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Initiator Element
The initiator element (''Inr''), sometimes referred to as initiator motif, is a core promoter that is similar in function to the Pribnow box (in prokaryotes) or the TATA box (in eukaryotes). The ''Inr'' is the simplest functional promoter that is able to direct transcription initiation without a functional TATA box. It has the consensus sequence YYA+1NWYY in humans. Similarly to the TATA box, the Inr element facilitates the binding of transcription Factor II D ( TFIID). The Inr works by enhancing binding affinity and strengthening the promoter. Overview The initiator element (Inr) is the most common sequence found at the transcription start site (TSS) of eukaryotic genes. It was originally described as a 17 bp element in 1989, but other (newer ''and'' older) analyses have produced consensus sequences 2-9 bp in length. Inr in humans was first described in 1980 by Corden et al. as a broader TSS motif. It was first articulated and explained by two MIT biologists, Stephen T. Smal ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Core Promoter Elements
Core or cores may refer to: Science and technology * Core (anatomy), everything except the appendages * Core (laboratory), a highly specialized shared research resource * Core (manufacturing), used in casting and molding * Core (optical fiber), the signal-carrying portion of an optical fiber * Core, the central part of a fruit * Hydrophobic core, the interior zone of a protein * Nuclear reactor core, a portion containing the fuel components * Pit (nuclear weapon) or core, the fissile material in a nuclear weapon * Semiconductor intellectual property core (IP core), is a unit of design in ASIC/FPGA electronics and IC manufacturing * Atomic core, an atom with no valence electrons * Lithic core, in archaeology, a stone artifact left over from toolmaking Geology and astrophysics * Core sample, in Earth science, a sample obtained by coring ** Ice core * Core, the central part of a galaxy; see Mass deficit * Core (anticline), the central part of an anticline or syncline * Planetary core ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hydrogen Bond
In chemistry, a hydrogen bond (H-bond) is a specific type of molecular interaction that exhibits partial covalent character and cannot be described as a purely electrostatic force. It occurs when a hydrogen (H) atom, Covalent bond, covalently bonded to a more Electronegativity, electronegative donor atom or group (Dn), interacts with another electronegative atom bearing a lone pair of electrons—the hydrogen bond acceptor (Ac). Unlike simple Dipole–dipole attraction, dipole–dipole interactions, hydrogen bonding arises from charge transfer (nB → σ*AH), Atomic orbital, orbital interactions, and quantum mechanical Delocalized electron, delocalization, making it a resonance-assisted interaction rather than a mere electrostatic attraction. The general notation for hydrogen bonding is Dn−H···Ac, where the solid line represents a polar covalent bond, and the dotted or dashed line indicates the hydrogen bond. The most frequent donor and acceptor atoms are nitrogen (N), oxyg ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Adenosine
Adenosine (symbol A) is an organic compound that occurs widely in nature in the form of diverse derivatives. The molecule consists of an adenine attached to a ribose via a β-N9- glycosidic bond. Adenosine is one of the four nucleoside building blocks of RNA (and its derivative deoxyadenosine is a building block of DNA), which are essential for all life on Earth. Its derivatives include the energy carriers adenosine mono-, di-, and triphosphate, also known as AMP/ADP/ATP. Cyclic adenosine monophosphate (cAMP) is pervasive in signal transduction. Adenosine is used as an intravenous medication for some cardiac arrhythmias. Adenosyl (abbreviated Ado or 5'-dAdo) is the chemical group formed by removal of the 5′-hydroxy (OH) group. It is found in adenosylcobalamin (an active form of vitamin B12) and as a radical in the radical SAM enzymes. Medical uses Supraventricular tachycardia In individuals with supraventricular tachycardia (SVT), adenosine is a first line trea ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sulfolobus
''Sulfolobus'' is a genus of microorganism in the family Sulfolobaceae. It belongs to the kingdom Thermoproteati of the Archaea domain. ''Sulfolobus'' species grow in volcanic springs with optimal growth occurring at pH 2–3 and temperatures of 75–80 °C, making them acidophiles and thermophiles respectively. ''Sulfolobus'' cells are irregularly shaped and flagellar. Species of ''Sulfolobus'' are generally named after the location from which they were first isolated, e.g. ''Sulfolobus solfataricus'' (now recombined as ''Saccharolobus solfataricus)'' was first isolated in the Solfatara volcano. Other species can be found throughout the world in areas of volcanic or geothermal activity, such as geological formations called mud pots, which are also known as ''solfatare'' (plural of solfatara). ''Sulfolobus'' as a model to study the molecular mechanisms of DNA replication When the first Archaeal genome, '' Methanococcus jannaschii'', had been sequenced completely i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Drosophila Melanogaster
''Drosophila melanogaster'' is a species of fly (an insect of the Order (biology), order Diptera) in the family Drosophilidae. The species is often referred to as the fruit fly or lesser fruit fly, or less commonly the "vinegar fly", "pomace fly", or "banana fly". In the wild, ''D. melanogaster'' are attracted to rotting fruit and fermenting beverages, and are often found in orchards, kitchens and pubs. Starting with Charles W. Woodworth's 1901 proposal of the use of this species as a model organism, ''D. melanogaster'' continues to be widely used for biological research in genetics, physiology, microbial pathogenesis, and Life history theory, life history evolution. ''D. melanogaster'' was the first animal to be Fruit flies in space, launched into space in 1947. As of 2017, six Nobel Prizes have been awarded to drosophilists for their work using the insect. ''Drosophila melanogaster'' is typically used in research owing to its rapid life cycle, relatively simple genetics with on ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sp1 Transcription Factor
Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the ''SP1'' gene. Function The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. post-translational modifications such as phosphorylation, acetylation, ''O''-GlcNAcylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. In the SV40 virus, Sp1 binds to the GC boxes in the regulatory sequence of the genome. Structure SP1 belongs to the Sp/KLF family of transcription factors. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains two glutamine-rich activation domains at its N-terminus that ar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Transcription Preinitiation Complex
The preinitiation complex (abbreviated PIC) is a complex of approximately 100 proteins that is necessary for the transcription (genetics), transcription of protein-coding genes in eukaryotes and archaea. The preinitiation complex positions RNA polymerase II (Pol II) at gene transcription start sites, DNA melting, denatures the DNA, and positions the DNA in the RNA polymerase II active site for transcription. The minimal PIC includes RNA polymerase II and six general transcription factors: Transcription factor II A, TFIIA, Transcription factor II B, TFIIB, Transcription factor II D, TFIID, Transcription factor II E, TFIIE, Transcription factor II F, TFIIF, and Transcription factor II H, TFIIH. Additional regulatory complexes (such as the mediator (coactivator), mediator coactivator and chromatin structure remodeling (RSC) complex, chromatin remodeling complexes) may also be components of the PIC. Preinitiation complexes are also formed during RNA Polymerase I and RNA Polymerase II ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
RNA Polymerase II
RNA polymerase II (RNAP II and Pol II) is a Protein complex, multiprotein complex that Transcription (biology), transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. It is one of the three RNA polymerase, RNAP enzymes found in the nucleus of eukaryote, eukaryotic cells. A 550 kDa complex of 12 subunits, RNAP II is the most studied type of RNA polymerase. A wide range of transcription factors are required for it to bind to upstream gene promoter (biology), promoters and begin transcription. Discovery Early studies suggested a minimum of two RNAPs: one which synthesized rRNA in the nucleolus, and one which synthesized other RNA in the nucleoplasm, part of the nucleus but outside the nucleolus. In 1969, biochemists Robert G. Roeder and William J. Rutter, William Rutter discovered there are total three distinct nuclear RNA polymerases, an additional RNAP that was responsible for transcription of some kind of RNA in the nucleoplasm. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
David Baltimore
David Baltimore (born March 7, 1938) is an American biologist, university administrator, and 1975 Nobel Prize in Physiology or Medicine, Nobel laureate in Physiology or Medicine. He is a professor of biology at the California Institute of Technology (Caltech), where he served as president from 1997 to 2006. He founded the Whitehead Institute and directed it from 1982 to 1990. In 2008, he served as president of the American Association for the Advancement of Science. At age 37, Baltimore won the Nobel Prize with Renato Dulbecco and Howard Martin Temin, Howard M. Temin "for their discoveries concerning the interaction between Oncovirus, tumour viruses and the genetic material of the cell", specifically the discovery of the enzyme reverse transcriptase. He has contributed to immunology, virology, cancer research, biotechnology, and recombinant DNA research. He has also trained many doctoral students and postdoctoral fellows, several of whom have gone on to notable and distinguishe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Transcription Factor II D
Transcription factor II D (TFIID) is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins. Before the start of transcription, the transcription Factor II D (TFIID) complex binds to the core promoter DNA of the gene through specific recognition of promoter sequence motifs, including the TATA box, Initiator, Downstream Promoter, Motif Ten, or Downstream Regulatory elements. Functions * Coordinates the activities of more than 70 polypeptides required for initiation of transcription by RNA polymerase II * Binds to the core promoter to position the polymerase properly * Serves as the scaffold for assembly of the remainder of the transcription complex * Acts as a c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Adenine
Adenine (, ) (nucleoside#List of nucleosides and corresponding nucleobases, symbol A or Ade) is a purine nucleotide base that is found in DNA, RNA, and Adenosine triphosphate, ATP. Usually a white crystalline subtance. The shape of adenine is complementary and pairs to either thymine in DNA or uracil in RNA. In cells adenine, as an independent molecule, is rare. It is almost always covalent bond, covalently bound to become a part of a larger biomolecule. Adenine has a central role in cellular respiration. It is part of adenosine triphosphate which provides the energy that drives and supports most activities in living cell (biology), cells, such as Protein biosynthesis, protein synthesis, chemical synthesis, muscle contraction, and nerve impulse propagation. In respiration it also participates as part of the cofactor (biochemistry), cofactors nicotinamide adenine dinucleotide, flavin adenine dinucleotide, and Coenzyme A. It is also part of adenosine, adenosine monophosphate, cy ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
