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Ff Phages
Ff phages (for ''F'' specific ''f''ilamentous phages) is a group of almost identical filamentous bacteriophage, filamentous phage (genus ''Inovirus'') including phages F1 phage, f1, fd, M13 phage, M13 and ZJ/2, which infect Gram-negative bacteria, bacteria bearing the Fertility factor (bacteria), F fertility factor. The Virus#Structure, virion (virus particle) is a flexible filament measuring about 6 by 900 nm, comprising a cylindrical protein tube protecting a single-stranded circular DNA molecule at its core. The phage codes for only 11 gene products, and is one of the simplest viruses known. It has been widely used to study fundamental aspects of molecular biology. George Smith and Greg Winter used f1 and fd for their work on phage display for which they were awarded a share of the 2018 Nobel Prize in Chemistry. Early experiments on Ff phages used M13 to identify gene functions, and M13 was also developed as a cloning vehicle, so the name M13 is sometimes used as an informal syn ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Filamentous Bacteriophage Fd
The word filament, which is descended from Latin ''filum'' meaning " thread", is used in English for a variety of thread-like structures, including: Astronomy * Galaxy filament, the largest known cosmic structures in the universe * Solar filament, a solar prominence seen against the disc of the sun Biology * Myofilament, filaments of myofibrils constructed from proteins * Protein filament, a long chain of protein subunits, such as those found in hair or muscle * Part of a stamen, the male part of a flower * Hypha, a thread-like cell in fungi and Actinobacteria * Filamentation, an elongation of individual bacterial cells Textiles * Filament fiber, fiber that comes in a continuous long length * Filament yarn, as opposed to spun yarn Media * ''Filament'' (magazine), a female-oriented erotica magazine * 2002 movie by Jinsei Tsuji * Filament (band), a musical group from Japan * Filament Games, a Wisconsin-based educational video game developer * Filament Productions, a production de ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Inoviridae Virion
Filamentous bacteriophages are a family of viruses (''Inoviridae'') that infect bacteria, or bacteriophages. They are named for their filamentous shape, a worm-like chain (long, thin, and flexible, reminiscent of a length of cooked spaghetti), about 6 nm in diameter and about 1000-2000 nm long. This distinctive shape reflects their viral replication, method of replication: the coat of the virion comprises five types of viral protein, which are located in the inner membrane of the host bacterium during phage assembly, and these proteins are added to the nascent virion's DNA as it is extruded through the membrane. The simplicity of filamentous phages makes them an appealing model organism for research in molecular biology, and they have also shown promise as tools in nanotechnology and immunology. Characteristics Filamentous bacteriophages are among the simplest living organisms known, with far fewer genes than the classical Caudovirales, tailed bacteriophages studied b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Membrane Biology
Membrane biology is the study of the biological and physiochemical characteristics of membranes, with applications in the study of cellular physiology. Membrane bioelectrical impulses are described by the Hodgkin cycle. Biophysics Membrane biophysics is the study of biological membrane structure and function using physical, computational, mathematical, and biophysical methods. A combination of these methods can be used to create phase diagrams of different types of membranes, which yields information on thermodynamic behavior of a membrane and its components. As opposed to membrane biology, membrane biophysics focuses on quantitative information and modeling of various membrane phenomena, such as lipid raft The cell membrane, plasma membranes of cells contain combinations of glycosphingolipids, cholesterol and protein Receptor (biochemistry), receptors organized in glycolipoprotein lipid microdomains termed lipid rafts. Their existence in cellular me ... formation, rates ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Membrane Proteins
Membrane proteins are common proteins that are part of, or interact with, biological membranes. Membrane proteins fall into several broad categories depending on their location. Integral membrane proteins are a permanent part of a cell membrane and can either penetrate the membrane (Transmembrane protein, transmembrane) or associate with one or the other side of a membrane (Integral monotopic protein, integral monotopic). Peripheral membrane proteins are transiently associated with the cell membrane. Membrane proteins are common, and medically important—about a third of all human proteins are membrane proteins, and these are targets for more than half of all drugs. Nonetheless, compared to other classes of proteins, determining membrane protein structures remains a challenge in large part due to the difficulty in establishing experimental conditions that can preserve the correct (Native state, native) Protein structure, conformation of the protein in isolation from its native ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phage Proteins
A bacteriophage (), also known informally as a phage (), is a virus that infects and replicates within bacteria. The term is derived . Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have structures that are either simple or elaborate. Their genomes may encode as few as four genes (e.g. MS2) and as many as hundreds of genes. Phages replicate within the bacterium following the injection of their genome into its cytoplasm. Bacteriophages are among the most common and diverse entities in the biosphere. Bacteriophages are ubiquitous viruses, found wherever bacteria exist. It is estimated there are more than 1031 bacteriophages on the planet, more than every other organism on Earth, including bacteria, combined. Viruses are the most abundant biological entity in the water column of the world's oceans, and the second largest component of biomass after prokaryotes, where up to 9x108 virions per millilitre have been found in microbial mats at t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Inoviridae
