhodamine
Rhodamine is a family of related dyes, a subset of the triarylmethane dyes. They are derivatives of xanthene. Important members of the rhodamine family are Rhodamine 6G, Rhodamine 123, and Rhodamine B. They are mainly used to dye paper and inks, but they lack the lightfastness for fabric dyeing. Use Aside from their major applications, they are often used as a tracer dye, e.g. to determine the rate and direction of flow and transport of water. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine 123 is used in biochemistry to inhibit mitochondrion function. Rhodamine 123 appears to bind to the mitochondrial membranes and inhibit transport processes, especially the electron transport chain, thus slowing down cellular respiration. It is a ...
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Resazurin
Resazurin (7-Hydroxy-3''H''-phenoxazin-3-one 10-oxide) is a phenoxazine dye that is weakly fluorescent, nontoxic, cell-permeable, and redox‐sensitive. Resazurin has a blue to purple color (at pH > 6.5) and is used in microbiological, cellular, and enzymatic assays because it can be irreversibly reduced to the pink-colored and highly fluorescent resorufin (7-Hydroxy-3''H''-phenoxazin-3-one). At circum-neutral pH, resorufin can be detected by visual observation of its pink color or by fluorimetry, with an excitation maximum at 530-570 nm and an emission maximum at 580-590 nm. When a solution containing resorufin is submitted to reducing conditions (Eh < -110 mV), almost all resorufin is reversibly reduced to the translucid non-fluorescent dihydro ...
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Proflavin
Proflavine, also called proflavin and diaminoacridine, is an acriflavine derivative, a disinfectant bacteriostatic against many gram-positive bacteria. It has been used in the form of the dihydrochloride and hemisulfate salts as a topical antiseptic, and was formerly used as a urinary antiseptic. Proflavine is also known to have a mutagenic effect on DNA by intercalating between nucleic acid base pairs. It differs from most other mutagenic In genetics, a mutagen is a physical or chemical agent that permanently changes genetic material, usually DNA, in an organism and thus increases the frequency of mutations above the natural background level. As many mutations can cause cancer i ... components by causing basepair-deletions or basepair-insertions and not substitutions. In the presence of light, proflavine can induce double-stranded breaks in DNA. Proflavine absorbs strongly in the blue region at 445 nm (in water at pH 7) with molar extinction coefficient of ''c.'' 4 ...
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Nile Red
The Nile, , Bohairic , lg, Kiira , Nobiin: Áman Dawū is a major north-flowing river in northeastern Africa. It flows into the Mediterranean Sea. The Nile is the longest river in Africa and has historically been considered the longest river in the world, though this has been contested by research suggesting that the Amazon River is slightly longer.Amazon Longer Than Nile River, Scientists Say
Of the world's major rivers, the Nile is one of the smallest, as measured by annual flow in cubic metres of water. About long, its covers eleven countries: the
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Malachite Green
Malachite green is an organic compound that is used as a dyestuff and controversially as an antimicrobial in aquaculture. Malachite green is traditionally used as a dye for materials such as silk, leather, and paper. Despite its name the dye is not prepared from the mineral malachite; the name just comes from the similarity of color. Structures and properties Malachite green is classified in the dyestuff industry as a triarylmethane dye and also using in pigment industry. Formally, malachite green refers to the chloride salt 6H5C(C6H4N(CH3)2)2l, although the term malachite green is used loosely and often just refers to the colored cation. The oxalate salt is also marketed. The anions have no effect on the color. The intense green color of the cation results from a strong absorption band at 621 nm ( extinction coefficient of ). Malachite green is prepared by the condensation of benzaldehyde and dimethylaniline to give leuco malachite green (LMG): :C6H5CHO + C6H5N(CH ...
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Hemoglobin
Hemoglobin (haemoglobin BrE) (from the Greek word αἷμα, ''haîma'' 'blood' + Latin ''globus'' 'ball, sphere' + ''-in'') (), abbreviated Hb or Hgb, is the iron-containing oxygen-transport metalloprotein present in red blood cells (erythrocytes) of almost all vertebrates (the exception being the fish family Channichthyidae) as well as the tissues of some invertebrates. Hemoglobin in blood carries oxygen from the respiratory organs (''e.g.'' lungs or gills) to the rest of the body (''i.e.'' tissues). There it releases the oxygen to permit aerobic respiration to provide energy to power functions of an organism in the process called metabolism. A healthy individual human has 12to 20grams of hemoglobin in every 100mL of blood. In mammals, the chromoprotein makes up about 96% of the red blood cells' dry content (by weight), and around 35% of the total content (including water). Hemoglobin has an oxygen-binding capacity of 1.34mL O2 per gram, which increases the total blood oxygen ca ...
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Fluorescein
Fluorescein is an organic compound and dye based on the xanthene tricyclic structural motif, formally belonging to triarylmethine dyes family. It is available as a dark orange/red powder slightly soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications. The color of its aqueous solutions is green by reflection and orange by transmission (its spectral properties are dependent on pH of the solution), as can be noticed in bubble levels, for example, in which fluorescein is added as a colorant to the alcohol filling the tube in order to increase the visibility of the air bubble contained within (thus enhancing the precision of the instrument). More concentrated solutions of fluorescein can even appear red (because under these conditions nearly all incident emission is re-absorbed by the solution). It is on the World Health Organization's List of Essential Medicines. Uses Fluorescein sodium, the sodium salt of fluorescein, is used extens ...
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Crystal Violet
Crystal violet or gentian violet, also known as methyl violet 10B or hexamethyl pararosaniline chloride, is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Crystal violet has antibacterial, antifungal, and anthelmintic (vermicide) properties and was formerly important as a topical antiseptic. The medical use of the dye has been largely superseded by more modern drugs, although it is still listed by the World Health Organization. The name ''gentian violet'' was originally used for a mixture of methyl pararosaniline dyes (methyl violet), but is now often considered a synonym for ''crystal violet''. The name refers to its colour, being like that of the petals of certain gentian flowers; it is not made from gentians or violets. Production A number of possible routes can be used to prepare crystal violet. The original procedure developed by the German chemists Kern and Caro involved the reaction of dimethylaniline with phosgene ...
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