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Elizabethkingia Meningoseptica
''Elizabethkingia meningoseptica'' is a Gram-negative, rod-shaped bacterium widely distributed in nature (e.g. fresh water, salt water, or soil). It may be normally present in fish and frogs; it may be isolated from chronic infectious states, as in the sputum of cystic fibrosis patients. In 1959, American bacteriologist Elizabeth O. King (who isolated '' Kingella kingae'' in 1960) was studying unclassified bacteria associated with pediatric meningitis at the Centers for Disease Control and Prevention in Atlanta, when she isolated an organism (CDC group IIa) that she named ''Flavobacterium meningosepticum'' (''Flavobacterium'' means "the yellow bacillus" in Latin; ''meningosepticum'' likewise means "associated with meningitis and sepsis"). In 1994, it was reclassified in the genus ''Chryseobacterium'' and renamed ''Chryseobacterium meningosepticum''(''chryseos'' = "golden" in Greek, so ''Chryseobacterium'' means a golden/yellow rod similar to ''Flavobacterium''). In 2005, a 16S r ...
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Vancomycin
Vancomycin is a glycopeptide antibiotic medication used to treat certain bacterial infections. It is administered intravenously ( injection into a vein) to treat complicated skin infections, bloodstream infections, endocarditis, bone and joint infections, and meningitis caused by methicillin-resistant ''Staphylococcus aureus''. Blood levels may be measured to determine the correct dose. Vancomycin is also taken orally (by mouth) to treat ''Clostridioides difficile'' infections. When taken orally, it is poorly absorbed. Common side effects include pain in the area of injection and allergic reactions. Occasionally, hearing loss, low blood pressure, or bone marrow suppression occur. Safety in pregnancy is not clear, but no evidence of harm has been found, and it is likely safe for use when breastfeeding. It is a type of glycopeptide antibiotic and works by blocking the construction of a cell wall. Vancomycin was approved for medical use in the United States in 1 ...
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International Journal Of Systematic And Evolutionary Microbiology
The ''International Journal of Systematic and Evolutionary Microbiology'' is a peer-reviewed scientific journal covering research in the field of microbial systematics that was established in 1951. Its scope covers the taxonomy, nomenclature, identification, characterisation, culture preservation, phylogeny, evolution, and biodiversity of all microorganisms, including prokaryotes, yeasts and yeast-like organisms, protozoa and algae. The journal is currently published monthly by the Microbiology Society. An official publication of the International Committee on Systematics of Prokaryotes (ICSP) and International Union of Microbiological Societies (Bacteriology and Applied Microbiology Division), the journal is the single official international forum for the publication of new species names for prokaryotes.
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Indole Test
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to convert tryptophan into indole. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as "tryptophanase." Biochemistry Indole is generated by reductive deamination from tryptophan via the intermediate molecule indolepyruvic acid. Tryptophanase catalyzes the deamination reaction, during which the amine (-NH2) group of the tryptophan molecule is removed. Final products of the reaction are indole, pyruvic acid, ammonium (NH4+) and energy. Pyridoxal phosphate is required as a coenzyme. Performing a test Like many biochemical tests on bacteria, results of an indole test are indicated by a change in color following a reaction with an added reagent. Pure bacterial culture must be grown in sterile tryptophan or peptone broth for 24–48 hours before performing the test. Following incubation, five drops of K ...
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Oxidase Test
The oxidase test is used to determine whether an organism possesses the cytochrome c oxidase enzyme. The test is used as an aid for the differentiation of ''Neisseria'', ''Moraxella'', ''Campylobacter'' and ''Pasteurella'' species (oxidase positive). It is also used to differentiate pseudomonads from related species.MacFaddin JF, editor. Biochemical Tests for Identification of Medical Bacteria. 3rd ed. Philadelphia:Lippincott Williams and Wilkins; 2000. p. 363-7 Classification Strains may be either oxidase-positive (OX+) or oxidase-negative (OX-). OX+ OX+ normally means the bacterium contains cytochrome c oxidase (also known as Complex IV) and can therefore use oxygen for energy production by converting O2 to H2O2 or H2O with an electron transfer chain. The Pseudomonadaceae are typically OX+. The Gram-negative diplococci Neisseria and Moraxella are oxidase-positive. Many Gram-negative, spiral curved rods are also oxidase-positive, which includes ''Helicobacter pylori'', ''Vibrio ...
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Catalase Test
Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals) which catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second. Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. It contains four iron-containing heme groups that allow the enzyme to react with hydrogen peroxide. The optimum pH for human catalase is approximately 7, and has a fairly broad maximum: the rate of reaction does not change appreciably between pH 6.8 and 7.5. The pH optimum for other catalases varies between 4 and 11 depending on the species. The optimum temperature also varies by species. Structure Human catalase forms a t ...
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Obligate Aerobe
An obligate aerobe is an organism that requires oxygen to grow. Through cellular respiration, these organisms use oxygen to metabolise substances, like sugars or fats, to obtain energy. In this type of respiration, oxygen serves as the terminal electron acceptor for the electron transport chain. Aerobic respiration has the advantage of yielding more energy ( adenosine triphosphate or ATP) than fermentation or anaerobic respiration, but obligate aerobes are subject to high levels of oxidative stress."Obligate aerobe - definition from Biology-Online.org." ''Biology Online.'' Biology-Online, n.d. Web. 12 Dec 2009. Examples Among organisms, almost all animals, most fungi, and several bacteria are obligate aerobes. Examples of obligately aerobic bacteria include ''Mycobacterium tuberculosis'' (acid-fast), ''Bacillus'' (Gram-positive), and ''Nocardia asteroides'' (Gram-positive). With the exception of the yeasts, most fungi are obligate aerobes. Also, almost all algae are obliga ...