Filamentous bacteriophages are a family of viruses (''Inoviridae'') that infect bacteria, or bacteriophages. They are named for their filamentous shape, a worm-like chain (long, thin, and flexible, reminiscent of a length of cooked spaghetti), about 6 nm in diameter and about 1000-2000 nm long. This distinctive shape reflects their method of replication: the coat of the virion comprises five types of viral protein, which are located in the inner membrane of the host bacterium during phage assembly, and these proteins are added to the nascent virion's DNA as it is extruded through the membrane. The simplicity of filamentous phages makes them an appealing model organism for research in molecular biology, and they have also shown promise as tools in nanotechnology and immunology. Characteristics Filamentous bacteriophages are among the simplest living organisms known, with far fewer genes than the classical tailed bacteriophages studied by the phage group in the mid- ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Filamentous Bacteriophage
Filamentous bacteriophages are a family of viruses (''Inoviridae'') that infect bacteria, or bacteriophages. They are named for their filamentous shape, a worm-like chain (long, thin, and flexible, reminiscent of a length of cooked spaghetti), about 6 nm in diameter and about 1000-2000 nm long. This distinctive shape reflects their method of replication: the coat of the virion comprises five types of viral protein, which are located in the inner membrane of the host bacterium during phage assembly, and these proteins are added to the nascent virion's DNA as it is extruded through the membrane. The simplicity of filamentous phages makes them an appealing model organism for research in molecular biology, and they have also shown promise as tools in nanotechnology and immunology. Characteristics Filamentous bacteriophages are among the simplest living organisms known, with far fewer genes than the classical tailed bacteriophages studied by the phage group in the mid-2 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Angela Belcher
Angela M. Belcher is a materials scientist, biological engineer, and the James Mason Crafts Professor of Biological Engineering and Materials Science at the Massachusetts Institute of Technology (MIT) in Cambridge, Massachusetts, United States. She is director of the Biomolecular Materials Group at MIT, a member of the Koch Institute for Integrative Cancer Research, and a 2004 MacArthur Fellow. In 2019, she was named head of the Department of Biological Engineering at MIT. She was elected a member of the National Academy of Sciences in 2022. Early life and education Belcher grew up in San Antonio, Texas. She attended the University of California, Santa Barbara, where she received her bachelor's degree from the College of Creative Studies in 1991 and her Ph.D. in chemistry in 1997. Career After studying abalone shells, she worked with several colleagues at MIT and engineered a virus, known as the M13 bacteriophage whose target is usually ''Escherichia coli''. M13 can be made to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Walker Motifs
The Walker A and Walker B motifs are protein sequence motifs, known to have highly conserved three-dimensional structures. These were first reported in ATP-binding proteins by Walker and co-workers in 1982. Of the two motifs, the A motif is the main "P-loop" responsible for binding phosphate, while the B motif is a much less conserved downstream region. The P-loop is best known for its presence in ATP- and GTP-binding proteins, and is also found in a variety of proteins with phosphorylated substrates. Major lineages include: * RecA and rotor ATP synthase / ATPases (α and β subunits). * Nucleic acid-dependent ATPases: helicases, Swi2, and PhoH () * AAA proteins * STAND NTPases including MJ, PH, AP, and NACHT ATPases * ABC- PilT ATPases * Nucleotide kinases () * G domain proteins: G-proteins (transducin), myosin. Walker A motif Walker A motif, also known as the Walker loop, or P-loop, or phosphate-binding loop, is a motif in proteins that is associated with phospha ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Membrane Proteins
Membrane proteins are common proteins that are part of, or interact with, biological membranes. Membrane proteins fall into several broad categories depending on their location. Integral membrane proteins are a permanent part of a cell membrane and can either penetrate the membrane (Transmembrane protein, transmembrane) or associate with one or the other side of a membrane (Integral monotopic protein, integral monotopic). Peripheral membrane proteins are transiently associated with the cell membrane. Membrane proteins are common, and medically important—about a third of all human proteins are membrane proteins, and these are targets for more than half of all drugs. Nonetheless, compared to other classes of proteins, determining membrane protein structures remains a challenge in large part due to the difficulty in establishing experimental conditions that can preserve the correct (Native state, native) Protein structure, conformation of the protein in isolation from its native ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rolling Circle
Rolling circle replication (RCR) is a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA or RNA, such as plasmids, the genomes of bacteriophages, and the circular RNA genome of viroids. Some eukaryotic viruses also replicate their DNA or RNA via the rolling circle mechanism. As a simplified version of natural rolling circle DNA replication, replication, an isothermal DNA amplification technique, rolling circle amplification was developed. The RCA mechanism is widely used in molecular biology and biomedical nanotechnology, especially in the field of Biosensor, biosensing (as a method of signal amplification). Circular DNA replication Rolling circle DNA replication is initiated by an initiator protein encoded by the plasmid or bacteriophage DNA, which nicks one strand of the double-stranded, circular DNA molecule at a site called the double-strand origin, or DSO. The initiator protein remains bound to the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
