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Optical Devices
An optical instrument is a device that processes light waves (or photons), either to enhance an image for viewing or to analyze and determine their characteristic properties. Common examples include periscopes, microscopes, telescopes, and cameras. Image enhancement The first optical instruments were telescopes used for magnification of distant images, and microscopes used for magnifying very tiny images. Since the days of Galileo and Van Leeuwenhoek, these instruments have been greatly improved and extended into other portions of the electromagnetic spectrum. The binocular device is a generally compact instrument for both eyes designed for mobile use. A camera could be considered a type of optical instrument, with the pinhole camera and camera obscura being very simple examples of such devices. Analysis Another class of optical instrument is used to analyze the properties of light or optical materials. They include: *Interferometer for measuring the interference properties of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Optical Devices England 1858
Optics is the branch of physics that studies the behaviour and properties of light, including its interactions with matter and the construction of optical instruments, instruments that use or Photodetector, detect it. Optics usually describes the behaviour of visible light, visible, ultraviolet, and infrared light. Light is a type of electromagnetic radiation, and other forms of electromagnetic radiation such as X-rays, microwaves, and radio waves exhibit similar properties. Most optical phenomena can be accounted for by using the Classical electromagnetism, classical electromagnetic description of light, however complete electromagnetic descriptions of light are often difficult to apply in practice. Practical optics is usually done using simplified models. The most common of these, geometric optics, treats light as a collection of Ray (optics), rays that travel in straight lines and bend when they pass through or reflect from surfaces. Physical optics is a more comprehensive mo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Polarimeter
A polarimeter is a scientific instrument used to measure optical rotation: the angle of rotation caused by passing linearly polarized light through an Optical activity, optically active substance. Some chemical substances are optically active, and linearly polarized (uni-directional) light will rotate either to the left (counter-clockwise) or right (clockwise) when passed through these substances. The amount by which the light is rotated is known as the ''angle of rotation''. The direction (clockwise or counterclockwise) and magnitude of the rotation reveals information about the sample's Chirality (chemistry), chiral properties such as the relative concentration of enantiomers present in the sample. History Polarization by reflection was discovered in 1808 by Étienne-Louis Malus (1775–1812). Measuring principle The ratio, the purity, and the concentration of two enantiomers can be measured via polarimetry. Enantiomers are characterized by their property to rotate the plan ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Surface Plasmon Resonance
Surface plasmon resonance (SPR) is a phenomenon that occurs where electrons in a thin metal sheet become excited by light that is directed to the sheet with a particular angle of incidence (optics), angle of incidence, and then travel parallel to the sheet. Assuming a constant light source wavelength and that the metal sheet is thin, the angle of incidence that triggers SPR is related to the refractive index of the material and even a small change in the refractive index will cause SPR to not be observed. This makes SPR a possible technique for detecting particular substances (analytes) and SPR biosensors have been developed to detect various important biomarkers. Explanation The surface plasmon polariton is a non-radiative Surface wave#Electromagnetic waves, electromagnetic surface wave that propagates in a direction parallel to the negative permittivity/dielectric material interface. Since the wave is on the boundary of the conductor and the external medium (air, water or vacuu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Fluorochrome
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to macromolecules, serving as a markers (or dyes, or tags, or reporters) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, such as fluorescent imaging and spectroscopy. Fluorescein, via its amine-reactive isothiocyanate derivative fluorescein isothiocyanate (FITC), has been one of the most popular fluorophores. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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DNA Sequencer
A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine). This is then reported as a text string, called a read. Some DNA sequencers can be also considered optical instruments as they analyze light signals originating from fluorochromes attached to nucleotides. The first automated DNA sequencer, invented by Lloyd M. Smith, was introduced by Applied Biosystems in 1987. It used the Sanger sequencing method, a technology which formed the basis of the "first generation" of DNA sequencers and enabled the completion of the human genome project in 2001. This first generation of DNA sequencers are essentially automated electrophoresis systems that detect the migration of labelled DNA fragments. Therefore, these sequencers can also be used in the genotyping of genetic markers where only the length of a DNA fragment( ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Vertometer
A lensmeter or lensometer (sometimes even known as focimeter or vertometer), is an optical instrument used in ophthalmology. It is mainly used by optometrists and opticians to measure the back or front vertex power of a spectacle lens and verify the correct prescription in a pair of eyeglasses, to properly orient and mark uncut lenses, and to confirm the correct mounting of lenses in spectacle frames. Lensmeters can also verify the power of contact lenses, if a special lens support is used. The parameters appraised by a lensmeter are the values specified by an ophthalmologist or optometrist on the patient's prescription: sphere, cylinder, axis, add, and in some cases, prism. The lensmeter is also used to check the accuracy of progressive lenses, and is often capable of marking the lens center and various other measurements critical to proper performance of the lens. It may also be used prior to an eye examination to obtain the last prescription the patient was given, in order ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Autocollimator