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Spore
In biology, a spore is a unit of sexual reproduction, sexual (in fungi) or asexual reproduction that may be adapted for biological dispersal, dispersal and for survival, often for extended periods of time, in unfavourable conditions. Spores form part of the Biological life cycle, life cycles of many plants, algae, fungus, fungi and protozoa. They were thought to have appeared as early as the mid-late Ordovician period as an adaptation of early land plants. Bacterial spores are not part of a sexual cycle, but are resistant structures used for survival under unfavourable conditions. Myxozoan spores release amoeboid infectious germs ("amoebulae") into their hosts for parasitic infection, but also reproduce within the hosts through the pairing of two nuclei within the plasmodium, which develops from the amoebula. In plants, spores are usually haploid and unicellular and are produced by meiosis in the sporangium of a diploid sporophyte. In some rare cases, a diploid spore is also p ...
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Gram Stain
Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. Gram staining differentiates bacteria by the chemical and physical properties of their Cell wall#Bacterial cell walls, cell walls. Gram-positive cells have a thick layer of peptidoglycan in the cell wall that retains the primary stain, crystal violet. Gram-negative cells have a thinner peptidoglycan layer that allows the crystal violet to wash out on addition of ethanol. They are stained pink or red by the counterstain, commonly safranin or fuchsine. Lugol's iodine solution is always added after addition of crystal violet to form a stable complex with crystal violet that strengthens the bonds of the stain with the cell wall. Gram staining is almost al ...
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Motility
Motility is the ability of an organism to move independently using metabolism, metabolic energy. This biological concept encompasses movement at various levels, from whole organisms to cells and subcellular components. Motility is observed in animals, microorganisms, and even some plant structures, playing crucial roles in activities such as foraging, reproduction, and cellular functions. It is genetically determined but can be influenced by environmental factors. In multicellular organisms, motility is facilitated by systems like the Nervous system, nervous and Human musculoskeletal system, musculoskeletal systems, while at the cellular level, it involves mechanisms such as amoeboid movement and flagellar propulsion. These cellular movements can be directed by external stimuli, a phenomenon known as taxis. Examples include chemotaxis (movement along chemical gradients) and phototaxis (movement in response to light). Motility also includes physiological processes like gastroi ...
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Bacillus (shape)
Bacterial cellular morphologies are the shapes that are characteristic of various types of bacteria and often key to their identification. Their direct examination under a light microscope enables the classification of these bacteria (and archaea). Generally, the basic morphologies are spheres (coccus) and round-ended cylinders or rod shaped (bacillus). But, there are also other morphologies such as helically twisted cylinders (example '' Spirochetes''), cylinders curved in one plane (selenomonads) and unusual morphologies (the square, flat box-shaped cells of the Archaean genus '' Haloquadratum)''. Other arrangements include pairs, tetrads, clusters, chains and palisades. Types Coccus A coccus (plural ''cocci'', from the Latin ''coccinus'' (scarlet) and derived from the Greek ''kokkos'' (berry)), is any microorganism (usually bacteria) whose overall shape is spherical or nearly spherical. Coccus refers to the shape of the bacteria and can contain multiple genera, such as st ...
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Gnetum Gnemon
''Gnetum'' is a genus of gymnosperms, the sole genus in the family Gnetaceae within the Gnetophyta. They are tropical evergreen trees, shrubs and lianas. Unlike other gymnosperms, they possess vessel elements in the xylem. Some species have been proposed to have been the first plants to be insect- pollinated as their fossils occur in association with extinct pollinating scorpionflies. Molecular phylogenies based on nuclear and plastid sequences from most of the species indicate hybridization among some of the Southeast Asian species. Fossil-calibrated molecular-clocks suggest that the ''Gnetum'' lineages now found in Africa, South America and Southeast Asia are the result of ancient long-distance dispersal across seawater. Their leaves are rich in phytochemicals such as flavonoids and stilbenes. Of the species studied so far, ''Gnetum'' have photosynthetic and transpiration capacities which are considerably lower than those of other seed plants, due to the absence of multipl ...
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Elizabethkingia
''Elizabethkingia'' is a genus of bacterium in the order of Flavobacteriales. It was established in 2005 from a branch in of the genus '' Chryseobacterium'', and named after Elizabeth O. King, the discoverer of the type species. ''Elizabethkingia'' has been found in soil, rivers, and reservoirs worldwide. The genus contains several pathogenic species, such as '' E. meningoseptica'' and '' E. anophelis''. Classification The genus includes four species: * '' Elizabethkingia anophelis'', isolated from ''Anopheles'' mosquitoes, can cause respiratory tract illness in humans,Wisconsin Department of Health Services:Wisconsin 2016 ''Elizabethkingia anophelis'' outbreak, March 11, 2016 the cause of a 2016 outbreak centered in Wisconsin. * '' Elizabethkingia endophytica'', isolated from blemished stems of sweet corn, ''Zea mays'' * ''Elizabethkingia meningoseptica'', can cause outbreaks of neonatal meningitis in premature newborns and infants * '' Elizabethkingia miricola'', isolated ...
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