An autocollimator is an optical instrument for non-contact measurement of angles. They are typically used to align components and measure deflections in optical or mechanical systems. An autocollimator works by projecting an image onto a target mirror and measuring the deflection of the returned image against a scale, either visually or by means of an electronic detector. A visual autocollimator can measure angles as small as 1 arcsecond (4.85 microradians), while an electronic autocollimator can have up to 100 times more resolution. Visual autocollimators are often used for aligning laser rod ends and checking the face parallelism of optical windows and wedges. Electronic and digital autocollimators are used as angle measurement standards, for monitoring angular movement over long periods of time and for checking angular position repeatability in mechanical systems. Servo autocollimators are specialized compact forms of electronic autocollimators that are used in high-speed serv ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Visible Spectrum
The visible spectrum is the spectral band, band of the electromagnetic spectrum that is visual perception, visible to the human eye. Electromagnetic radiation in this range of wavelengths is called ''visible light'' (or simply light). The optical spectrum is sometimes considered to be the same as the visible spectrum, but some authors define the term more broadly, to include the ultraviolet and infrared parts of the electromagnetic spectrum as well, known collectively as ''optical radiation''. A typical human eye will respond to wavelengths from about 380 to about 750 nanometers. In terms of frequency, this corresponds to a band in the vicinity of 400–790 Terahertz (unit), terahertz. These boundaries are not sharply defined and may vary per individual. Under optimal conditions, these limits of human perception can extend to 310 nm (ultraviolet) and 1100 nm (near infrared). The spectrum does not contain all the colors that the human visual system can distinguish. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Monochromator
A monochromator is an optics, optical device that transmits a mechanically selectable narrow band of wavelengths of light or other radiation chosen from a wider range of wavelengths available at the input. The name is . Uses A device that can produce monochromatic light has many uses in science and in optics because many optical characteristics of a material are dependent on wavelength. Although there are a number of useful ways to select a narrow band of wavelengths (which, in the visible range, is perceived as a pure color), there are not as many other ways to easily select any wavelength band from a wide range. See #Applications, below for a discussion of some of the uses of monochromators. In hard X-ray and neutron radiation, neutron optics, crystal monochromators are used to define wave conditions on the instruments. Techniques A monochromator can use either the phenomenon of optical dispersion in a Prism (optics), prism, or that of diffraction using a diffraction gratin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Spectrometer
A spectrometer () is a scientific instrument used to separate and measure Spectrum, spectral components of a physical phenomenon. Spectrometer is a broad term often used to describe instruments that measure a continuous variable of a phenomenon where the spectral components are somehow mixed. In visible light a spectrometer can separate white light and measure individual narrow bands of color, called a spectrum. A mass spectrometer measures the spectrum of the masses of the atoms or molecules present in a gas. The first spectrometers were used to split light into an array of separate colors. Spectrometers were History_of_spectroscopy, developed in early studies of physics, astronomy, and chemistry. The capability of spectroscopy to determine Analytical_chemistry#Spectroscopy, chemical composition drove its advancement and continues to be one of its primary uses. Spectrometers are used in Astronomical spectroscopy, astronomy to analyze the chemical composition of Astronomical_spe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Refractive Index
In optics, the refractive index (or refraction index) of an optical medium is the ratio of the apparent speed of light in the air or vacuum to the speed in the medium. The refractive index determines how much the path of light is bent, or refraction, refracted, when entering a material. This is described by Snell's law of refraction, , where and are the angle of incidence (optics), angle of incidence and angle of refraction, respectively, of a ray crossing the interface between two media with refractive indices and . The refractive indices also determine the amount of light that is reflectivity, reflected when reaching the interface, as well as the critical angle for total internal reflection, their intensity (Fresnel equations) and Brewster's angle. The refractive index, n, can be seen as the factor by which the speed and the wavelength of the radiation are reduced with respect to their vacuum values: the speed of light in a medium is , and similarly the wavelength in that me ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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Refractometer
A refractometer is a laboratory or field device for the measurement of an Refractive index, index of refraction (refractometry). The index of refraction is calculated from the observed refraction angle using Snell's law. For mixtures, the index of refraction then allows the concentration to be determined using mixing rules such as the Gladstone–Dale relation and Clausius–Mossotti_relation#Lorentz–Lorenz_equation, Lorentz–Lorenz equation. Refractometry Standard refractometers measure the extent of light refraction (as part of a refractive index) of transparent substances in either a liquid this is then used in order to identify a liquid sample, analyze the sample's purity, and determine the amount or concentration of dissolved substances within the sample. As light passes through the liquid from the air it will slow down and create a ‘bending’ illusion, the severity of the ‘bend’ will depend on the amount of substance dissolved in the liquid. For example, the am ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |